In vivo measurement of local aortic pulse‐wave velocity in mice with MR microscopy at 17.6 tesla

Herold, Volker; Parczyk, Marco; Mörchel, Philipp; Ziener, Christian H.; Klug, Gert; Bauer, Wolfgang R.; Rommel, Eberhard; Jakob, Peter M.

doi:10.1002/mrm.21957

Cited by 41 publications

(51 citation statements)

References 37 publications

(47 reference statements)

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“…A customwritten platform in Matlab, based on the algorithm described by Rabben et al (2002), was used to reconstruct the complete Guo and Kassab, 2003;Guo et al, 2006;Herold et al, 2009;Luo et al, 2009;Reddy et al, 2003;Wagenseil et al, 2005;Wang, 2005;Williams et al, 2007). If local ex vivo pressure-diameter curves or distensibility values were reported, these were converted into local pulse wave velocities (PWV) using the Bramwell-Hill equation.…”

Section: Governing Equationsmentioning

confidence: 99%

A 1D model of the arterial circulation in mice

Aslanidou

Trachet

Reymond

et al. 2016

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SummaryAt a time of growing concern over the ethics of animal experimentation, mouse models are still an indispensable source of insight into the cardiovascular system and its most frequent pathologies. Nevertheless, reference data on the murine cardiovascular anatomy and physiology are lacking. In this work, we developed and validated an in silico, one dimensional model of the murine systemic arterial tree consisting of 85 arterial segments. Detailed aortic dimensions were obtained in vivo from contrast-enhanced micro-computed tomography in 3 male, C57BL/6J anesthetized mice and 3 male ApoE -/-mice, all 12-weeks old. Physiological input data were gathered from a wide range of literature data. The integrated form of the Navier-Stokes equations was solved numerically to yield pressures and flows throughout the arterial network. The resulting model predictions have been validated against invasive pressure waveforms and noninvasive velocity and diameter waveforms that were measured in vivo on an independent set of 47 mice. In conclusion, we present a validated one-dimensional model of the anesthetized murine cardiovascular system that can serve as a versatile tool in the field of preclinical cardiovascular research.

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Section: Governing Equationsmentioning

confidence: 99%

A 1D model of the arterial circulation in mice

Aslanidou

Trachet

Reymond

et al. 2016

ALTEX

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“…Therefore, we recently introduced an MRI method that allows the examination of local PWV. 9,10 In the present study, we applied this technique to monitor the natural progression of atherosclerosis in the murine aorta and correlated local PWV with vessel wall thickness in serial measurements. Both parameters were examined in vivo in wild-type (WT) control mice and apolipoprotein E-deficient (ApoE −/−…”

Section: Clinical Perspective On P 923mentioning

confidence: 99%

“…9,13 For the measurement of the time course of the parameters Q and A, a highresolution phase-contrast (PC) Cine-FLASH sequence was performed perpendicular to the aorta with through plane flow encoding (slices, 1; TE=1.7 ms; TR=5 ms; matrix, 150 × 150; pixel size, 147 × 147 × 1000 µm 3 ; frames per heart cycle, 40). By the use of an interlaced acquisition mode, a temporal resolution of 1 ms could be achieved.…”

Section: Mri Of the Local Pwvmentioning

confidence: 99%

Local Arterial Stiffening Assessed by MRI Precedes Atherosclerotic Plaque Formation

Gotschy

Bauer

Schrodt

et al. 2013

Circ: Cardiovascular Imaging

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) mice, which spontaneously develop atherosclerotic lesions of morphology similar to those observed in humans. 11,12 Local PWV and vessel wall thickness were evaluated by timeresolved flow and morphology measurement using ultrahighfield MR microscopy at 17.6 T. In addition, we performed histological examinations to investigate structural changes of the vessel wall at the time of imaging.Background-Atherosclerosis is known to impair vascular function and cause vascular stiffening. The aim of this study was to evaluate the potential predictive role of vascular stiffening in the early detection of atherosclerosis. Therefore, we investigated the time course of early functional and morphological alterations of the vessel wall in a murine atherosclerosis model. Because initial lesions are distributed inhomogeneously in early-stage atherosclerosis, MR microscopy was performed to measure vascular elasticity locally, specifically the local pulse wave velocity and the arterial wall thickness. Methods and Results-Local pulse wave velocity and the mean arterial wall thickness were determined in the ascending and the abdominal aortae of ApoE −/− and wild-type mice. In vivo MRI revealed that baseline pulse wave velocity and morphology were similar in 6-week-old ApoE −/− and WT mice, whereas at the age of 18 weeks, local pulse wave velocity was significantly elevated in ApoE −/− mice. Significantly increased vessel wall thickness was not found in ApoE −/− mice until the age of 30 weeks. Histological analysis of the aortae of ApoE −/− and WT mice showed that increased pulse wave velocity coincided with the fragmentation of the elastic laminae in the arterial wall, which is hypothesized to induce early vascular stiffening and may be promoted by macrophage-mediated matrix degradation. Conclusions-We

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“…The downtrades are greater field inhomogeneity and susceptibility to artifacts and higher radiofrequency (RF) power deposition in tissue-measured by the specific absorption rate (SAR) [101]. Overall, although some authors report nice image acquisitions at clinical field strength (1.5-3 T) with optimized coils [29,53,62], or at the very high field of 17.6 T [60,61], it is generally admitted that the range of 7-11.7 T offers the best trade off for most applications in small animals [48]. Systems can be either horizontally or vertically mounted.…”

Section: Technical Considerationsmentioning

confidence: 98%

High field magnetic resonance imaging of rodents in cardiovascular research

et al. 2016

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Transgenic and gene knockout rodent models are primordial to study pathophysiological processes in cardiovascular research. Over time, cardiac MRI has become a gold standard for in vivo evaluation of such models. Technical advances have led to the development of magnets with increasingly high field strength, allowing specific investigation of cardiac anatomy, global and regional function, viability, perfusion or vascular parameters. The aim of this report is to provide a review of the various sequences and techniques available to image mice on 7-11.7 T magnets and relevant to the clinical setting in humans. Specific technical aspects due to the rise of the magnetic field are also discussed.

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In vivo measurement of local aortic pulse‐wave velocity in mice with MR microscopy at 17.6 tesla

Cited by 41 publications

References 37 publications

A 1D model of the arterial circulation in mice

A 1D model of the arterial circulation in mice

Local Arterial Stiffening Assessed by MRI Precedes Atherosclerotic Plaque Formation

High field magnetic resonance imaging of rodents in cardiovascular research

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