In vivo MRI evaluation of anterograde manganese transport along the visual pathway following whole eye transplantation

Komatsu, Chiaki; Merwe, Yolandi van der; He, Lin; Kasi, Anisha; Sims, Jeffrey R.; Miller, Maxine R.; Rosner, Ian; Khatter, Neil J.; Su, An-Jey A.; Schuman, Joel S.; Washington, Kia M.; Chan, Kevin C.

doi:10.1016/j.jneumeth.2022.109534

Cited by 5 publications

(9 citation statements)

References 57 publications

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“…18,20 While transplantation of surrounding tissue could theoretically pose a threat to the viability of the eye by limiting space for postoperative edema, if successful, this may serve as a proof of concept for WET to occur concomitantly with FT in the future. 19,22 Moreover, in unilateral ocular and periorbital tissue transplantation, authors did not identify any adverse structural or functional effects in the native, contralateral eye, contrary to commonly reported sympathetic ophthalmia after eye trauma. 45 In summary, our results suggest that ocular allograft perfusion and survival are likely with current technological and surgical advancements.…”

Section: Discussionmentioning

confidence: 92%

“… 15 , 23 While previous studies performed WET alone, recent studies have included soft tissue and skeletal components associated with the donor eye allograft, including the bony orbit, the frontal bone, eyelids, and ears. 18 – 20 , 22 , 23 All arterial anastomoses were made end-to-end except for Davidson et al, who utilized human cadaveric vein grafts for arterial anastomotic connections between the vessels of the allograft and recipient. 9 The surgical outcomes in mammalian models are presented in Table 6 .…”

Section: Resultsmentioning

confidence: 99%

“…Fourteen articles utilized mammalian and cadaveric models. 2,[11][12][13][14][15][16][17][18][19][20][21][22][23] The remaining 19 utilized cold-blooded species. [3][4][5][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] After data extraction, a total of 1998 eyes were included, 1831 of which belonged to nonmammalian organisms and 167 to mammals, including 13 human cadavers and two living humans.…”

Section: Resultsmentioning

confidence: 99%

“… 52 In vivo MRI studies of murine WET found that anterograde transport remained possible after intravitreal ion injection, but retrograde transport halted at the coaptation site. 22 Hence, coaptation was incapable of functionally connecting the RGCs to the recipient’s CNS. These findings align with the current literature indicating that the CNS is less likely to regenerate even with proper nervous coaptation.…”

Section: Discussionmentioning

confidence: 99%

“… 53 Similarly, peripheral nerve explants grafted into the vitreous body of the rodent eye promoted RGC survival in resected ONs. 54 Although studies collected in our review discuss the potential use of peripheral nerve grafting in WET, 22 this has yet to be implemented in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Technical Feasibility of Whole-eye Vascular Composite Allotransplantation: A Systematic Review

Laspro

Chaya

Brydges

et al. 2023

Plastic and Reconstructive Surgery - Global Open

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Background: There are over 43 million individuals in the world who are blind. As retinal ganglion cells are incapable of regeneration, treatment modalities for this condition are limited. Since first incepted in 1885, whole-eye transplantation (WET) has been proposed as the ultimate cure for blindness. As the field evolves, different aspects of the surgery have been individually explored, including allograft viability, retinal survival, and optic nerve regeneration. Due to the paucity in the WET literature, we aimed to systematically review proposed WET surgical techniques to assess surgical feasibility. Additionally, we hope to identify barriers to future clinical application and potential ethical concerns that could be raised with surgery. Methods: We conducted a systematic review of PubMed, Embase, Cochrane Library, and Scopus from inception to June 10, 2022, to identify articles pertaining to WET. Data collection included model organisms studied, surgical techniques utilized, and postoperative functional outcomes. Results: Our results yielded 33 articles, including 14 mammalian and 19 cold-blooded models. In studies performing microvascular anastomosis in mammals, 96% of allografts survived after surgery. With nervous coaptation, 82.9% of retinas had positive electroretinogram signals after surgery, indicating functional retinal cells after transplantation. Results on optic nerve function were inconclusive. Ocular-motor functionality was rarely addressed. Conclusions: Regarding allograft survival, WET appears feasible with no complications to the recipient recorded in previous literature. Functional restoration is potentially achievable with a demonstrated positive retinal survival in live models. Nevertheless, the potential of optic nerve regeneration remains undetermined.

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Section: Discussionmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Technical Feasibility of Whole-eye Vascular Composite Allotransplantation: A Systematic Review

Laspro

Chaya

Brydges

et al. 2023

Plastic and Reconstructive Surgery - Global Open

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Whole Eye Transplant—The Journey Begins

Bradley,

Woog

2024

JAMA Ophthalmol

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Improved reconstruction of crossing fibers in the mouse optic pathways with orientation distribution function fingerprinting

Filipiak,

Sajitha,

Shepherd

et al. 2023

Magnetic Resonance in Med

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PurposeThe accuracy of diffusion MRI tractography reconstruction decreases in the white matter regions with crossing fibers. The optic pathways in rodents provide a challenging structure to test new diffusion tractography approaches because of the small crossing volume within the optic chiasm and the unbalanced 9:1 proportion between the contra‐ and ipsilateral neural projections from the retina to the lateral geniculate nucleus, respectively.MethodsCommon approaches based on Orientation Distribution Function (ODF) peak finding or statistical inference were compared qualitatively and quantitatively to ODF Fingerprinting (ODF‐FP) for reconstruction of crossing fibers within the optic chiasm using in vivo diffusion MRI ( healthy C57BL/6 mice). Manganese‐Enhanced MRI (MEMRI) was obtained after intravitreal injection of manganese chloride and used as a reference standard for the optic pathway anatomy.ResultsODF‐FP outperformed by over 100% all the tested methods in terms of the ratios between the contra‐ and ipsilateral segments of the reconstructed optic pathways as well as the spatial overlap between tractography and MEMRI.ConclusionIn this challenging model system, ODF‐Fingerprinting reduced uncertainty of diffusion tractography for complex structural formations of fiber bundles.

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In vivo MRI evaluation of anterograde manganese transport along the visual pathway following whole eye transplantation

Cited by 5 publications

References 57 publications

Technical Feasibility of Whole-eye Vascular Composite Allotransplantation: A Systematic Review

Technical Feasibility of Whole-eye Vascular Composite Allotransplantation: A Systematic Review

Whole Eye Transplant—The Journey Begins

Improved reconstruction of crossing fibers in the mouse optic pathways with orientation distribution function fingerprinting

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