In vivo investigation of the immersion-liquid-induced human skin clearing dynamics

Tuchin, Valery V.; Bashkatov, Alexey N.; Genina, Elina A.; Sinichkin, Yu. P.; Lakodina, Nina A.

doi:10.1134/1.1383834

Cited by 64 publications

(49 citation statements)

References 10 publications

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“…The obtained values agree well with the results of estimating the glucose diffusion coefficient (0.2 g/ml) in DM [23] [43], the glucose (40%-glucose solution was used) diffusion coefficient in human skin in vivo amounted to (2.56±0.13)×10 -6 cm 2 /s. Taking the difference of physical and optical properties of the studied tissues into account, we can conclude that the above values of the glucose diffusion coefficients agree well with the results, obtained in the present paper.…”

Section: Resultssupporting

confidence: 86%

Optical clearing of human dura mater by glucose solutions

Genina

Bashkatov

Tuchin

2017

JBPE

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Abstract. Significant light scattering by dura mater in the visible spectral range seriously restricts the application of noninvasive optical methods to the diagnostics and therapy of brain diseases. We present the results of the study of human dura mater optical clearing in vitro by the glucose solutions with the concentrations 1.5М and 3М in the spectral range 400-700 nm. The efficiency of the tissue optical clearing is assessed. Basing on the analysis of the kinetics of collimated transmittance of light by samples of dura mater, the relative glucose diffusion coefficients are calculated that amount to (1.10.1)10 -6 cm 2 /s and (2.00.2)10 -6 cm 2 /s, respectively.

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Section: Resultssupporting

confidence: 86%

Optical clearing of human dura mater by glucose solutions

Genina

Bashkatov

Tuchin

2017

JBPE

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“…Tuchin et al's result (8) does speak directly to dermal diffusivity, yet it is subject to uncertainties (e.g., osmotic and convective perturbations) related to the high glucose concentrations (20Y40% w/w) employed. Diffusivity and partitioning in viable epidermis have, to our knowledge, not been measured.…”

Section: Introductionmentioning

confidence: 96%

“…Based on the epidermal glucose level, these investigators suggested that glucose is not only restricted to the intercellular epidermal volume, but it also gained access to the available intracellular space by diffusion across the epidermal cell wall. Tuchin et al (8) used an optical method for the measurement of the diffusivity of glucose in human dermis in vivo and compared it to that in water at the same temperature. They concluded that glucose diffusion in the human dermis was hindered possibly by the structural components of the dermis.…”

Section: Introductionmentioning

confidence: 99%

Glucose Partition Coefficient and Diffusivity in the Lower Skin Layers

2006

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Purpose. This work aims to estimate the diffusivity and partitioning of glucose in the dermis and the viable epidermis of human skin. Methods. The partition coefficient of glucose between phosphate-buffered saline and dermis, tapestripped epidermis (TSE), stratum corneum (SC), and split-thickness skin, was measured in vitro using human cadaver skin. Glucose permeability across dermis and tape-stripped split-thickness skin (TSS) was measured using side-by-side diffusion cells. Glucose desorption from TSE and human epidermal membrane (HEM) was measured. All measurements were conducted at 32-C. Results. The partition coefficient for glucose [mean T SD (no. of samples)] was 0.65 T 0.09 (n = 25) for dermis, 0.81 T 0.06 (n = 10) for TSE, and 0.53 T 0.12 (n = 9) for SC. Glucose diffusivity in dermis was calculated to be 2.64 T 0.42 Â 10 j6 cm 2 /s (n = 14). Glucose diffusivities in the viable epidermis estimated from TSS permeation, TSE desorption, and HEM desorption were 0.075 T 0.050 Â 10 j6 cm 2 /s (n = 5), 0.037 T 0.018 Â 10 j6 cm 2 /s (n = 4), and 1.0 T 0.6 Â 10 j6 cm 2 /s (n = 4), respectively. Conclusion. The tissue/buffer partition coefficient of glucose in all skin layers was found to be less than unity, suggestive of excluded volumes in each layer. Glucose diffusivity in human dermis was found to be one third of its value in water, indicative of hindered diffusion related to the structural components of the tissue. A substantially lower value for glucose diffusivity in viable epidermis is suggested.

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“…In Vivo Skin: The change in reflectance spectrum has usually been used to assess in vivo skin clearing efficacy after treatment of OCAs [9,20]. One site of the prepared rat dorsal skin was selected as control group without light irradiation and the other site as experimental group with light irradiation.…”

Section: Experimental Instrumentsmentioning

confidence: 99%

Enhancement of skin optical clearing efficacy using photo‐irradiation

Liu¹,

Zhi²,

Tuchin³

et al. 2010

Lasers Surg Med

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Background and Objectives: Tissue optical clearing technique based on immersion of tissues into optical clearing agents (OCAs) can reduce the scattering and enhance the penetration of light in tissue. However, the barrier function of epidermis limits the penetration of OCAs, and hence is responsible for the poor optical clearing efficacy of skin by topical action. In this study, a variety of light irradiation was applied to increase permeability of agents in skin and improve the optical clearing efficacy. Study Design/Materials and Methods: Different light sources with different dose, i.e, CO 2 laser, Nd:YAG laser (532 and 1,064 nm) with different pulse modes and Intense Pulsed Light (IPL) (400-700 and 560-950 nm) were used to irradiate rat skin in vivo, and then glycerol was applied onto the irradiated zone. VIS-NIR spectrometer was utilized to monitor the changes of reflectance. In vitro skin samples were also irradiated by Q-switched Nd:YAG laser (1,064 nm) and then treated by glycerol for 10-60 minutes. Based on the measurement of the reflectance and transmittance of the samples, the optical properties of skin and penetration depth of light were calculated. Results: Results show that photo-irradiation with appropriate dose combining with the following glycerol treatment is able to reduce in vivo skin reflectance. Compared with the control group, the maximal changes in reflectance are ninefold at 575 nm and eightfold at 615 nm, respectively, which were caused by Q-switched 1,064-nm Nd:YAG laser irradiation and following glycerol treatment. The results for in vitro skin demonstrate that the joint action can significantly increase the optical penetration depth in samples. Conclusions: The combination of Q-switched Nd:YAG (1,064 nm) laser and glycerol could enhance optical skin clearing efficacy significantly. This study provides a noninvasive way to improve the optical clearing of skin, which will benefit the skin optical therapy.

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In vivo investigation of the immersion-liquid-induced human skin clearing dynamics

Cited by 64 publications

References 10 publications

Optical clearing of human dura mater by glucose solutions

Optical clearing of human dura mater by glucose solutions

Glucose Partition Coefficient and Diffusivity in the Lower Skin Layers

Enhancement of skin optical clearing efficacy using photo‐irradiation

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