In vivo osteointegration of three-dimensional crosslinked gelatin-coated hydroxyapatite foams

Parathyroid hormone-related protein (107-111) improves the bone regeneration potential of gelatin–glutaraldehyde biopolymer-coated hydroxyapatite

“…The HA Glu scaffolds have been shown to be stable for about 2 weeks, but progressive degradation occurs thereafter [11]. Thus, and consistent with previous observations in a rabbit model [12], we found here, using lCT and histological analysis, scarce HA Glu material (although still detectable) in the bone defect area in our rat model at 4 weeks after implantation. Most proposed release strategies (i.e.…”

“…The biological performance of HA Glu scaffolds allows their definition as third-generation materials for orthopaedic use [39]. It was recently found that these scaffolds display excellent osteointegration properties when implanted into a cavitary bone defect in the rabbit epiphysis; thus, complete bone healing was observed at 4 months after implantation [12]. In the present study, we examined the performance of both types of implants tested -with and without PTHrP (107-111) -in a rat cortical bone defect at 4 weeks, which is an insufficient time period for complete bone healing [40].…”

“…Previous characterization of HA Glu foamy scaffolds indicate that they fulfil these criteria [11,12]. In fact, the nanocrystalline structure of HA Glu scaffolds, which is similar to that of native HA in bone, was found to promote bone formation, avoiding the generation of fibrous tissue around the implant [12]. The biological performance of HA Glu scaffolds allows their definition as third-generation materials for orthopaedic use [39].…”

“…This prompted us to evaluate whether loading PTHrP (107-111) into HA Glu scaffolds would provide a more appropriate biomaterial as an implant for improving new bone formation. These scaffolds were therefore implanted into a cortical defect in the rat tibial metaphysis, in which bone regeneration is known to proceed through intramembranous ossification [12]. Using this approach, we demonstrate that PTHrP (107-111)-containing HA Glu scaffolds display a clear advantage over peptide-unloaded scaffolds in promoting bone healing, as assessed by bone structure and histology, as well as molecular criteria.…”

“…Recently, three-dimensional HA foams with a high degree of porosity were prepared by sol-gel routes, and coated with gelatin-glutaraldehyde biopolymers to give them flexibility and easy manipulation in orthopaedic applications [9][10][11]. These materials were shown to allow cellular colonization and excellent biocompatibility both in vitro and in vivo [11,12].…”