In vivo exposure of carp to graded concentrations of bisphenol A

Adverse effects of bisphenol A on the testicular parenchyma of zebrafish revealed using histomorphological methods

“…These observations are in contrast to those made in the study of Mandich et al (2007), who, after exposure of carp to the same concentration of BPA, described a reduction in the sperm ducts and the lobule diameter. Similar observations were made in a study on fathead minnows (Pimephales promelas), in which a diminution was observed in the proportion of the different types of cells (an increase in the proportion of spermatocytes) after exposure to 1 µg/l of BPA (Sohoni et al 2001).…”

“…In agreement with previously reported data, no mortalities occurred during the BPA exposure period (Hatef et al 2012;Villaneuve et al 2012), nor were there any differences with regard to the weight and length of the animals after the study period. These data are in accordance with those previously reported by Mandich et al (2007) and with our own earlier study (Molina et al 2013). However, the macroscopic aspect of the gonads remained apparently normal in our study, whereas other authors, after a period of exposure to 17-β-oestradiol, noted an attenuated appearance in sperm ducts (Wolf et al 2004).…”

“…1, 10, 100 and 1000 µg/l, was achieved by means of a continuous flow system in the water of the aquariums. These doses are very similar to those previously employed by different authors in diverse fish species (Ishibashi et al 2005;Mandich et al 2007;Villaneuve et al 2012;Molina et al 2013). In agreement with previously reported data, no mortalities occurred during the BPA exposure period (Hatef et al 2012;Villaneuve et al 2012), nor were there any differences with regard to the weight and length of the animals after the study period.…”

Interferentes endócrinos no meio ambiente: um estudo de caso em amostras de água in natura e efluente de estação de tratamento de esgotos da região metropolitana de São Paulo

“…A concentração anormal da Vtg em peixes vem sendo utilizado como um indicador para a detecção de estrogenicidade ou mesmo inibição da formação de Vtg (GIMENO et al, 1998a, GIMENO et al, 1998bSHILLING e WILLIAMS, 2000;HAHN et al, 2002;SOLÉ et al, 2002;VILLENEUVE et al, 2002;HENNIES et al, 2003;PORTER e JANZ, 2003;SOLÉ et al, 2003a;SOLÉ et al, 2003b; VAN DEL BELT et al, 2003;DENSLOW et al, 2004;HEMMING et al, 2004;JOBLING et al, 2004;LAVADO et al;LAZORCHAK e SMITH, 2004;ME CARBALLO et al, 2005;DINIZ et al, 2005a;DORABAWILA e GUPTA, 2005;LETCHER et al, 2005;MA et al, 2005;MATOZZO e MARIN, 2005;ANDERSEN et al, 2006;HINCK et al, 2006;HOLBECH et al, 2006;MITCHELMORE e RICE, 2006;PETTERSSON et al, 2006;REMPEL et al, 2006;TOKISHITA et al, 2006;130 ANDERSSON et al, 2007;BARSE et al, 2007a;BARSE et al, 2007b;CARGOUËT et al, 2007;FOSSI et al, 2007;GAGNÉ et al, 2007;HINCK et al, 2007;KRISTENSEN et al, 2007;Lv et al, 2007;MANDICH et al, 2007;TADA et al, 2007;…”

“…Recomenda-se, portanto o uso de grupos de controle -que não tenham contato com o meio em estudo e estejam isentos da presença de interferentes endócrinos -para validação do método final. É importante notar que algumas espécies não são tão sensíveis a determinadas concentrações de IE quanto à indução de Vtg (MAGALHÃES et al, 2004;HOLBESH et al, 2006 (GIMENO et al,1998a;GIMENO et al,1998b;VILLENEUVE et al,2002;SOLÉ et al, 2003, MA et al, 2005MITCHELMORE e RICE, 2006;BARSE et al, 2007;MANDICH et al, 2007) Figuras 11 e 12: Um dos aquários em que foram mantidas as carpas para teste e material de teste.…”