In Vivo Imaging and Morphometry of the Human Pre-Descemet's Layer and Endothelium With Ultrahigh-Resolution Optical Coherence Tomography

Bizheva, Kostadinka; Haines, Lacey; Mason, Erik; MacLellan, Benjamin; Tan, Bingyao; Hileeto, Denise; Sorbara, Luigina

doi:10.1167/iovs.15-18936

Cited by 41 publications

(29 citation statements)

References 22 publications

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“…In contrast to commercial systems, we were also able to distinguish endothelium from Descemet's membrane. Recently, an UHR-OCT prototype based on a supercontinuum laser as light source has been presented allowing in vivo imaging and thickness measurements of the human Pre-Descemet's layer and endothelium [56].…”

Section: Discussionmentioning

confidence: 99%

Ultrahigh-resolution OCT imaging of the human cornea

Werkmeister¹,

Sapeta²,

Schmidl³

et al. 2017

Biomed. Opt. Express

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We present imaging of corneal pathologies using optical coherence tomography (OCT) with high resolution. To this end, an ultrahigh-resolution spectral domain OCT (UHR-OCT) system based on a broad bandwidth Ti:sapphire laser is employed. With a central wavelength of 800 nm, the imaging device allows to acquire OCT data at the central, paracentral and peripheral cornea as well as the limbal region with 1.2 µm x 20 µm (axial x lateral) resolution at a rate of 140 000 A-scans/s. Structures of the anterior segment of the eye, not accessible with commercial OCT systems, are visualized. These include corneal nerves, limbal palisades of Vogt as well as several corneal pathologies. Cases such as keratoconus and Fuchs's endothelial dystrophy as well as infectious changes caused by diseases like Acanthamoeba keratitis and scarring after herpetic keratitis are presented. We also demonstrate the applicability of our system to visualize epithelial erosion and intracorneal foreign body after corneal trauma as well as chemical burns. Finally, results after Descemet's membrane endothelial keratoplasty (DMEK) are imaged. These clinical cases show the potential of UHR-OCT to help in clinical decision-making and follow-up. Our results and experience indicate that UHR-OCT of the cornea is a promising technique for the use in clinical practice, but can also help to gain novel insight in the physiology and pathophysiology of the human cornea.

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Section: Discussionmentioning

confidence: 99%

Ultrahigh-resolution OCT imaging of the human cornea

Werkmeister¹,

Sapeta²,

Schmidl³

et al. 2017

Biomed. Opt. Express

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“…However, the PDL has been demonstrated in vivo in humans by ultrahigh‐resolution OCT (Bizheva et al. ). This mode of examination may allow imaging of the fenestrations.…”

Section: Discussionmentioning

confidence: 99%

“…); and its recent demonstration in vivo by ultrahigh‐resolution OCT (Bizheva et al. ), all point to its unique nature.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of big bubble formation in deep anterior lamellar keratoplasty by the big bubble technique: in vitro studies

Dua

Faraj

Kenawy

et al. 2017

Acta Ophthalmologica

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“…OCT is a widely used, nondestructive and noninvasive 3D interferometry microscopy technique. With a resolution of only a few micrometers (up to less than 1 μm with ultra high resolution systems) and a depth of penetration of several mm, OCT has become an essential tool in ophthalmology and neuro‐ophthalmology . OCT is based on near‐infrared spectroscopy and allows the observation of cornea and retina images of patients without causing damage …”

Section: Gold Nanoparticles For Diagnosis In Ophthalmologymentioning

confidence: 99%

Gold nanoparticles in ophthalmology

Masse

Ouellette

Lamoureux

et al. 2018

Medicinal Research Reviews

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Many research projects are underway to improve the diagnosis and therapy in ophthalmology. Indeed, visual acuity deficits affect 285 million people worldwide and different strategies are being developed to strengthen patient care. One of these strategies is the use of gold nanoparticles (GNP) for their multiple properties and their ability to be used as both diagnosis and therapy tools. This review exhaustively details research developing GNPs for use in ophthalmology. The toxicity of GNPs and their distribution in the eye are described through in vitro and in vivo studies. All publications addressing the pharmacokinetics of GNPs administered in the eye are extensively reviewed. In addition, their use as biosensors or for imaging with optical coherence tomography is illustrated. The future of GNPs for ophthalmic therapy is also discussed. GNPs can be used to deliver genes or drugs through different administration routes. Their antiangiogenic and anti-inflammatory properties are of great interest for different ocular pathologies. Finally, GNPs can be used to improve stereotactic radiosurgery, brachytherapy, and photothermal therapy because of their many properties.

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In Vivo Imaging and Morphometry of the Human Pre-Descemet's Layer and Endothelium With Ultrahigh-Resolution Optical Coherence Tomography

Cited by 41 publications

References 22 publications

Ultrahigh-resolution OCT imaging of the human cornea

Ultrahigh-resolution OCT imaging of the human cornea

Dynamics of big bubble formation in deep anterior lamellar keratoplasty by the big bubble technique: in vitro studies

Gold nanoparticles in ophthalmology

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