In vivo evaluation of demyelination and remyelination in a nerve crush injury model

Bélanger, Erik; Henry, Francis P.; Vallée, Réal; Randolph, Mark A.; Kochevar, Irene E.; Winograd, Jonathan M.; Lin, Charles P.; Côté, Daniel

doi:10.1364/boe.2.002698

Cited by 70 publications

(64 citation statements)

References 23 publications

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“…CARS imaging of spinal tissue has been first reported in 2005 by Wang et al [11]. Since then, it has been used to visualize demyelination [12][13][14][15][16], and we have proposed several techniques to characterize myelin morphology in this context [17,18]. We now extend this work using CARS images of myelin acquired in mice affected with EAE to quantify the local tissue architecture within an image, but not the structure of individual myelin sheaths.…”

Section: Introductionmentioning

confidence: 88%

Local assessment of myelin health in a multiple sclerosis mouse model using a 2D Fourier transform approach

Bégin

Bélanger

Laffray

et al. 2013

Biomed. Opt. Express

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Abstract:We present an automated two-dimensional Fourier transform (2D-FT) approach to analyze the local organization of myelinated axons in the spinal cord. Coherent anti-Stokes Raman scattering (CARS) microscopy was used to observe lesions in a commonly used animal model of multiple sclerosis (MS), experimental autoimmune encephalomyelitis (EAE). A 2D-FT was applied on the CARS images to find the average orientation and directional anisotropy of the fibers within contiguous image domains. We introduce the corrected correlation parameter (CCP), a measure of the correlation between orientations of adjacent domains. We show that in the EAE animal model of MS, the CCP can be used to quantify the degree of organization/disorganization in the myelin structure. This analysis was applied to a large image dataset from animals at different clinical scores and we show that some descriptors of the CCP probability density function are strongly correlated with the clinical scores. This procedure, compatible with live animal imaging, has been developed to perform local in situ evaluation of myelinated axons afflicted by EAE. Anal. Chem. 36, 1627-1639 (1964). 29. P. L. Rosin, "Measuring shape: ellipticity, rectangularity, and triangularity," Mach. Vis. Appl. 14, 172-184 (2003). 30. J. Flusser and T. Suk, "Pattern recognition by affine moment invariants," Pattern Recogn. 26, 167-174 (1993).

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Section: Introductionmentioning

confidence: 88%

Local assessment of myelin health in a multiple sclerosis mouse model using a 2D Fourier transform approach

Bégin

Bélanger

Laffray

et al. 2013

Biomed. Opt. Express

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“…Numerous imaging modalities have been used to image peripheral nerves: ultrasound, 4,5 magnetic resonance imaging (MRI), 6,7 coherent anti-Stokes Raman spectroscopy (CARS), [8][9][10] and third-harmonic generation (THG) microscopy. 11,12 These imaging techniques enabled visualization of individual peripheral nerves in the body and assisted functional studies of nerves.…”

Section: Introductionmentioning

confidence: 99%

Label-free photoacoustic microscopy of peripheral nerves

et al. 2014

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Abstract. Peripheral neuropathy is a common neurological problem that affects millions of people worldwide. Diagnosis and treatment of this condition are often hindered by the difficulties in making objective, noninvasive measurements of nerve fibers. Photoacoustic microscopy (PAM) has the ability to obtain high resolution, specific images of peripheral nerves without exogenous contrast. We demonstrated the first proof-of-concept imaging of peripheral nerves using PAM. As validated by both standard histology and photoacoustic spectroscopy, the origin of photoacoustic signals is myelin, the primary source of lipids in the nerves. An extracted sciatic nerve sandwiched between two layers of chicken tissue was imaged by PAM to mimic the in vivo case. Ordered fibrous structures inside the nerve, caused by the bundles of myelin-coated axons, could be observed clearly. With further technical improvements, PAM can potentially be applied to monitor and diagnose peripheral neuropathies.

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“…Adapting polarized structural imaging for in vivo imaging is accessible, as shown already by in vivo CARS imaging [36]; however, it would necessitate the implementation of epi-detection modalities. To explore this direction, we have tested the fastpolarization CARS methodology in the epi mode, demonstrating very similar results as in forward detection (see Supplement 1, Fig.…”

Section: Discussionmentioning

confidence: 99%

High-speed polarization-resolved coherent Raman scattering imaging

Hofer¹,

Balla²,

Brasselet³

2017

Optica

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Polarization-resolved coherent Raman scattering (polar-CRS) provides rich information on molecular orientational organization, with the strong advantages of being a label-free and chemically specific imaging method. Its implementation, however, strongly reduces the imaging acquisition rate, due to limits imposed by polarization tuning. Here we demonstrate fast-polar-CRS imaging based on combined electro-optic polarization and acousto-optic amplitude modulations, applicable to both stimulated Raman scattering and coherent anti-Stokes Raman scattering imaging. The proposed scheme adds polarization information without compromising the capacities of regular CRS intensity imaging; increases the speed of orientational imaging by two orders of magnitude as compared with previous approaches; and does not require post-processing analyses. We show that this method permits sub-second time-scale imaging of lipid order packing and local lipid membrane deformations in artificial lipid multilayers, but also in red blood cell ghosts, demonstrating its high sensitivity down to a single lipid bilayer membrane.

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In vivo evaluation of demyelination and remyelination in a nerve crush injury model

Cited by 70 publications

References 23 publications

Local assessment of myelin health in a multiple sclerosis mouse model using a 2D Fourier transform approach

Local assessment of myelin health in a multiple sclerosis mouse model using a 2D Fourier transform approach

Label-free photoacoustic microscopy of peripheral nerves

High-speed polarization-resolved coherent Raman scattering imaging

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