In vivo imaging of dynamic biological specimen by real-time single-shot full-field optical coherence tomography

Full-Field Optical Coherence Microscopy

“…For these kinds of applications, a shorter acquisition time is definitely required. High speed systems have been proposed [45][46][47][48][49], making in vivo imaging possible. However, the detection sensitivity of these systems is quite low.…”

“…Artifacts resulting from transverse motion can even be completely removed if the frames are acquired simultaneously and not sequentially in time. For that purpose, instantaneous phase-shifting interferometry was successfully applied in FF-OCM, at the price of an increase in system complexity, calibration and cost [45,46,48,50]. Fig.…”

1550 nm Superluminescent Diode and Anti-Stokes Effect CCD camera based Optical Coherence Tomography for Full-Field Optical Metrology

“…A FF-OCT system, using pulse illumination, suppressing motion artifacts originating from sample lateral movements, was designed for in vivo imaging [95]. A single-shot FF-OCT is a technique able to record all phase-shifted interferograms simultaneously to increase speed of operation and a potentially suitable for in-vivo imaging [76,88]. There are successful approaches of full-field type of image acquisition in the frequency domain, either by using frequency tuned laser [96,97] or an SLD with an acusto-optic tunable filter [98,99].…”

Snapshot 3D optical coherence tomography system using image mapping spectrometry

“…Full-Field OCT as exemplified by AC Boccara et al can provide real time in vivo imaging without lateral scanning, albeit with acquisition of multiple phase-shifted images, rather than single-shot [14]. Subhash et al demonstrated a version of FF-OCT where the requisite phase-stepped images are all captured in a single camera snap-shot by distributing each image to a separate region of the image sensor [15,16]. This method could, therefore, provide snapshot en face (XY) FF-OCT imaging at a single axial (Z) location; however, generation of a 3D volume required recording of multiple camera acquisitions.…”