In vivo osteogenic differentiation of human turbinate mesenchymal stem cells in an injectable in situ-forming hydrogel

Kwon, Jin Seon; Kim, Sung Won; Kwon, Doo Yeon; Park, Seung Hun; Son, A Reum; Kim, Jae Ho; Kim, Moon Suk

doi:10.1016/j.biomaterials.2014.03.045

Cited by 61 publications

(46 citation statements)

References 31 publications

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“…Current studies on stem cells are mostly focused on their behaviors on the top of 2D platforms or within 3D hydrogels7828293031323334. While some experimental techniques like hanging drop method has interesting implications that the growth position may influence cellular functions of stem cells, it is still not clear whether the specific fate of stem cells is able to be harnessed by their culture position35.…”

Section: Discussionmentioning

confidence: 99%

Controlled Heterogeneous Stem Cell Differentiation on a Shape Memory Hydrogel Surface

Han

Bai

Liu

2014

Sci Rep

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The success of stem cell therapies is highly dependent on the ability to control their programmed differentiation. So far, it is commonly believed that the differentiation behavior of stem cells is supposed to be identical when they are cultured on the same homogeneous platform. However, in this report, we show that this is not always true. By utilizing a double-ion-triggered shape memory effect, the pre-seeded hMSCs were controllably located in different growth positions. Here, we demonstrate for the first time that the differentiation behavior of hMSCs is highly sensitive to their growth position on a hydrogel scaffold. This work will not only enrich the mechanisms for controlling the differentiation of stem cells, but also offer a one-of-a-kind platform to achieve a heterogeneously differentiated stem cell-seeded hydrogel scaffold for complex biological applications.

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Section: Discussionmentioning

confidence: 99%

Controlled Heterogeneous Stem Cell Differentiation on a Shape Memory Hydrogel Surface

Han

Bai

Liu

2014

Sci Rep

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“…Since the discovery of iPSCs by Takahashi et al [5], many researchers have explored the possibilities of self-renewal and regeneration of iPSC, embryonic stem cells, and adult stem cells, using TMSC technology [6][7][8]. Indeed, Takahashi et al not only introduced the iPSC technique but also neural differentiation in their study [5].…”

Section: Discussionmentioning

confidence: 99%

Standardizing Harvesting Methods for Human Turbinate Mesenchymal Stem Cells from Surgical Tissue

Kim¹,

Kim²,

Kim³

et al. 2017

Single Cell Biol

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In accordance with many disease occurrences, stem cell research has become an important study. Mesenchymal stem cell (MSC) is one of representative materials of stem cell research. MSCs are multipotent progenitor cells that can differentiate into various cell types including osteocytes, adipocytes, chondrocytes, myocytes, stromal cells and neurons. MSCs have been derived from bone marrow, umbilical cord, umbilical cord blood, fat, and other body organs. Among them, human inferior turbinate and nasal septal cartilages are good materials for extraction of MSCs. For extraction of MSCs, we used MSC extraction protocol from ARCO sensorineural laboratory. In order to identified extracted MSCs, Immunofluorescence to CD90 and CD73 (or CD105) cell-surface markers of MSCs was performed using the MSC phenotyping kit (Miltenyi Biotec, Germany). hMSCs derived from inferior turbinate and nasal septal cartilage were shown positive results. Further study, more studies need to comparative analysis of differentiated target cells from our MSCs.

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“…Copolymerization of the PEG framework with natural polymers, such as collagen or hyaluronic acid, can also improve its inert bioactivity [44, 45]. Furthermore, the copolymerization of PEG with certain synthetic biomaterials, such as poly(ε-caprolactone) (PCL), poly(lactic-co-glycolic acid) (PLGA), and poly( N -isopropylacrylamide), endows the copolymer with a thermo-sensitive transition feature and enhanced bioactivity, which is preferable for in situ soft tissue engineering [46–48]. …”

Section: Biomaterials Used As Injectable Scaffoldsmentioning

confidence: 99%

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

Chang

Ahuja

et al. 2017

Materials Science and Engineering: R: Reports

198

170

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Injectable scaffolds are appealing for tissue regeneration because they offer many advantages over pre-formed scaffolds. This article provides a comprehensive review of the injectable scaffolds currently being investigated for dental and craniofacial tissue regeneration. First, we provide an overview of injectable scaffolding materials, including natural, synthetic, and composite biomaterials. Next, we discuss a variety of characteristic parameters and gelation mechanisms of the injectable scaffolds. The advanced injectable scaffolding systems developed in recent years are then illustrated. Furthermore, we summarize the applications of the injectable scaffolds for the regeneration of dental and craniofacial tissues that include pulp, dentin, periodontal ligament, temporomandibular joint, and alveolar bone. Finally, our perspectives on the injectable scaffolds for dental and craniofacial tissue regeneration are offered as signposts for the future advancement of this field.

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In vivo osteogenic differentiation of human turbinate mesenchymal stem cells in an injectable in situ-forming hydrogel

Cited by 61 publications

References 31 publications

Controlled Heterogeneous Stem Cell Differentiation on a Shape Memory Hydrogel Surface

Controlled Heterogeneous Stem Cell Differentiation on a Shape Memory Hydrogel Surface

Standardizing Harvesting Methods for Human Turbinate Mesenchymal Stem Cells from Surgical Tissue

Injectable scaffolds: Preparation and application in dental and craniofacial regeneration

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