In vivo magnetic resonance imaging of endogenous neuroblasts labelled with a ferumoxide–polycation complex

Panizzo, R; Kyrtatos, Panagiotis G.; Price, Anthony N.; Gadian, David G.; Ferretti, Patrizia; Lythgoe, Mark F.

doi:10.1016/j.neuroimage.2008.10.062

Cited by 44 publications

(46 citation statements)

References 35 publications

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“…It has been previously reported that cationic transfection agents might improve the efficiency of MNPs intracellular uptake [19,[26][27][28][29], especially in non-phagocytic cells. Thus, the ability of cationic compounds such as PL and PS might increase the capacity of VM hNSCs to internalize MNPs.…”

Section: Resultsmentioning

confidence: 99%

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Ramos-Gómez¹,

Seiz²,

Martínez‐Serrano³

2016

Nano Online

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Background: Magnetic resonance imaging is the ideal modality for non-invasive in vivo cell tracking allowing for longitudinal studies over time. Cells labeled with superparamagnetic iron oxide nanoparticles have been shown to induce sufficient contrast for in vivo magnetic resonance imaging enabling the in vivo analysis of the final location of the transplanted cells. For magnetic nanoparticles to be useful, a high internalization efficiency of the particles is required without compromising cell function, as well as validation of the magnetic nanoparticles behaviour inside the cells. Results: In this work, we report the development, optimization and validation of an efficient procedure to label human neural stem cells with commercial nanoparticles in the absence of transfection agents. Magnetic nanoparticles used here do not affect cell viability, cell morphology, cell differentiation or cell cycle dynamics. Moreover, human neural stem cells progeny labeled with magnetic nanoparticles are easily and non-invasively detected long time after transplantation in a rat model of Parkinson's disease (up to 5 months post-grafting) by magnetic resonance imaging. Conclusions: These findings support the use of commercial MNPs to track cells for short-and mid-term periods after transplantation for studies of brain cell replacement therapy. Nevertheless, long-term MR images should be interpreted with caution due to the possibility that some MNPs may be expelled from the transplanted cells and internalized by host microglial cells.

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Section: Resultsmentioning

confidence: 99%

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Ramos-Gómez¹,

Seiz²,

Martínez‐Serrano³

2016

Nano Online

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“…Various procedures for identifying neural progenitor cells using MRI have already been devised in rodents. 60,72 One group has identified a biomarker for neural progenitor cells and has used MR spectroscopy to analyze the biomarker in the hippocampus of live humans. 54 Further delineation of these methods could yield a promising technique for analyzing the impact of TBI in neurogenesis in children who survive.…”

Section: Discussionmentioning

confidence: 99%

Maturation-dependent response of neurogenesis after traumatic brain injury in children

Taylor

Smith²,

Harris

et al. 2013

PED

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Object Traumatic brain injury (TBI) is the leading cause of acquired disability in children, yet innate repair mechanisms are incompletely understood. Given data from animal studies documenting neurogenesis in response to trauma and other insults, the authors investigated whether similar responses could be found in children of different ages after TBI. Methods Immunohistochemistry was used to label doublecortin (DCX), a protein expressed by immature migrating neuroblasts (newborn neurons), in specimens from patients ranging in age from 3 weeks to 10 years who had died either after TBI or from other causes. Doublecortin-positive (DCX+) cells were examined in the subventricular zone (SVZ) and periventricular white matter (PWM) and were quantified within the granule cell layer (GCL) and subgranular zone (SGZ) of the dentate gyrus to determine if age and/or injury affect the number of DCX+ cells in these regions. Results The DCX+ cells decreased in the SVZ as patient age increased and were found in abundance around a focal subacute infarct in a 1-month-old non-TBI patient, but were scarce in all other patients regardless of age or history of trauma. The DCX+ cells in the PWM and dentate gyrus demonstrated a migratory morphology and did not co-localize with markers for astrocytes, microglia, or macrophages. In addition, there were significantly more DCX+ cells in the GCL and SGZ of the dentate gyrus in children younger than 1 year old than in older children. The density of immature migrating neuroblasts in infants (under 1 year of age) was significantly greater than in young children (2–6 years of age, p = 0.006) and older children (7–10 years of age, p = 0.007). Conclusions The main variable influencing the number of migrating neuroblasts observed in the SVZ, PWM, and hippocampus was patient age. Trauma had no discernible effect on the number of migrating neuroblasts in this cohort of patients in whom death typically occurred within hours to days after TBI.

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“…These particles lead to a marked decrease in the magnetic resonance imaging (MRI) parameter T2* and the possibility of visualizing their localization noninvasively on T2*-weighted MRI (7)(8)(9). Furthermore, the magnetic properties of SPIOs allow them to be manipulated mechanically by a magnetic field gradient.…”

Section: See Page 803mentioning

confidence: 99%

Magnetic Tagging Increases Delivery of Circulating Progenitors in Vascular Injury

Kyrtatos

Lehtolainen

Junemann-Ramirez³

et al. 2009

JACC: Cardiovascular Interventions

128

101

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In vivo magnetic resonance imaging of endogenous neuroblasts labelled with a ferumoxide–polycation complex

Cited by 44 publications

References 35 publications

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Maturation-dependent response of neurogenesis after traumatic brain injury in children

Magnetic Tagging Increases Delivery of Circulating Progenitors in Vascular Injury

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