In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

Ritsma, Laila; Vrisekoop, Nienke; Rheenen, Jacco van

doi:10.1038/ncomms3366

Cited by 34 publications

(23 citation statements)

References 20 publications

(25 reference statements)

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“…4 , or the tissue can be stabilized with the PEEP protocol described by Kimura et al 20 . In these cases, fiducial marks for microcartography may be added directly to the tissue using the photo-tattooing method described by Ritsma et al 21 . In all cases, it is crucial to include appropriate controls so as to isolate the impact of the surgical protocol from the biology being studied.…”

Section: Discussionmentioning

confidence: 99%

A permanent window for the murine lung enables high-resolution imaging of cancer metastasis

et al. 2017

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Stable, high-resolution intravital imaging of the lung has become possible through the utilization of vacuum-stabilized imaging windows. However, this technique is extremely invasive and limited to only hours in duration. Here we describe a minimally invasive, permanently implantable window for high-resolution intravital imaging of the murine lung that allows the mouse to survive surgery, recover from anesthesia, and breathe independently. Compared to vacuum-stabilized windows, this window produces the same high-quality images without vacuum-induced artifacts; it is also less invasive, which allows imaging of the same lung tissue over a period of weeks. We further adapt the technique of microcartography for reliable relocalization of the same cells longitudinally. using commonly employed experimental, as well as more clinically relevant, spontaneous metastasis models, we visualize all stages of metastatic seeding, including: tumor cell arrival; extravasation; growth and progression to micrometastases; as well as tumor microenvironment of metastasis function, the hallmark of hematogenous dissemination of tumor cells.

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Section: Discussionmentioning

confidence: 99%

A permanent window for the murine lung enables high-resolution imaging of cancer metastasis

et al. 2017

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“…For instance, multiphoton microscopy, which achieves relatively deep penetration, may be used for intravital imaging of animal models and correlated to a later acquired EM image 96,97 . Improvements of intravital FM-including fast, high-throughput 3D fluorescence imaging approaches such as light-sheet microscopy 86 -may be applied, and relocating ROIs using laser-based marks ('tattooing') 96 will be important for intravital CLEM. With EM, structural information within whole living organisms can be obtained, as has been applied to study zebrafish [98][99][100] , mouse neuronal circuits 101 and tumor cell invasion in mice 97 .…”

Section: Future Outlook Probes and Live-cell Electron Microscopymentioning

confidence: 99%

Correlated light and electron microscopy: ultrastructure lights up!

2015

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Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek observed living microorganisms and cells in 1674 using his light microscope. A spectrum of dyes and probes now enable the localization of molecules of interest within living cells by fluorescence microscopy. With electron microscopy (EM), cellular ultrastructure has been revealed. Bridging these two modalities, correlated light microscopy and EM (CLEM) opens new avenues. Studies of protein dynamics with fluorescent proteins (FPs), which leave the investigator 'in the dark' concerning cellular context, can be followed by EM examination. Rare events can be preselected at the light microscopy level before EM analysis. Ongoing development-including of dedicated probes, integrated microscopes, large-scale and three-dimensional EM and super-resolution fluorescence microscopy-now paves the way for broad CLEM implementation in biology.

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“…Towards that direction, one recent study has integrated IVM measurements with immunohistochemistry of the same tissue for greater biological context [281], and new methods for high-content immunofluorescence [282,283], multiplexed mass spectrometry imaging [284], and in situ RNA sequencing [285] will be powerful complementary tools. Computational modeling of complex and stochastic biological networks has proven useful in understanding cell behavior and drug response, for instance in the context of biomechanical influences on cell signaling and behavior [286]; complex intracellular networks of kinase [287–289] and/or transcriptional activity [290]; metabolic networks [291,292]; reaction/diffusion processes of receptor signaling and antibody therapies [272]; dynamic feedback and PD response [293,294]; along with multi-scale and agent-based models of multicellular behavior [295,296].…”

Section: The Nuts and Bolts Of Ivm Nanoparticle Imagingmentioning

confidence: 99%

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Miller

Weissleder

2017

Advanced Drug Delivery Reviews

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Therapeutic nanoparticles (NPs) can deliver cytotoxic chemotherapeutics and other drugs more safely and efficiently to patients; furthermore, selective delivery to target tissues can theoretically be accomplished actively through coating NPs with molecular ligands, and passively through exploiting physiological “enhanced permeability and retention” features. However, clinical trial results have been mixed in showing improved efficacy with drug nano-encapsulation, largely due to heterogeneous NP accumulation at target sites across patients. Thus, a clear need exists to better understand why many NP strategies fail in vivo and not result in significantly improved tumor uptake or therapeutic response. Multicolor in vivo confocal fluorescence imaging (intravital microscopy; IVM) enables integrated pharmacokinetic and pharmacodynamic (PK/PD) measurement at the single-cell level, and has helped answer key questions regarding the biological mechanisms of in vivo NP behavior. This review summarizes progress to date and also describes useful technical strategies for successful IVM experimentation.

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In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

Cited by 34 publications

References 20 publications

A permanent window for the murine lung enables high-resolution imaging of cancer metastasis

A permanent window for the murine lung enables high-resolution imaging of cancer metastasis

Correlated light and electron microscopy: ultrastructure lights up!

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

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