In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe

Chen, Xiaoqiang; Lee, Da-Young; Yu, Sungsook; Kim, Gyoungmi; Lee, Song Yi; Cho, Yejin; Jeong, Haengdueng; Nam, Ki Taek; Yoon, Juyoung

doi:10.1016/j.biomaterials.2017.01.020

Cited by 97 publications

(48 citation statements)

References 35 publications

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“…For the purpose to explore the LMP occurrence, the acridine orange (AO) probe was further utilized to monitor LMP of 4T1 cells (Figure h). When the 4T1 cells were treated with Mo 2 C‐PVA nanoflakes under laser illumination, the red fluorescence of lysosome decreased accompanying with the increased orange fluorescence in the whole cytoplasm, indicating that LMP was induced by PTT‐induced hyperemia and then lysosomotropic compounds released from lysosomal into the cytoplasm, which might be associated with an early event in cancer‐cell apoptosis . Similarly, during the evaluation, 4T1 cells incubated with Mo 2 C‐PVA nanoflakes or under laser irradiation alone exhibited negligible morphology changes in mitochondria (Figure i), actin cytoskeleton (Figure j), and ER (Figure k).…”

Section: Resultsmentioning

confidence: 87%

Ultrathin Molybdenum Carbide MXene with Fast Biodegradability for Highly Efficient Theory‐Oriented Photonic Tumor Hyperthermia

Feng

Wang

Zhou

et al. 2019

Adv Funct Materials

177

109

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The explosion of emerging high-performance 2D MXenes in theranostic nanomedicine is still at the preliminary stage. Despite tremendous efforts devoted to photonic tumor hyperthermia, current photothermal-conversion nanoagents still suffer from critical issues preventing further clinical translation such as low biodegradability. Here, for the first time, the construction of novel 2D molybdenum carbide (Mo 2 C) MXenes for photonic tumor hyperthermia is reported. The structure of both bulk Mo 2 Ga 2 C ceramic and Mo 2 C MXene is fully revealed. Especially, computational simulation, as a novel strategy and a powerful tool for photonic-performance prediction, is employed to reveal that Mo 2 C MXene is featured with intense near-infrared (NIR) absorption, covering the first and the second biological transparency window (NIR I and II). After further surface engineering with polyvinyl alcohol (PVA), Mo 2 C-PVA nanoflakes exhibit high biocompatibility and fast degradability. Importantly, it is experimentally corroborated that Mo 2 C-PVA nanoflakes possess intriguing broad absorption band spanning NIR in both the I and II regions, and desirable photothermal-conversion efficiency (24.5% for NIR I and 43.3% for NIR II). This study not only broadens the nanomedical applications of MXene by fabricating novel material members (Mo 2 C), but also provides the paradigm of inorganic multifunctional biomedical nanoplatform with desirable biodegradability and high therapeutic performance.

show abstract

Section: Resultsmentioning

confidence: 87%

Ultrathin Molybdenum Carbide MXene with Fast Biodegradability for Highly Efficient Theory‐Oriented Photonic Tumor Hyperthermia

Feng

Wang

Zhou

et al. 2019

Adv Funct Materials

177

109

View full text Add to dashboard Cite

show abstract

“…For example, Chen et al (2017) synthetised a nearinfrared fluorescent probe that is cleaved by cathepsin B, which is highly expressed in tumour cells, and can subsequently be excited with near-infrared light to generate a cytotoxic agent. The authors tested their concept in vivo in subcutaneous tumours and showed that their photodynamic therapeutics approach prevented tumour growth without affecting normal tissues.…”

Section: Future Applications and Combined Imaging Modesmentioning

confidence: 99%

Molecular mobility and activity in an intravital imaging setting – implications for cancer progression and targeting

Nobis

Warren

Lucas

et al. 2018

Journal of Cell Science

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Molecular mobility, localisation and spatiotemporal activity are at the core of cell biological processes and deregulation of these dynamic events can underpin disease development and progression. Recent advances in intravital imaging techniques in mice are providing new avenues to study real-time molecular behaviour in intact tissues within a live organism and to gain exciting insights into the intricate regulation of live cell biology at the microscale level. The monitoring of fluorescently labelled proteins and agents can be combined with autofluorescent properties of the microenvironment to provide a comprehensive snapshot of in vivo cell biology. In this Review, we summarise recent intravital microscopy approaches in mice, in processes ranging from normal development and homeostasis to disease progression and treatment in cancer, where we emphasise the utility of intravital imaging to observe dynamic and transient events in vivo. We also highlight the recent integration of advanced subcellular imaging techniques into the intravital imaging pipeline, which can provide in-depth biological information beyond the singlecell level. We conclude with an outlook of ongoing developments in intravital microscopy towards imaging in humans, as well as provide an overview of the challenges the intravital imaging community currently faces and outline potential ways for overcoming these hurdles.

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“…NPs with activatable fluorescence have also been designed to target tumor‐specific molecules. This is the case of the polymeric NPs designed by Chen et al, composed of two cyanine dye moieties liked by a cathepsin B‐activated peptide encapsulated in a polymeric matrix . Cathepsin B, a lysosomal protease highly upregulated in tumors, cleaves the peptide linker activating the NP fluorescence, which is quenched due to resonant energy transfer (RET) between the two cyanine moieties when they are linked.…”

Section: Nanomaterials For Fluorescence‐guided Theranostics: Single‐mmentioning

confidence: 99%

Beyond Phototherapy: Recent Advances in Multifunctional Fluorescent Nanoparticles for Light‐Triggered Tumor Theranostics

Rosal

Jia

Jaque

2018

Adv Funct Materials

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Nanoparticles (NPs) have attracted great attention for cancer diagnosis and therapy thanks to their unique properties that enable specific tumor uptake, image-guided diagnosis, and tumor suppression triggered by one or more therapeutic modalities. Light-activated NPs have gained particular interest as minimally invasive theranostic agents for fluorescence-guided tumor diagnosis and highly selective phototherapy. The most recent advances in the field focus on shifting their spectral operation range to the second biological window (1000-1400 nm), while improving their efficacy, selectivity, and multifunctionality. In the past few years, multiple systems capable of light-triggered tumor diagnosis through fluorescence imaging (and in many cases, other imaging modalities) and subsequent tumor therapy have been synthesized and evaluated in small animal models. This review summarizes the different approaches for in vivo tumor theranostics, highlighting their advantages and limitations, and provides a critical analysis of the current state of the field.

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In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe

Cited by 97 publications

References 35 publications

Ultrathin Molybdenum Carbide MXene with Fast Biodegradability for Highly Efficient Theory‐Oriented Photonic Tumor Hyperthermia

Ultrathin Molybdenum Carbide MXene with Fast Biodegradability for Highly Efficient Theory‐Oriented Photonic Tumor Hyperthermia

Molecular mobility and activity in an intravital imaging setting – implications for cancer progression and targeting

Beyond Phototherapy: Recent Advances in Multifunctional Fluorescent Nanoparticles for Light‐Triggered Tumor Theranostics

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