In vivo glucose metabolism in individual tissues of the rat. Interaction between epinephrine and insulin.

James, David E.; Burleigh, K. M.; Kraegen, Edward W.

doi:10.1016/s0021-9258(19)84571-4

Cited by 123 publications

(4 citation statements)

References 34 publications

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“…in agreement with other studies (5,12,14). Epinephrine was in fact such an effective inhibitor of insulinstimulated glucose phosphorylation that glucose phosphorylation was reduced to a level similar to that under basal conditions.…”

Section: E453 Epinephrine and Glucose Metabolism During Contractionsupporting

confidence: 93%

“…Epinephrine is believed to be the most important inhibitor. During insulin stimulation, it is well documented that epinephrine decreases glucose clearance in vivo (32) and, in agreement with this, epinephrine inhibits insulin-stimulated glucose uptake in skeletal muscles (5,12,14).…”

mentioning

confidence: 54%

“…In this study, we have investigated basic mechanisms for epinephrine-induced inhibition of glucose metabolism in contracting skeletal muscle. As epinephrine may reduce insulin-stimulated glucose uptake to a larger extent in oxidative muscles than in glycolytic muscles (8,12), the study was performed with a fasttwitch glycolytic muscle (epitrochlearis) and a slowtwitch oxidative muscle (soleus). We report here that epinephrine reduces glucose phosphorylation much less during contractile activity than during insulin stimulation despite similar glucose 6-phosphate concentrations.…”

mentioning

confidence: 99%

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Effects of epinephrine on glucose metabolism in contracting rat skeletal muscles

Aslesen

Jensen

1998

American Journal of Physiology-Endocrinology and Metabolism

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The effects of epinephrine on glucose metabolism during contractile activity and insulin stimulation were investigated in fast-twitch (epitrochlearis) and slow-twitch (soleus) muscles from Wistar rats. All muscles were mounted on contraction apparatuses, and some muscles were stimulated electrically for 30 min in vitro. Glucose uptake and glucose phosphorylation were measured with 2-[1,2-3H(N)]deoxy-d-glucose and glucose transport with 3- O-[ methyl-3H]methyl-d-glucose.d-[1-14C]mannitol was used to correct for extracellular space. In epitrochlearis, both contraction and insulin increased glucose transport by threefold, and combined they showed an additive effect. Epinephrine (10−6 M) did not influence glucose transport across the membrane during contractile activity or insulin stimulation. In the absence of epinephrine, similar glucose phosphorylation was obtained during contraction and during insulin stimulation in epitrochlearis (∼12 mmol ⋅ kg dry wt−1 ⋅ 30 min−1). In the presence of epinephrine, 9.5 ± 0.6 mmol ⋅ kg dry wt−1 ⋅ 30 min−1 glucose was phosphorylated during contraction, whereas only 2.0 ± 0.3 mmol ⋅ kg dry wt−1 ⋅ 30 min−1 was phosphorylated during insulin stimulation ( P < 0.01), despite a similar glucose 6-phosphate concentration. Comparable results were obtained in soleus. In conclusion, our data suggest that epinephrine inhibits glucose phosphorylation much less during contraction than during insulin stimulation.

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Section: E453 Epinephrine and Glucose Metabolism During Contractionsupporting

confidence: 93%

mentioning

confidence: 54%

mentioning

confidence: 99%

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Effects of epinephrine on glucose metabolism in contracting rat skeletal muscles

Aslesen

Jensen

1998

American Journal of Physiology-Endocrinology and Metabolism

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“…Insulin is furthermore important for the substrate supply of brown adipose tissue . The rate of glucose transport in brown adipocytes is highly increased by insulin (22) .…”

Section: Brown Adipocyte Thermogenesis Is Regulated By Several Hormonal Factorsmentioning

confidence: 99%

Development of Phodopus sungorus brown preadipocytes in primary cell culture: effect of an atypical beta-adrenergic agonist, insulin, and triiodothyronine on differentiation, mitochondrial development, and expression of the uncoupling protein UCP.

Schimrigk

Cassard-Doulcier

Ricquier

1991

The Journal of Cell Biology

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Abstract. A new cellular model for the study of brown adipocyte development and differentiation in vitro is presented . Preadipocytes isolated from brown adipose tissue (BAT) of the djungarian dwarf hamster Phodopus sungorus are able to proliferate and differentiate in vitro into true brown adipocytes able to express the BAT marker protein the uncoupling protein (UCP) . Whereas basal UCP expression is very low, its mRNA levels as well as the UCP detected by immunoblotting are highly increased by 0-adrenergic stimulation. The novel, atypical 0-adrenergic compound D7114 (ICI Pharmaceuticals, Macclesfield, Cheshire, T wo types of adipose tissue with quite different functions are known to exist in mammals : firstly the white adipose tissue whose main function is energy storage, secondly the so-called brown adipose tissue (BAT)', which functions as a heat dissipating organ in small and newborn mammals . This function is due to the uncoupling protein (UCP), uniquely expressed in BAT, which is located in the inner mitochondrial membrane (for review see 24) . UCP functions as a proton translocator and can thus short circuit the coupling between the respiratory chain and ATP production, resulting in elevated respiration rates and dissipation of the energy as heat (28). Contrary to white fat, the BAT is highly innervated and vascularized and brown adipocytes have very high respiratory capacities, i .e., a high content of mitochondria. Brown fat is considered to play an important function in energy balance, by dissipating excess energy intake as heat in certain animal models (38). It is also known that many types of obesity in animals are associated with a defective BAT function (17) . Despite their quite contrary physiological function, it is not yet clear if brown and white adipocytes should be considered as two distinctly different cell types or iftheyjust represent two different differentiation or maturation states of the same cell type. According to our present knowledge, every adipocyte that expresses UCP is England) was found to increase the number of adipocytes as well as UCP mRNA and UCP content of mitochondria, indicating the involvement of an atypical or /33 receptor. Insulin was found to play an important role in brown adipocyte differentiation and mitochondria) development, whereas T3 seemed to be implicated more directly in UCP expression . In a defined, serum-free medium a synergistic stimulatory action of insulin and T3 on UCP expression was found, which seems to involve a pathway different from that of 0-adrenergic UCP stimulation . considered a brown adipocyte, whereas an adipocyte not expressing UCP is not automatically a white adipocyte. In small mammals, like rodents, BAT preserves throughout the animal life its thermogenic ability, i .e., the ability to express UCP. But in larger mammals, like bovine and ovine species, itwas shown that fat depots ofa true brown nature are present at birth, which very rapidly lose their "brown" nature and become typical white depots which will never express UCP anymore...

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Glucose Utilization

Richter

1996

Comprehensive Physiology

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The sections in this article are: Molecular Basis for Glucose Transport Family of Glucose Transporters Translocation of Glucose Transporters Intrinsic Activity Fiber‐Type Specific Expression of GLUT 4 Role of Glut4 in Contraction‐Induced Skeletal Muscle Glucose Transport Signaling Mechanisms Involved in Contraction‐Induced Increase in Glucose Transport Regulation of Glucose Utilization in Vivo Glucose Supply Membrane Glucose Transport Capacity Glucose Metabolism Regulation of Glucose Utilization at Rest Regulation of Glucose Utilization During Exercise Effect of Exercise Intensity Effect of Exercise Duration Effect of Exercise Type Alternative Substrates Glucose Utilization During Exercise in Adverse Environment Humoral Regulation of Glucose Utilization During Exercise Effect of Physical Training Status Exercise in States of Altered Glucose Utilization Regulation of Glucose Utilization in the Postexercise State Membrane Glucose Transport Capacity Induced by Contraction Insulin Sensitivity

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In vivo glucose metabolism in individual tissues of the rat. Interaction between epinephrine and insulin.

Cited by 123 publications

References 34 publications

Effects of epinephrine on glucose metabolism in contracting rat skeletal muscles

Effects of epinephrine on glucose metabolism in contracting rat skeletal muscles

Development of Phodopus sungorus brown preadipocytes in primary cell culture: effect of an atypical beta-adrenergic agonist, insulin, and triiodothyronine on differentiation, mitochondrial development, and expression of the uncoupling protein UCP.

Glucose Utilization

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