In vivo functional characterisation of pheromone binding protein-1 in the silkmoth, Bombyx mori

Shiota, Yusuke; Sakurai, Takeshi; Daimon, Takaaki; Mitsuno, Hidefumi; Fujii, Takeshi; Matsuyama, Shigeru; Sezutsu, Hideki; Ishikawa, Yukio; Kanzaki, Ryohei

doi:10.1038/s41598-018-31978-2

Cited by 36 publications

(33 citation statements)

References 41 publications

(46 reference statements)

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“…To test whether expression of olfactory system genes in the antennae were affected by loss of sex determination pathway genes, we quantified expression of genes that encode factors necessary for mate recognition by males, notably, pheromone binding proteins BmPBP1, BmPBP2, and BmPBP3 and odorant receptors BmOR1, BmOR2, and BmOR3 [45]. BmPBP1 binds to bombykol, and its dysfunction causes the failure of male courtship behavior [41]. Although BmPBP1 was expressed at wild-type levels in males mutant in Bmfru , it was significantly decreased in the males mutant in BmMasc , BmPSI , and Bmdsx .…”

Section: Resultsmentioning

confidence: 99%

Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade

Liu

Yang

et al. 2020

Preprint

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25Insect courtship and mating depend on integration of olfactory, visual, and tactile cues. 26 Compared to other insects, Bombyx mori, the domesticated silkworm, has relatively 27 simple sexual behaviors as it cannot fly. Here by using CRISPR/Cas and 28 electrophysiological techniques we found that courtship and mating behaviors are 29 regulated in male silk moths by mutating genes in the sex determination cascade 30 belonging to two conserved pathways. Loss of Bmdsx gene expression significantly 31 reduced the peripheral perception of the major pheromone component bombykol by 32 reducing expression of the product of the BmOR1 gene which completely blocked 33 courtship in adult males. Interestingly, we found that mating behavior was regulated 34 independently by another sexual differentiation gene, Bmfru. Loss of Bmfru completely 35 blocked mating, but males displayed normal courtship behavior. Lack of Bmfru 36 expression significantly reduced the perception of the minor pheromone component 37 bombykal due to the down regulation of BmOR3 expression; further, functional analysis 38 revealed that loss of the product of BmOR3 played a key role in terminating male mating 39 behavior. Our results suggest that Bmdsx and Bmfru are at the base of the two primary 40 pathways that regulate olfactory-based sexual behavior. 41 42 genome editing 43 44 45 46 3 47 Author Summary 48 The fundamental insect sexual behaviors, courtship and mating, result from successful 49 integration of olfactory, vision, tactile and other complex innate behaviors. In the widely 50 used insect model, Drosophila melanogaster, the sex determination cascade genes 51 fruitless and doublesex are involved in the regulation of courtship and mating behaviors; 52 however, little is known about the function of these sexual differentiation genes in 53 regulating sex behaviors of Lepidoptera. Here we combine genetics and 54 electrophysiology to investigate regulation pathway of sexual behaviors in the model 55 lepidopteran insect, the domesticated silk moth, Bombyx mori. Our results support the 56 presence of two genetic pathways in B. mori, named Bmdsx-BmOR1-bombykol and 57 Bmfru-BmOR3-bombykal, which control distinct aspects of male sexual behavior that are 58 modulated by olfaction. This is the first comprehensive report about the role of sex 59 differentiation genes in the male sexual behavior in the silk moth.60 61 62 63 64 65 66 67 68 4 69 Introduction 70 Sex determination pathways control the sexually dimorphic traits of males and females, 71 including sexual differentiation and sexual behavior [1]. The genetic cascades of primary 72 signalling that underlie sex determination in insects have high diversity among species. In 73 the model insect Drosophila melanogaster, sex determination is controlled hierarchically 74 by X:A, Sex-lethal (Sxl), transformer or transformer 2 (tra/tra2), doublesex (dsx), and 75 fruitless (fru) [2, 3]. An X:A ratio of 1 promotes transcription of Sxl and results in 76 feminization, while an X:A ratio of 0.5 results in Sxl ...

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Section: Resultsmentioning

confidence: 99%

Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade

Liu

Yang

et al. 2020

Preprint

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“…Many research reported at least one of the PBPs can well bind with sex pheromones [29][30][31] . In our analysis, PBP2 showed significantly male bias expression, and PBP1, PBP3…”

Section: Discussionmentioning

confidence: 99%

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Jing

Zhang

Bai

et al. 2019

Preprint

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Background Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results Totally, 26 odorant-binding proteins (OBP) genes, 19 chemosensory proteins (CSP) genes, 55 odorant receptors (OR) genes and 20 ionotropic receptors (IR) genes were identified from the C. pinicolalis antennae transcriptome and most of them were olfactory genes, amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we strongly recommended the GAPDH or RP49 to verify gene expression for larvae development and RP49 or RPL13 for adult tissues. Conclusions Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be a supportive for pest management studies in future.

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“…pathway genes, we quantified expression of genes that encode factors necessary for mate recognition by males, notably, pheromone binding proteins BmPBP1, BmPBP2, and BmPBP3 and odorant receptors BmOR1, BmOR2, and BmOR3 [45]. BmPBP1 binds to bombykol, and its dysfunction causes the failure of male courtship behavior [41]. Although BmPBP1 was expressed at wild-type levels in male mutants in Bmfru, it was significantly decreased in the males mutant in BmMasc, BmPSI, and Bmdsx.…”

Section: Expression Of Male-biased Olfactory System Genes Is Altered mentioning

confidence: 99%

“…The pheromone binding protein BmPBP1, which has a male-biased expression, is required for the selectivity of BmOR1 for bombykol [39]. Loss of function mutations in BmPBP1 or BmOR1 cause the disappearance of male sexual behavior [40,41]. Previous studies showed that the sex determination gene Bmdsx controls the expression of BmPBP1 and of BmOR1 [42,14].…”

Section: Introductionmentioning

confidence: 99%

Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade

Liu

Yang

et al. 2020

PLoS Genet

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Insect courtship and mating depend on integration of olfactory, visual, and tactile cues. Compared to other insects, Bombyx mori, the domesticated silkworm, has relatively simple sexual behaviors as it cannot fly. Here by using CRISPR/Cas9 and electrophysiological techniques we found that courtship and mating behaviors are regulated in male silk moths by mutating genes in the sex determination cascade belonging to two conserved pathways. Loss of Bmdsx gene expression significantly reduced the peripheral perception of the major pheromone component bombykol by reducing expression of the product of the BmOR1 gene which completely blocked courtship in adult males. Interestingly, we found that mating behavior was regulated independently by another sexual differentiation gene, Bmfru. Loss of Bmfru completely blocked mating, but males displayed normal courtship behavior. Lack of Bmfru expression significantly reduced the perception of the minor pheromone component bombykal due to the down regulation of BmOR3 expression; further, functional analysis revealed that loss of the product of BmOR3 played a key role in terminating male mating behavior. Our results suggest that Bmdsx and Bmfru are at the base of the two primary pathways that regulate olfactory-based sexual behavior.

show abstract

In vivo functional characterisation of pheromone binding protein-1 in the silkmoth, Bombyx mori

Cited by 36 publications

References 41 publications

Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade

Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade

Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade

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