In vivo imaging of intrinsic optical signals in chicken retina with functional optical coherence tomography

Moayed, Alireza Akhlagh; Hariri, Sanaz; Choh, Vivian; Bizheva, Kostadinka

doi:10.1364/ol.36.004575

Cited by 26 publications

(28 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…11 Micrometer level (<3 μm) resolution has been achieved for in vivo IOS imaging of frog retinas using custom-designed confocal 12 and OCT 13 systems. Stimulus-evoked IOSs have been observed in multiple animal models [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] and human subjects. 29 Recent OCT studies revealed rapid IOS changes at photoreceptor outer segments.…”

Section: Introductionmentioning

confidence: 99%

In vivooptical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

Wang

Yao

2016

J. Biomed. Opt

View full text Add to dashboard Cite

Abstract. Intrinsic optical signal (IOS) imaging promises a noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, it is essential to understand anatomic and physiological sources of retinal IOSs and to establish the relationship between IOS distortions and eye diseases. The purpose of this study was designed to demonstrate the feasibility of in vivo IOS imaging of mouse models. A high spatiotemporal resolution spectral domain optical coherence tomography (SD-OCT) was employed for depth-resolved retinal imaging. A custom-designed animal holder equipped with ear bar and bite bar was used to minimize eye movements. Dynamic OCT imaging revealed rapid IOS from the photoreceptor's outer segment immediately after the stimulation delivery, and slow IOS changes were observed from inner retinal layers.Comparative photoreceptor IOS and electroretinography recordings suggested that the fast photoreceptor IOS may be attributed to the early stage of phototransduction before the hyperpolarization of retinal photoreceptor.

show abstract

Section: Introductionmentioning

confidence: 99%

In vivooptical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

Wang

Yao

2016

J. Biomed. Opt

View full text Add to dashboard Cite

show abstract

“…The fOCT B-scans were aligned in 3D stacks 18 and processed with ImageJ software to correct for motion artefacts arising from involuntary eye motion and heart rate. Successive B-scans were cross-correlated to compensate for linear shifts in the x and z directions (along the imaged strip and the retinal depth, respectively) and for rotational misalignment.…”

Section: Resultsmentioning

confidence: 99%

“…18 Briefly, the fOCT system is based on spectral domain OCT technology and is designed to operate in the 1,060 nm wavelength range. This spectral region was chosen to ensure no visual stimulation of the retinal photoreceptors by the imaging beam.…”

Section: Methodsmentioning

confidence: 99%

“…The fOCT system's data acquisition was synchronized with that of a commercial ERG system (Diagnosys LLC, Lowell, MA, USA). A custom-designed visual stimulator integrated with the optical imaging probe and described in detail in our earlier publication 18 was used to project a ∼2 mm diameter spot on the retinal surface with preselected color, duration, and intensity of the visual stimulus.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Correlation of visually evoked intrinsic optical signals and electroretinograms recorded from chicken retina with a combined functional optical coherence tomography and electroretinography system

et al. 2012

View full text Add to dashboard Cite

Abstract. Visually evoked fast intrinsic optical signals (IOSs) were recorded for the first time in vivo from all layers of healthy chicken retina by using a combined functional optical coherence tomography (fOCT) and electroretinography (ERG) system. The fast IOSs were observed to develop within ∼5 ms from the on-set of the visual stimulus, whereas slow IOSs were measured up to 1 s later. The visually evoked IOSs and ERG traces were recorded simultaneously, and a clear correlation was observed between them. The ability to measure visually evoked fast IOSs non-invasively and in vivo from individual retinal layers could significantly improve the understanding of the complex communication between different retinal cell types in healthy and diseased retinas.

show abstract

“…OCT intensity measurements have been used to detect visually evoked cortical functions in cat brain; [17][18][19] electrically evoked (forepaw stimulation) somatosensory changes in rat brain; 20,21 and light activated changes in frog, 22 rabbit, 23 rat, 24 chicken, 25 and human 26 retinas. Also, the intensity signals due to electrical stimulation of the abdominal ganglion of sea slug 27,28 and squid giant axon 29 have been reported.…”

Section: Introductionmentioning

confidence: 99%

Optical coherence tomography for cross-sectional imaging of neural activity

et al. 2015

View full text Add to dashboard Cite

Abstract. We report a functional optical coherence tomography cross-sectional scanner to detect neural activity using unmyelinated nerves dissected from squid. The nerves, unstained or stained with a voltage-sensitive dye, were imaged in a nerve chamber. Transient phase changes from backscattered light were detected during action potential propagation. The results show that the scanner can provide high spatiotemporal resolution crosssectional images of neural activity (15 μs∕A-line; 0.25 ms∕B-scan; ∼8.5 × 5.5 μm 2 in xz). The advantage of this method compared to monitoring a single depth profile z is a dramatic increase in the number of available sites that can be measured in two spatial dimensions xz with lateral scanning; therefore, the study demonstrates that two-dimensional monitoring of small-scale functional activity would also be feasible.

show abstract

In vivo imaging of intrinsic optical signals in chicken retina with functional optical coherence tomography

Cited by 26 publications

References 12 publications

In vivooptical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

In vivooptical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

Correlation of visually evoked intrinsic optical signals and electroretinograms recorded from chicken retina with a combined functional optical coherence tomography and electroretinography system

Optical coherence tomography for cross-sectional imaging of neural activity

Contact Info

Product

Resources

About