In Vivo Psychopharmacological Investigation of Delphinium Denudatum and Amaranthus Spinosus Extracts on Wistar Rats

Abstract

Introduction:In our modern era, life style of human being changes and updates every day that may affect their health status. There is an incredible expectation that natural origin drugs lack undesirable effects not like synthetic drugs, though have the same potency and efficacy. No scientific data is available about the antianxiety properties of Delphinium denudatum root and Amaranthus spinosus leaves. In this regard, the present study was designed to carry out comparative and combined study on antianxiety properties of Delphinium denudatum root and Amaranthus spinosus leaves.Methods:Delphinium denudatum root and Amaranthus spinosus leaves were defatted with petroleum ether, and then extracted with hydroalcoholic solvent by soxhlation process. The hydroalcoholic extract of both drugs singly and in combination were evaluated for their anxiolytic effects on Wistar albino rats in doses of 200 and 400 mg/kg using different anti-anxiety tests like Elevated Plus Maze, Staircase, Actophotometer, and Light and Dark tests.Results:Both hydroalcoholic extracts possessed flavonoids, alkaloids, saponins, tannins, carbohydrates, proteins, amino acids, carbohydrates, steroids, sterols, etc. In the dose dependent manner, both the hydroalcoholic extracts produced good anxiolytic activity. The best result was obtained by a combination of them in higher dose.Conclusion:Hydroalcoholic extracts of Delphinium denudatum root and Amaranthus spinosus leaves and their combination may act as a potent anxiolytic agents in rats. Amaranthus spinosus was found to be more effective than Delphinium denudatum.

Chemistry, biological activities and toxic effects of alkaloidal constituents of genus Delphinium - A mini review

“…The other activity of D. denudatum on CNS was observed in Abid et al They identified that hydroalcoholic isolates extracted from D. denudatum root and Amaranthus spinosus leaves could have potential antianxiety activities in experimental rats. However, the results showed that A. spinosus was more potent than D. denudatum (73). Some studies have shown that D. denudatum extracts may act against morphineinduced tolerance and dependence symptoms via reducing morphine withdrawal syndrome signs could be an alternative therapy for morphine de-addiction (74)(75)(76).…”

Amaranthaceae plants of Israel and Palestine: Medicinal activities and unique compounds

“…Extract had antianxiety activity which was induced in rats by several methods. 323 An earlier study by the same group, where whole plant was extracted with methanol and extract was tested for antidepressant activity, that was induced in rats by several methods. In both studies, acute toxicity and general chemical composition were presented.…”

Bioactive Alkaloids from Plants Poisonous to Livestock in North America

“…Structure‐function studies of norditerpenoid alkaloids have shown that the aromatic ester functional group on MLA is a significant haptophore, and that the succinimide group imparts significant toxicity to the alkaloids ,. Current research interest in larkspur alkaloids includes the identification of new alkaloids, [24,25], species alkaloid composition, risk assessment, and the study of biological activities in rats and insects ,…”