In vivo simulated in vitro model of Jasminum sambac (Linn.) using mammalian liver slice technique

Kalaiselvi, M.; Narmadha, R.; Ragavendran, Paramasivam; Raj, A.Jude Jebri; Sophia, D; Rai, Govind Kumar; Gomathi, D.; Uma, C.; Kalaivani, K

doi:10.1016/s2221-1691(11)60158-5

Cited by 15 publications

(11 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early study [ 19 ] reported significant LPO inhibitory effect of hydroalcoholic leaf extract of J. sambac which further strengthens its claim as a cardioprotective agent as LPO is one of the major contributory factors in ADR induced MI [ 20 ].…”

Section: Discussionmentioning

confidence: 93%

Jasminum sambac: A Potential Candidate for Drug Development to Cure Cardiovascular Ailments

et al. 2021

View full text Add to dashboard Cite

Jasminum sambac (L.) is a South Asian folkloric medicinal plant that has traditionally been used to treat cardiovascular problems. The current investigation was meticulously organized to explore the pharmacological foundation for the medicinal uses of J. sambac pertaining to cardiovascular ailments and to investigate the core mechanisms. Mechanistic investigation revealed that crude leaf extract of J. sambac produced ex-vivo vasorelaxant effects in endotheliumintact aorta ring preparation and hypotensive effect was recorded via pressure and force transducers coupled to the Power Lab Data Acquisition System. Moreover; J. sambac showed cardioprotective effects against adrenaline -induced left ventricular hypertrophy in rabbits observed hemodynamic. CK-MB, LDH, troponin, CRP, ALT, AST, ALP levels were shown to be lower in the myocardial infarction model, as were necrosis, oedema, and inflammatory cell recruitment in comparison to control. J. sambac has shown good antioxidant potential as well as prolonged the noradrenaline induced platelet adhesion. The vasorelaxant and cardioprotective effects in both in vivo and ex vivo experiments, which are enabled by activation of muscarinic receptor and/or releasing the nitric oxide and by reducing the adrenaline, induced oxidative stress, justifying its usage in cardiovascular disorders.

show abstract

Section: Discussionmentioning

confidence: 93%

Jasminum sambac: A Potential Candidate for Drug Development to Cure Cardiovascular Ailments

et al. 2021

View full text Add to dashboard Cite

show abstract

“…HCC is the second leading cause of cancerrelated deaths in many Asian regions, with peak incidences in East Asia [7][8]. Despite recent scientific advancement in hepatology, liver problems have continued to increase [9]. According to 2014 epidemiological statistics from International Agency for Research on Cancer, 97.8 males and 61 females per 100,000 in Mongolia suffered from liver cancer; liver cancer occupied 78.8% of all the morbidity; and the mortality rate was registered as 7.4 times higher than the global average [10].…”

Section: Effects Of Hepaclin-4 On Hepatocellular Carcinoma (Hep G2) Imentioning

confidence: 99%

Effects of Hepaclin-4 on Hepatocellular Carcinoma (Hep G2)

Dalkh,

Naranbat,

Bayarsaikhan

et al. 2018

Cent. Asian j. Med. Sci.

View full text Add to dashboard Cite

Objective: Liver cancer is the fifth most frequent cancer worldwide and is usually diagnosed at an advanced stage. Natural products play an important role in anticancer drug therapy because they are effective in treating specific characteristics of cancer while also effective at reducing side effects. However, the cytotoxicity and anti-proliferative effects of Scutellaria baicalensis Georgi, Saussurea amara L, Chiazospermum erectum (L) Bernh, Carthamus tinctorius L., remain unclear. Our research aims to investigate the anti-cancer properties of Hepaclin-4 (Hep-4) extracts from the above mentioned plants. Methods: To evaluate the in vitro cytotoxicity of Hep-4, an ethanol extract of Hep-4 (500, 250, 125, 100, 50, and 10 μg/mL)L, doxorubicin, and 5-fluorouracil (5-FU) were assessed against the Hep G2 hepatocarcinoma cell line by monitoring proliferation using the MTT assay method. Results:The results demonstrated growth inhibition of cells in both dose-and time-dependent manners. Hep-4 inhibited Hep G2 transformation in 45% and the comparative group, 5-FU, effects on 40% of it. Conclusion: Our findings suggest that Hep-4 extracts have the potential to be a potent cytotoxic agent against Hep G2 cells, as it has commendable anti-proliferative effects against hepatocarcinoma cell lines and can be considered an effective adjuvant therapeutic agent.

show abstract

“…In Asian regions, HCC is the second most mortal cancer [ 7 ] with a peak incidence in East Asia [ 8 ]. Despite recent scientific advancement in hepatology, liver problems continue to increase [ 9 ]. There is no beneficial cure for this malignancy [ 10 ] and the recovery rate of HCC is low in most cases [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Promotion of HepG2 cell apoptosis by flower of Allium atroviolaceum and the mechanism of action

Khazaei

Hamid

Ramachandran

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundLiver cancer is a high incidence and fatal disease, the fifth most frequent cancer worldwide that is usually diagnosed at an advanced stage. The number of deaths from liver cancer has not declined even following various therapies. Plant secondary metabolites and their semi-synthetic derivatives play a principal role in anti-cancer drug therapy, since they are effective in the treatment of specific characteristics while also reducing side effects. Allium atroviolaceum, a plant of the genus Allium has been used in folk medicine to protect against several diseases. However, cytotoxicity and the anti-proliferative effect of Allium atroviolaceum remain unclear. This work aims to investigate the anticancer properties of Allium atroviolaceum and the mechanism of action.MethodsTo evaluate the in vitro cytotoxicity of flower of Allium atroviolaceum, methanol extract at a dose range from 100 to 3.12 μg/ml was assessed against the HepG2 hepatocarcinoma cell line, and also on normal 3T3 cells, by monitoring proliferation using the MTT assay method. A microscopy study was undertaken to observe morphological changes of HepG2 cells after treatment and cell cycle arrest and apoptosis were studied using flow cytometry. The apoptosis mechanism of action was assessed by the level of caspase-3 activity and expression of apoptosis related genes, Bcl-2, Cdk1 and p53. The combination effect of the methanolic extract with doxorubicin was also investigated by determination of a combination index.ResultsThe results demonstrated growth inhibition of cells in both dose- and time-dependent manners, while no cytotoxic effect on normal cell 3T3 was found. The results revealed the occurrence of apoptosis, illustrated by sub-G0 cell cycle arrest, the change in morphological feature and annexin-V and propidium iodide staining, which is correlated with Bcl-2 downregulation and caspase-3 activity, but p53-independent. In addition, a combination of Allium atroviolaceum and doxorubicin led to a significant synergistic effect.ConclusionThese findings suggest that Allium atroviolaceum flower extract has potential as a potent cytotoxic agent against HepG2 cell lines, as it has commendable anti-proliferative activities against human hepatocarcinoma and it can be considered as an effective adjuvant therapeutic agent after the clinical trials.

show abstract

In vivo simulated in vitro model of Jasminum sambac (Linn.) using mammalian liver slice technique

Cited by 15 publications

References 22 publications

Jasminum sambac: A Potential Candidate for Drug Development to Cure Cardiovascular Ailments

Jasminum sambac: A Potential Candidate for Drug Development to Cure Cardiovascular Ailments

Effects of Hepaclin-4 on Hepatocellular Carcinoma (Hep G2)

Promotion of HepG2 cell apoptosis by flower of Allium atroviolaceum and the mechanism of action

Contact Info

Product

Resources

About