In vivo odorant input induces distinct synaptic plasticity of GABAergic synapses in developing zebrafish olfactory bulb

Hu, Bin; Jin, Chen; Zhang, Yi-Qian; Miao, Haoran; Wang, Feng

doi:10.1016/j.bbrc.2020.07.106

Cited by 2 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…23,24 Patch-clamp is often used to record rat OB neurons in vitro 25 and zebrafish OB neurons in vivo. 26 Patch-clamp recordings obtain steadystate whole-cell signals of the OB, providing vital information to the study of ion channels and synaptic connections of OB neurons. Long-term stable OB signals can be obtained by electrophysiological recording with electrodes, which is also often mentioned in some olfactory studies.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Many studies have focused on the recording of signals from the OB, which is a relay station for signal processing in the olfactory system. Optical calcium imaging and functional magnetic resonance imaging have extremely high resolution, do not cause damage to the OB tissue, and have been used to clarify the code of retronasal odor intensity. , Patch-clamp is often used to record rat OB neurons in vitro and zebrafish OB neurons in vivo . Patch-clamp recordings obtain steady-state whole-cell signals of the OB, providing vital information to the study of ion channels and synaptic connections of OB neurons.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In Vivo Bioelectronic Nose Based on a Bioengineered Rat Realizes the Detection and Classification of Multiodorants

Zhu

Liu

Tian

et al. 2022

ACS Chem. Neurosci.

View full text Add to dashboard Cite

Inspired by the powerful capability of the biological olfactory system, we developed an in vivo bioelectronic nose based on a bioengineered rat by recording electrophysiological-responsive signals from the olfactory bulb with implanted multichannel microelectrodes. The bioengineered rat was prepared by overexpressing a selected olfactory receptor (OR3) on the rat olfactory epithelium, and multichannel electrophysiological signals were obtained from the mitral/tufted (M/T) cell population of the olfactory bulb. The classification of target multiodorants was realized by analyzing the redundant stimuli-responsive firing information. Ligand odorants induced significant firing changes with specific response patterns compared with nonligand odorants. The responsive curves were dependent on the concentration of target ligand odorants ranging from 10–6 to 10–3 M, and the detection limit was as low as 10–5 M. In addition, different ligand odorants were successfully discriminated via principal component analysis. This in vivo bioelectronic nose provides a novel approach for the detection of specific target odorants and has promising application potential in the field of rapid on-site odor discrimination.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vivo Bioelectronic Nose Based on a Bioengineered Rat Realizes the Detection and Classification of Multiodorants

Zhu

Liu

Tian

et al. 2022

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…The activity of M/T cells is extensively modified by dynamic interactions with GABAergic and dopaminergic interneurons within the OB (Burton, 2017). These interneurons form both dendro-dendritic reciprocal synapses and axo-dendritic synapses with M/T cells and mediate lateral and recurrent inhibition onto M/T cells (Margrie et al, 2001;Aungst et al, 2003;Hu et al, 2017Hu et al, , 2020a), which play a major role in transforming odor representations (Nusser et al, 2001;McGann, 2013). Previous studies have shown that odor enrichment can induce response changes in the inhibitory interneurons of the OB (Mandairon et al, 2008) and that GABAergic inhibition onto M/T cells is crucial for pattern separation and odor discrimination (Abraham et al, 2010;Godde et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Improved Separation of Odor Responses in Granule Cells of the Olfactory Bulb During Odor Discrimination Learning

Wang

Chen

et al. 2020

Front. Cell. Neurosci.

View full text Add to dashboard Cite

In the olfactory bulb, olfactory information is translated into ensemble representations by mitral/tufted cells, and these representations change dynamically in a contextdependent manner. In particular, odor representations in mitral/tufted cells display pattern separation during odor discrimination learning. Although granule cells provide major inhibitory input to mitral/tufted cells and play an important role in pattern separation and olfactory learning, the dynamics of odor responses in granule cells during odor discrimination learning remain largely unknown. Here, we studied odor responses in granule cells of the olfactory bulb using fiber photometry recordings in awake behaving mice. We found that odors evoked reliable, excitatory responses in the granule cell population. Intriguingly, during odor discrimination learning, odor responses in granule cells exhibited improved separation and contained information about odor value. In conclusion, we show that granule cells in the olfactory bulb display learning-related plasticity, suggesting that they may mediate pattern separation in mitral/tufted cells.

show abstract

In vivo odorant input induces distinct synaptic plasticity of GABAergic synapses in developing zebrafish olfactory bulb

Cited by 2 publications

References 37 publications

In Vivo Bioelectronic Nose Based on a Bioengineered Rat Realizes the Detection and Classification of Multiodorants

In Vivo Bioelectronic Nose Based on a Bioengineered Rat Realizes the Detection and Classification of Multiodorants

Improved Separation of Odor Responses in Granule Cells of the Olfactory Bulb During Odor Discrimination Learning

Contact Info

Product

Resources

About