In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Tolentino, Flora; Araújo, Priscila Alves de; Marques, Eduardo de Souza; Petreanu, Marcel; Andrade, Sérgio Faloni de; Niero, Rivaldo; Perazzo, Fábio Ferreira; Rosa, Paulo; Maistro, Edson Luis

doi:10.1016/j.jep.2015.02.013

Cited by 18 publications

(10 citation statements)

References 31 publications

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“…The results obtained did not show any mutagenic effects or cytotoxicity against Caco-2 cells (Kreander et al, 2006). Tolentino et al (2015) demonstrated the absence of genotoxic and mutagenic effects in a Rubus niveus extract (500, 1000, and 2000 mg/kg) using a comet assay. The results described above are in agreement with the results obtained in our study in which the absence of genotoxic, mutagenic, and cytotoxic effects for R. coriifolius were also verified.…”

Section: Genotoxicity (In Vivo and In Vitro)mentioning

confidence: 76%

Absence of toxicity and genotoxicity in an extract of Rubus coriifolius

et al. 2016

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Rubus coriifolius Focke is a wild plant from the Rosaceae family. It grows in both Guatemala and Mexico. The polar extract of the aerial parts of this plant has antibacterial, anti-inflammatory, and anti-protozoal activities. These properties may explain the traditional use of this plant. In vivo and in vitro assays were used to assess the genotoxic and toxic effects of an ethanol extract of the aerial parts of R. coriifolius. Three groups of rats were orally administered the R. coriifolius extract diluted in ethanol (5%) at doses of 1.89 mg/kg body weight (low dose), 4.72 mg/kg body weight (medium dose), and 9.44 mg/kg body weight (high dose) for 3 weeks. Genotoxic/cytotoxic effects induced by the R. coriifolius ethanol extract were evaluated in vivo by a micronuclei (MN) test in rat's bone marrow cells and in vitro by MN and sister chromatid exchange (SCE) in human lymphocyte cultures. In vivo genotoxicity analyses revealed that the average number of micronucleated polychromatic erythrocytes and the polychromatic erythrocyte/red blood cell ratio at all doses were not significantly different from those of the negative control. In vitro genotoxicity analyses showed that MN, SCE, and proliferative index frequencies in a human lymphocyte cell culture were not significantly different from those of the negative control. These results demonstrate that the ethanol extract of R. coriifolius aerial parts is not toxic or mutagenic (in vitro and in vivo) and does not affect cell proliferation at the concentrations analyzed.

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Section: Genotoxicity (In Vivo and In Vitro)mentioning

confidence: 76%

Absence of toxicity and genotoxicity in an extract of Rubus coriifolius

et al. 2016

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“…Despite papers demonstrate that the tannins have antioxidant effects [51][52][53][54], other studies also show that the tannins hinder the absorption of iron, acting as chelating [50,[55][56][57][58][59]. Thus, the reduction of bioavailable iron will be translated into a low oxygen tension in the blood [18], and it is known that low oxygen tension signals to produce erythropoietin, stimulating erythropoiesis [60][61].…”

Section: Discussionmentioning

confidence: 99%

“…Due to these characteristics the micronucleus assay in rodent bone marrow has long been used in the analysis of genotoxicity for many herbal medicines, especially those of pharmaceutical interest. Highlights are the analysis of Boriollo et al [14] with Ziziphus joazeiro Martius; Mahon et al [15] with Dilodendron bipinnatum Radlk; Oliveira et al [16] with Calophyllum brasiliense; Shin et al [17] with Polygala tenuifolia and Tolentino et al [18] with Rubus niveus.…”

Section: Introductionmentioning

confidence: 99%

Study of acute genotoxic potential of an aqueous extract of Schinus terebinthifolius Raddi: an in vivo micronucleus assay

Terra¹,

Maldonado²,

Alfradique³

et al. 2015

asb

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The in vivo Micronucleus Assay is a genotoxicity assessment system used for testing chemicals and herbal medicines that can induce chromosomal damage, acting as a biomarker. Schinus terebinthifolius Raddi (Anacardiaceae) is an evergreen tree that occurs on the Brazilian coast and several papers have shown the use of this plant in therapeutic approaches. The aim of this study is to analyze the genotoxic potential of Schinus terebinthifolius Raddi extract in Wistar rats bone marrow through the frequency of micronuclei in polychromatic erythrocytes. The results indicate that there was no genotoxic effects in all tested concentration, showing only an increase in the ratio of polychromatic and normochromatic erythrocytes.

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“…Some Rubus species are used in folk medicine to treat different diseases, particularly diabetes [6][7][8]. Phytochemical and pharmacological studies have shown that some Rubus species exert gastroprotective, antimicrobial and cytotoxic effects; no genotoxic effect was observed [9][10][11][12][13][14]. In our previous studies, we demonstrated the presence of terpenoids, which exhibited important antibacterial and antinociceptive activities [15].…”

mentioning

confidence: 86%

Extraction Optimization of 5,7-Dihydroxy-6,8,4′-trimethoxyflavonol, a Bioactive Flavonoid from Rubus rosifolius (Rosaceae) Leaves

Pittarello

Petreanu

Filho

et al. 2019

Natural Product Communications

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Recently, it has been demonstrated that a rare flavonoid, 5,7-dihydroxy-6,8,4ʹ-trimethoxyflavonol (1), isolated from the leaves of Rubus rosifolius showed significant antiproliferative activity against different cancer cell lines and had a diuretic effect. This study aimed to determine the best extraction method evaluating dynamic maceration extraction (DME), ultrasonic assisted extraction (UAE), and microwave assisted extraction (MAE). In addition, level optimization for different factors was focused using Design of Experiments. The flavonoid content was estimated by high performance liquid chromatography (HPLC). The results indicated that UAE provided the highest content with the shortest extraction time. For optimization, response surface methodology (RSM) was used, applying a Box-Behnken Design (BBD) with three factors at three levels. It was possible to observe that the flavonoid content significantly increased with the increase of temperature (max. 70ºC). The time had little influence on the extraction and, for this reason, was set at 25 min. With regard to the drug/solvent ratio, it was observed that this had an impact on yield with a ratio of 3.5%. A model was built and a determination coefficient of 0.98 was obtained. After validation, an optimized method was developed, able to extract 1 with a lower time, lower solvent consumption, and affording higher contents.

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In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Cited by 18 publications

References 31 publications

Absence of toxicity and genotoxicity in an extract of Rubus coriifolius

Absence of toxicity and genotoxicity in an extract of Rubus coriifolius

Study of acute genotoxic potential of an aqueous extract of Schinus terebinthifolius Raddi: an in vivo micronucleus assay

Extraction Optimization of 5,7-Dihydroxy-6,8,4′-trimethoxyflavonol, a Bioactive Flavonoid from Rubus rosifolius (Rosaceae) Leaves

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