Magnetic Tagging Increases Delivery of Circulating Progenitors in Vascular Injury

Kyrtatos, Panagiotis G.; Lehtolainen, Pauliina; Junemann-Ramirez, M; García‐Prieto, Ana; Price, Anthony N.; Martin, John F.; Gadian, David G.; Pankhurst, Quentin A.; Lythgoe, Mark F.

doi:10.1016/j.jcin.2009.05.014

Cited by 128 publications

(104 citation statements)

References 47 publications

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“…This could be achieved by direct infusion into the lumen of the target vessel, or by using novel approaches like magnetic tagging [25], ultrasound-generated acoustic radiation forces [26] or antibody capture to attract cells to the surface of the revascularized conduit (stent or graft). New catheter injection systems are also in development that will enable cells to be injected transluminally into the vascular adventitia and periadventitial space.…”

mentioning

confidence: 99%

Mechanistic Insights into Arterial Repair with Mesenchymal Stromal Cells

Psaltis

2011

Cardiovasc Drugs Ther

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mentioning

confidence: 99%

Mechanistic Insights into Arterial Repair with Mesenchymal Stromal Cells

Psaltis

2011

Cardiovasc Drugs Ther

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“…This compares favorably with endovascular seeding with EPCs loaded with supermagnetic iron oxide nanoparticles in arteries of similar caliber, where only up to sixfold enrichment over controls was obtained. 15,16 As shown in the composite image that lays out a length of treated aorta (Fig. 4D), the MSC adhesion was not homogeneous in spatial distribution around the endoluminal circumference, likely due to the tendency of the relatively soft Microsonic catheter to assume an eccentric position inside the vessel (Fig.…”

Section: Discussionmentioning

confidence: 98%

Vascular Endoluminal Delivery of Mesenchymal Stem Cells Using Acoustic Radiation Force

Toma

Fisher²,

Wang³

et al. 2011

Tissue Engineering Part A

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Restoration of functional endothelium is a requirement for preventing late stent thrombosis. We propose a novel method for targeted delivery of stem cells to a site of arterial injury using ultrasound-generated acoustic radiation force. Mesenchymal stem cells (MSCs) were surface-coated electrostatically with cationic gas-filled lipid microbubbles (mb-MSC). mb-MSC was characterized microscopically and by flow cytometry. The effect of ultrasound (5 MHz) on directing mb-MSC movement toward the vessel wall under physiologic flow conditions was tested in vitro in a vessel phantom. In vivo testing of acoustic radiation force-mediated delivery of mb-MSCs to ballooninjured aorta was performed in rabbits using intravascular ultrasound (1.7 MHz) during intra-aortic infusion of mb-MSCs. Application of ultrasound led to marginalization and adhesion of mb-MSCs to the vessel phantom wall, whereas no effect was observed on mb-MSCs in the absence of ultrasound. The effect was maximal when there were 7 -1 microbubbles/cell (n = 6). In rabbits (n = 6), adherent MSCs were observed in the ultrasound-treated aortic segment 20 min after the injection (334 -137 MSCs/cm 2 ), whereas minimal adhesion was observed in control segments not exposed to ultrasound (2 -1 MSCs/cm 2 , p < 0.05). At 24 h after mb-MSC injection and ultrasound treatment, the engrafted MSCs persisted and spread out on the luminal surface of the artery. The data demonstrate proof of principle that acoustic radiation force can target delivery of therapeutic cells to a specific endovascular treatment site. This approach may be used for endoluminal cellular paving and could provide a powerful tool for cell-based re-endothelialization of injured arterial segments.

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“…Permanent magnets offer scalable high field strength without the detrimental heating effects associated with solenoid-induced magnetic fields. We have previously magnetically tagged cells with SPION and targeted them to a localized site of arterial injury using a magnetic actuator consisting of a rectangular Halbach array positioned outside the body [23].…”

Section: Discussionmentioning

confidence: 99%

Rapid magnetic cell delivery for large tubular bioengineered constructs

Gonzalez‐Molina

Riegler

Southern

et al. 2012

J. R. Soc. Interface.

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Delivery of cells into tubular tissue constructs with large diameters poses significant spatial and temporal challenges. This study describes preliminary findings for a novel process for rapid and uniform seeding of cells onto the luminal surface of large tubular constructs. Fibroblasts, tagged with superparamagnetic iron oxide nanoparticles (SPION), were directed onto the luminal surface of tubular constructs by a magnetic field generated by a k4-type Halbach cylinder device. The spatial distribution of attached cells, as measured by the mean number of cells, was compared with a conventional, dynamic, rotational cell-delivery technique. Cell loading onto the constructs was measured by microscopy and magnetic resonance imaging. The different seeding techniques employed had a significant effect on the spatial distribution of the cells ( p , 0.0001). The number of attached cells at defined positions within the same construct was significantly different for the dynamic rotation technique ( p , 0.05). In contrast, no significant differences in the number of cells attached to the luminal surface were found between the defined positions on the construct loaded with the Halbach cylinder. The technique described overcomes limitations associated with existing cell-delivery techniques and is amenable to a variety of tubular organs where rapid loading and uniform distribution of cells for therapeutic applications are required.

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Magnetic Tagging Increases Delivery of Circulating Progenitors in Vascular Injury

Abstract: Using an externally applied magnetic device, we have been able to enhance EPC localization at a site of common carotid artery injury. This technology could be more widely adapted to localize cells in other organs and may provide a useful tool for the systemic injection of cell therapies.

Cited by 128 publications

References 47 publications

Mechanistic Insights into Arterial Repair with Mesenchymal Stromal Cells

Mechanistic Insights into Arterial Repair with Mesenchymal Stromal Cells

Vascular Endoluminal Delivery of Mesenchymal Stem Cells Using Acoustic Radiation Force

Rapid magnetic cell delivery for large tubular bioengineered constructs

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