In vivo imaging of spin-trapped nitric oxide in rats with septic shock: MRI spin trapping

Fujii, Hirotada; Wan, Xiao; Zhong, Jianhui; Berliner, Lawrence J.; Yoshikawa, Kohki

doi:10.1002/(sici)1522-2594(199908)42:2<235::aid-mrm4>3.0.co;2-y

Cited by 50 publications

(30 citation statements)

References 14 publications

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“…One way to detect NO using MRI was successfully demonstrated by Fujii et al . by using spin-trapping reagents, in a technique they called “MRI spin-trapping” 221. NO has been shown to form reasonably stable complexes with various Fe-based compounds.…”

Section: Chemical Exchange Saturation Transfer (Cest)mentioning

confidence: 99%

Alternatives to Gadolinium-Based Metal Chelates for Magnetic Resonance Imaging

et al. 2010

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Section: Chemical Exchange Saturation Transfer (Cest)mentioning

confidence: 99%

Alternatives to Gadolinium-Based Metal Chelates for Magnetic Resonance Imaging

et al. 2010

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“…After 20 minutes of stabilization, pressures were recorded for 30 minutes as described previously. 17 Then, the perfusate was changed to one containing an NO-trapping agent, N-methyl-D-glucamine dithiocarbamate (MGD)-Fe solution 18 (10 mmol/L MGD and 2 mmol/L Fe) for 1 minute and switched to the initial perfusate for 20 minutes. MGD was synthesized by the method of Shinobu et al 19 Next, the perfusate was changed to one containing an NO donor, S-nitroso-N-acetylpenicillamine (SNAP, 1 mmol/L, Sigma Chemical), for 1 minute and switched again to the initial perfusate for 15 minutes.…”

Section: Isolated Perfused Lung Experimentsmentioning

confidence: 99%

Angiotensin-Converting Enzyme Inhibitor Preserves p21 and Endothelial Nitric Oxide Synthase Expression in Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats

Kanno

Lee

et al. 2001

Circulation

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Background-Pulmonary arterial hypertension (PAH) is associated with structural changes in the pulmonary vasculature characterized by the proliferation of cellular components of the vessels. ACE inhibitor (ACEI) may have beneficial effects in treating PAH, but its precise mechanism of action in the remodeling process is unclear. p21 is a cyclin-dependent kinase inhibitor that may have a protective role in this process by inhibiting cellular proliferation. Endothelial nitric oxide synthase (eNOS) has also been shown to be protective by its vasodilatory effect. Therefore, we investigated whether expression of p21 and eNOS was modulated by ACEI treatment in a rat model. Methods and Results-Monocrotaline (MCT) was administered to 2 groups of Sprague-Dawley rats fed a high-cholesterol diet, ie, one group received MCT concomitantly with enalapril treatment (MCT ϩ /ACEI ϩ rats), and the other group did not receive enalapril (MCT ϩ /ACEI Ϫ rats). After 5 weeks, MRI showed right ventricular hypertrophy in MCT ϩ /ACEI

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“…This complex appeared as more stable in vivo and a more effective MRI contrast agent than other stable N-containing free radicals [121]. A similar approach was recently attempted by proton-electron-double-resonance imaging (PEDRI), making use of the nitroprusside-derived radical, the DNIC of NO with the iron-serum albumin complex and the MNIC with MGD [122].…”

Section: Three-dimensional Epr Imaging and Other Recent Methodologicamentioning

confidence: 95%

“…The paramagnetic ternary NO-Fe 2+ -(MGD) 2 complex has been tested to use as a magnetic resonance imaging (MRI) contrast agent [121]. This complex appeared as more stable in vivo and a more effective MRI contrast agent than other stable N-containing free radicals [121].…”

Section: Three-dimensional Epr Imaging and Other Recent Methodologicamentioning

confidence: 99%

Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems

Henry¹,

Guissani²

2000

Analusis

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In this overview of NO spin-trapping methods, we have pointed out their main advantages over frozen solution EPR spectroscopy detection of nitrosylated metalloproteins: improved sensitivity, potential specificity and time-resolution. A few simple ideas concerning EPR methodology have been recalled. Examples of possible artefacts, sometimes unexpected, which can easily be encountered in any analytical methods have been described. New methodological developments have also been mentioned.

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In vivo imaging of spin-trapped nitric oxide in rats with septic shock: MRI spin trapping

Cited by 50 publications

References 14 publications

Alternatives to Gadolinium-Based Metal Chelates for Magnetic Resonance Imaging

Alternatives to Gadolinium-Based Metal Chelates for Magnetic Resonance Imaging

Angiotensin-Converting Enzyme Inhibitor Preserves p21 and Endothelial Nitric Oxide Synthase Expression in Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats

Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems

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