In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography

Koenig, Anne; Hervé, Lionel; Josserand, Véronique; Berger, Michel; Boutet, J.; Silva, Anabela Da; Dinten, Jean‐Marc; Peltié, Philippe; Coll, Jean‐Luc; Rizo, P.

doi:10.1117/1.2884505

Cited by 58 publications

(50 citation statements)

References 17 publications

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“…Specifically, CW FDOT is based on the measurement of the attenuation of a steady-state excitation light. [1][2][3][4][5] Frequency-domain FDOT is based on the measurement of the phase and demodulation of an amplitude-modulated excitation. 6,7 TR FDOT is based on the temporal measurement of the distortion of an excitation light pulse.…”

Section: Introductionmentioning

confidence: 99%

A time‐domain wavelet‐based approach for fluorescence diffuse optical tomography

et al. 2010

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Purpose: In the context of fluorescence diffuse optical tomography, determining the optimal way to exploit the time-resolved information has been receiving much attention and different features of the time-resolved signals have been introduced. In this article, the authors revisit and generalize the notion of feature, considering the projection of the measurements onto some basis functions. This leads the authors to propose a novel approach based on the wavelet transform of the measurements. Methods: A comparative study between the reconstructions obtained from the proposed waveletbased approach and the reconstructions obtained from the reference temporal moments is provided. An inhomogeneous cubic medium is considered. Reconstructions are performed from synthetic measurements assuming Poisson noise statistics. In order to provide fairly comparable reconstructions, the reconstruction scheme is associated with a particular procedure for selecting the regularization parameter. Results: In the noise-free case, the reconstruction quality is shown to be mainly driven by the number of selected features. In the presence of noise, however, the reconstruction quality depends on the type of the features. In this case, the wavelet approach is shown to outperform the moment approach. While the optimal time-resolved reconstruction quality, which is obtained considering the whole set of time samples, is recovered using only eight wavelet functions, it cannot be attained using moments. It is finally observed that the time-resolved information is of limited utility, in terms of reconstruction, when the maximum number of detected photons is lower than 10 5 . Conclusions: The wavelet approach allows for better exploiting the time-resolved information, especially when the number of detected photons is low. However, when the number of detected photons decreases below a certain threshold, the time-resolved information itself is shown to be of limited utility.

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Section: Introductionmentioning

confidence: 99%

A time‐domain wavelet‐based approach for fluorescence diffuse optical tomography

et al. 2010

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“…[25][26][27][28][29] Over the past decades, such optical imaging modalities have undergone rapid development and been used to monitor primary tumor growth and metastases, 30,31 tumor cell and enzyme activity, [32][33][34] tumor cell surface receptors, [35][36][37] apoptosis, 38 anti-tumor treatment effects 39 and biodistribution and accumulation of fluorescencelabeled molecules, such as therapeutic antibodies. [40][41][42][43] The majority of this macroscopic in vivo research was not correlated with microscopic ex vivo fluorescence histology analysis.…”

Section: Introductionmentioning

confidence: 99%

Improved decision making for prioritizing tumor targeting antibodies in human xenografts: Utility of fluorescence imaging to verify tumor target expression, antibody binding and optimization of dosage and application schedule

Dobosz¹,

Haupt²,

Scheuer³

2016

mAbs

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Preclinical efficacy studies of antibodies targeting a tumor-associated antigen are only justified when the expression of the relevant antigen has been demonstrated. Conventionally, antigen expression level is examined by immunohistochemistry of formalin-fixed paraffin-embedded tumor tissue section. This method represents the diagnostic "gold standard" for tumor target evaluation, but is affected by a number of factors, such as epitope masking and insufficient antigen retrieval. As a consequence, variances and discrepancies in histological staining results can occur, which may influence decision-making and therapeutic outcome. To overcome these problems, we have used different fluorescence-labeled therapeutic antibodies targeting human epidermal growth factor receptor (HER) family members and insulin-like growth factor-1 receptor (IGF1R) in combination with fluorescence imaging modalities to determine tumor antigen expression, drug-target interaction, and biodistribution and tumor saturation kinetics in non-small cell lung cancer xenografts. For this, whole-body fluorescence intensities of labeled antibodies, applied as a single compound or antibody mixture, were measured in Calu-1 and Calu-3 tumor-bearing mice, then ex vivo multispectral tumor tissue analysis at microscopic resolution was performed. With the aid of this simple and fast imaging method, we were able to analyze the tumor cell receptor status of HER1-3 and IGF1R, monitor the antibody-target interaction and evaluate the receptor binding sites of anti-HER2-targeting antibodies. Based on this, the most suitable tumor model, best therapeutic antibody, and optimal treatment dosage and application schedule was selected. Predictions drawn from obtained imaging data were in excellent concordance with outcome of conducted preclinical efficacy studies. Our results clearly demonstrate the great potential of combined in vivo and ex vivo fluorescence imaging for the preclinical development and characterization of monoclonal antibodies.

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“…Continuous-wave (CW) FDOT is based on the measurement of the attenuation of a steady state excitation light [9][10][11][12]. Frequency-domain (FD) FDOT is based on the measurement of the phase and demodulation of an amplitude-modulated excitation [13].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence diffuse optical tomography: Time-resolved versus continuous-wave in the reflectance configuration

Ducros

D’Andrea

Bassi

et al. 2011

IRBM

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International audienceContext and objectives In the context of fluorescence diffuse optical tomography, the time-resolved approach was shown to improve the reconstruction quality compared to the continuous-wave approach when adopting the transmittance configuration. However, the improvement seriously decreases in the presence of noise. The scope of this paper is to investigate the additional value of the time-resolved approach in the reflectance configuration. Material and methods A comparative study between time-resolved and continuous-wave reconstructions is provided in the reflectance configuration. Reconstructions are performed from synthetic measurements in a slab geometry assuming Poisson noise statistics. Results and discussion In the reflectance configuration, the reconstruction quality, expressed in terms of the global reconstruction error or in terms of the localization and quantification of a local inclusion, is shown to be sensibly higher when the time-resolved approach is considered rather than the continuous-wave one. This behavior is observed for which the maximum number of detected photons is around 106. Conclusion In the reflectance configuration, considering realistic level of signal the time-resolved approach is shown to outperform continuous-wave approach

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In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography

Cited by 58 publications

References 17 publications

A time‐domain wavelet‐based approach for fluorescence diffuse optical tomography

A time‐domain wavelet‐based approach for fluorescence diffuse optical tomography

Improved decision making for prioritizing tumor targeting antibodies in human xenografts: Utility of fluorescence imaging to verify tumor target expression, antibody binding and optimization of dosage and application schedule

Fluorescence diffuse optical tomography: Time-resolved versus continuous-wave in the reflectance configuration

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