In vivo evaluation of toxicity and antiviral activity of polyrhodanine nanoparticles by using the chicken embryo model

Nazaktabar, Ahmad; Lashkenari, Mohammad Soleimani; Araghi, Atefeh; Ghorbani, Mohsen; Golshahi, Hannaneh

doi:10.1016/j.ijbiomac.2017.05.069

Cited by 18 publications

(21 citation statements)

References 30 publications

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“…We observed that increased zoospore concentration causes a decrease in survival time, and infection of embryos at a later embryonic day (14) showed delayed mortality, which agrees with the observations of Jacobsen et al 14 garding embryo death without signs of pythiosis. 17,25,29 In the experimental model of pythiosis in rabbits, the subcutaneous lesions and histopathological findings resemble those observed in horses and other animals presenting the cutaneous form of pythiosis. 9 Histologically, we observed that the oomycete proliferated by invading blood vessels and forming thrombi; these histological findings resemble those seen in human patients and experimentally infected mice.…”

Section: Discussionmentioning

confidence: 70%

“…The inoculum dose of 50 zoospores/egg at 14th embryonic day was sufficient to cause 100% of mortality of the embryos. In this way, the chicken egg model can be useful to evaluate the anti‐ P. insidiosum activity of new drugs by the prevention of embryos mortality and, moreover, the toxicity can be evaluated regarding embryo death without signs of pythiosis …”

Section: Discussionmentioning

confidence: 99%

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Embryonated chicken eggs: An experimental model for Pythium insidiosum infection

Verdi

Jesus

Kommers

et al. 2017

Mycoses

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Pythiosis is a severe disease caused by Pythium insidiosum. Currently, the research on the treatment of pythiosis uses rabbits as an experimental infection model. To reduce the use of animals in scientific experimentation, alternative models are increasingly necessary options. The objective of this study was to establish a new experimental infection model for pythiosis using embryonated chicken eggs. First, we tested the inoculation of 4 zoospore concentrations into the egg allantoic cavity at 3 embryonic days. We observed that increased zoospore concentration causes a decrease in survival time, and at a later embryonic day (the 14th) of infection, embryos showed delayed mortality. To confirm the reproducibility of the model, we chose the 14th embryonic day for the inoculation of 50 zoospores/egg, and the experiment was repeated twice. Mortality began with 30% embryos 48 hours after inoculation, and 95% embryos died within 72 hours. There was no mortality in the uninfected control group. The infection was confirmed by culture, PCR and histopathology. Immunohistochemistry confirmed the presence of hyphae in blood vessels in the umbilical cords in 95% of embryos and only 1 liver (5%). Our results suggest that embryonated eggs can be a very useful alternative infection model to study pythiosis.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Embryonated chicken eggs: An experimental model for Pythium insidiosum infection

Verdi

Jesus

Kommers

et al. 2017

Mycoses

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“…One earlier report showed that polyrhodanine nanoparticles have potent anti-NDV activity in ovo, suggesting that this non-toxic material could be used in the control of NDV in chickens, as it reduced the egg infective dose 50 (EID 50 ) of the NDV strains isolated from outbreaks in Tehran, Iran, in 2009 [75]. Interestingly, egg embryos inoculated with 0.1, 1, 10, and 100 parts per million (ppm) of polyrhodanine had no pathological tissue lesions, abnormalities, or deformities, and there were also no changes in blood serum biochemical parameters [75].…”

Section: Newcastle Disease Virus (Ndv)mentioning

confidence: 99%

Nanoparticles as a novel and promising antiviral platform in veterinary medicine

et al. 2021

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Traditional veterinary virus vaccines, such as inactivated and live-attenuated vaccines, have achieved tremendous success in controlling many viral diseases of livestock and chickens worldwide. However, many recent viral outbreaks caused by different emerging and re-emerging viruses continue to be reported annually worldwide. It is therefore necessary to develop new control regimens. Nanoparticle research has received considerable attention in the last two decades as a promising platform with significant success in veterinary medicine, replacing traditional viral vector vaccines. However, the field of nanoparticle applications is still in its initial phase of growth. Here, we discuss various preparation methods, characteristics, physical properties, antiviral effects, and pharmacokinetics of well-developed nanoparticles and the potential of nanoparticles or nano-vaccines as a promising antiviral platform for veterinary medicine.

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“…80 The use of nanomaterials based on antiviral activity provided inactivation of some viruses in chicken embryo models and shrimp samples. 42 , 81 , 82 These studies have shown that nanotechnological approaches should be considered for the purpose of developing resistance against SARS-CoV-2. 41 , 42 , 43 , 83 Antimicrobial coatings by metal oxide nanostructures and nanopolymers may inhibit the transmission of COVID-19.…”

Section: Limitation Of Sars-cov-2 Spreadmentioning

confidence: 99%

An overview of food safety and COVID-19 infection

Ceylan

Ocak

Uçar

et al. 2021

Environmental and Health Management of Novel Coronavirus Disease (COVID-19 )

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In vivo evaluation of toxicity and antiviral activity of polyrhodanine nanoparticles by using the chicken embryo model

Cited by 18 publications

References 30 publications

Embryonated chicken eggs: An experimental model for Pythium insidiosum infection

Embryonated chicken eggs: An experimental model for Pythium insidiosum infection

Nanoparticles as a novel and promising antiviral platform in veterinary medicine

An overview of food safety and COVID-19 infection

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