In vivo osteoinductivity of gelatin β-tri-calcium phosphate sponge and bone morphogenetic protein-2 on an equine third metacarpal bone defect

Tsuzuki, Nao; Otsuka, Kuniko; Seo, Jong-pil; Yamada, Kazutaka; Haneda, Shingo; Furuoka, Hidefumi; Tabata, Yasuhiko; Sasaki, Naoki

doi:10.1016/j.rvsc.2011.12.002

Cited by 23 publications

(17 citation statements)

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“…It is defined that DDS is a system that administers a drug by enhancing its absorption and prolonging its activity by sustaining delivery at a fixed rate over a set time period and precisely targeting a specific area within the body23. Collagen and gelatin have been used as biodegradable carriers for DDS for administration of rhBMP-224.…”

Section: Discussionmentioning

confidence: 99%

Bone regeneration by polyhedral microcrystals from silkworm virus

Matsumoto

Ueda

Shimoyama

et al. 2012

Sci Rep

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Bombyx mori cypovirus is a major pathogen which causes significant losses in silkworm cocoon harvests because the virus particles are embedded in micrometer-sized protein crystals called polyhedra and can remain infectious in harsh environmental conditions for years. But the remarkable stability of polyhedra can be applied on slow-release carriers of cytokines for tissue engineering. Here we show the complete healing in critical-sized bone defects by bone morphogenetic protein-2 (BMP-2) encapsulated polyhedra. Although absorbable collagen sponge (ACS) safely and effectively delivers recombinant human BMP-2 (rhBMP-2) into healing tissue, the current therapeutic regimens release rhBMP-2 at an initially high rate after which the rate declines rapidly. ACS impregnated with BMP-2 polyhedra had enough osteogenic activity to promote complete healing in critical-sized bone defects, but ACS with a high dose of rhBMP-2 showed incomplete bone healing, indicating that polyhedral microcrystals containing BMP-2 promise to advance the state of the art of bone healing.

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Section: Discussionmentioning

confidence: 99%

Bone regeneration by polyhedral microcrystals from silkworm virus

Matsumoto

Ueda

Shimoyama

et al. 2012

Sci Rep

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“…Tsuzuki et al (2012) demonstrated that rhBMP-2/b-TCP sponges could significantly enhance bone regeneration as compared to either component alone. Moreover, significant improvements in the healing of surgically induced bone and osteochondral defects were demonstrated following treatment with rhBMP-2/b-TCP sponges in combination with MSCs (Seo et al 2014), or with MSCs and platelet rich plasma , Seo et al…”

Section: Discussionmentioning

confidence: 96%

Effect of recombinant human bone morphogenetic protein 2 (rhBMP-2) on equine bone formation: A preliminary in vitro and in vivo evaluation

Jackson

Fürst

Koch

et al. 2017

PHK

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Summary: Bone morphogenetic protein 2 (BMP-2) is a potent inducer of osteoblast-mediated bone formation and is used in orthopaedic applications in humans. This study was performed to evaluate (1) recombinant human BMP-2 (rhBMP-2) in clinical cases of subchondral cystic lesion (SCL) in horses and (2) the osteoinductive effects of rhBMP-2 treatment in vitro on primary equine osteoblasts. Three horses with SCLs of the proximal interphalangeal (PIP) joint and associated lameness were treated by transcortical debridement and implantation of a collagen sponge containing rhBMP-2. In the in vitro studies, equine osteoblasts were cultured with rhBMP-2 to assess the effects of rhBMP-2 on their differentiation and mineralization. The treatment of SCLs with rhBMP-2 was well tolerated by all the horses, and improvements in subchondral bone radiographic appearance and lameness score were evident between 7 and 10 months post-surgery. Equine osteoblasts treated with rhBMP-2 demonstrated enhanced mineralization as evidenced by increases in Alizarin Red S staining. This was accompanied by significant increases in alkaline phosphatase (ALP) expression and activity, and a significant decrease in secreted osteocalcin levels. In conclusion rhBMP-2 can be used to assist in subchondral bone repair in horses, and we provide in vitro evidence to suggest that its influence on bone formation is mediated through enhanced osteoblast activity.

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“…Mesenchymal stem cells (MSCs) are gaining increasing attention for osteochondral regeneration because of their ability to differentiate into several cell lineages including cartilage and bone cells (Diaz-Flores et al, 2012;Tsuzuki et al, 2012;Seo et al, 2013). Many growth factors have been used for osteochondral regeneration, such as bone morphogenetic protein (BMP-2), transforming growth factor-b1 (TGF-b1), and platelet rich plasma (PRP) (Sun et al, 2010;Chen et al, 2012;Nukavarapu and Dorcemus, 2013;Seo et al, 2013;Tsuzuki et al, 2013).…”

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confidence: 99%

“…Many growth factors have been used for osteochondral regeneration, such as bone morphogenetic protein (BMP-2), transforming growth factor-b1 (TGF-b1), and platelet rich plasma (PRP) (Sun et al, 2010;Chen et al, 2012;Nukavarapu and Dorcemus, 2013;Seo et al, 2013;Tsuzuki et al, 2013). Our previous studies demonstrated that gelatin/β-tricalcium phosphate (GT) sponge was useful for delivering MSCs and growth factors in osteochondral TE Tsuzuki et al, 2012Tsuzuki et al, , 2013.…”

mentioning

confidence: 99%