In vivo photoacoustic guidance of stem cell injection and delivery for regenerative spinal cord therapies

Kubelick, Kelsey P.; Emelianov, Stanislav

doi:10.1117/1.nph.7.3.030501

Cited by 14 publications

(12 citation statements)

References 31 publications

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“…High-field magnetic resonance imaging has been used to detect oxygen metabolism in the brains of stroke patients and animal models [67][68][69][70]. Recent MSOT studies in animal models have demonstrated spinal cord hypoxia in experimental autoimmune encephalomyelitis (EAE) models [71] and white matter loss in a spinal cord injury model [72] and enabled the monitoring of image-guided stem cell therapy [73][74][75][76]. A recent study showed the feasibility of MSOT imaging of hyperoxia in the femur BM of a leukemia animal model [77].…”

Section: Discussionmentioning

confidence: 99%

Imaging increased metabolism in the spinal cord in mice after middle cerebral artery occlusion

Straumann

Fazio

et al. 2022

Preprint

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Emerging evidence indicates crosstalk between the brain and the hematopoietic system follow-ing cerebral ischemia. Here, we investigated metabolism and oxygenation in the spleen and spinal cord in a transient middle cerebral artery occlusion (tMCAO) mouse model that is wide-ly used in focal cerebral ischemia research. Naive, sham and tMCAO mice underwent positron emission tomography (PET) using [18F]fluorodeoxyglucose (FDG) for assessing glucose me-tabolism and multispectral optoacoustic tomography (MSOT) assisted with quantitative model-based reconstruction and unmixing algorithms for accurate mapping of oxygenation patterns in the peripheral tissues at 24 h after reperfusion. We found increased levels of [18F]FDG uptake and reduced MSOT oxygen saturation, indicating hypoxia in the thoracic spinal cord of tMCAO mice compared with sham-operated mice but not in the spleen. A positive correlation was observed between splenic and ipsilateral striatal [18F]FDG uptake. Reduced spleen size was observed in tMCAO mice compared with sham-operated mice ex vivo. tMCAO led to a significant increase in the numbers of mature T cells (CD4 and CD8) in femoral bone marrow tissues, concomitant with a stark reduction in these cell subsets in the spleen and their decrease in periph-eral blood. The numbers of mature granulocytes (determined as CD11b+Gr1hi cells) decreased in bone marrow tissues and blood but increased in the spleen. The combination of quantitative PET and MSOT thus enabled the observation of hypoxia and increased metabolic activity in the spinal cord of tMCAO mice at 24 h after occlusion compared to sham-operated mice.

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Section: Discussionmentioning

confidence: 99%

Imaging increased metabolism in the spinal cord in mice after middle cerebral artery occlusion

Straumann

Fazio

et al. 2022

Preprint

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“…Several nanocomposites have already been developed for MPA imaging, which requires optical and magnetic contrast. Examples include iron/gold core-shell nanoparticles 64 , 65 , liposomes loaded with gold nanoparticles and iron oxide nanoparticles 34 , 36 , gold nanoparticles decorated with SPIONs 66 , and Prussian blue nanocubes 67 - 69 .…”

Section: Synthesis Of Superparamagnetic Nanoparticles (Spions)mentioning

confidence: 99%

Magnetic particles in motion: magneto-motive imaging and sensing

Kubelick¹,

Mehrmohammadi²

2022

Theranostics

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Superparamagnetic nanoparticles have become an important tool in biomedicine. Their biocompatibility, controllable small size, and magnetic properties allow manipulation with an external magnetic field for a variety of diagnostic and therapeutic applications. Recently, the magnetically-induced motion of superparamagnetic nanoparticles has been investigated as a new source of imaging contrast. In magneto-motive imaging, an external, time-varying magnetic field is applied to move a magnetically labeled subject, such as labeled cells or tissue. Several major imaging modalities such as ultrasound, photoacoustic imaging, optical coherence tomography, and laser speckle tracking can utilize magneto-motive contrast to monitor biological events at smaller scales with enhanced contrast and sensitivity. In this review article, an overview of magneto-motive imaging techniques is presented, including synthesis of superparamagnetic nanoparticles, fundamental principles of magneto-motive force and its utility to excite labeled tissue within a viscoelastic medium, current capabilities of magneto-motive imaging modalities, and a discussion of the challenges and future outlook in the magneto-motive imaging domain.

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“…To treat nerve tissue injuries of the CNS, current research involves a variety of different aspects and techniques of cell therapy and tissue engineering. To monitor therapy progress and functional recovery or guide stem cell injection to the injury site, cells or applied biomaterials can be labeled with IONPs and thereby be monitored by several imaging modalities, such as MRI or microwave and optical imaging, among others [432][433][434][435][436][437][438][439][440][441]. The use of biomaterials can promote regeneration by providing structuring fiber scaffolds or ECM-like matrices to regenerate axons [442].…”

Section: Cnsmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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In vivo photoacoustic guidance of stem cell injection and delivery for regenerative spinal cord therapies

Cited by 14 publications

References 31 publications

Imaging increased metabolism in the spinal cord in mice after middle cerebral artery occlusion

Imaging increased metabolism in the spinal cord in mice after middle cerebral artery occlusion

Magnetic particles in motion: magneto-motive imaging and sensing

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

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