Label-free photoacoustic microscopy of peripheral nerves

Matthews, Thomas P.; Zhang, Chi; Yao, Da-Kang; Maslov, Konstantin; Wang, Lihong V.

doi:10.1117/1.jbo.19.1.016004

Cited by 68 publications

(61 citation statements)

References 22 publications

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“…Photoacoustic elastography is well suited for mapping the elastic properties of diseased tissues with highly vascularized structures, such as carcinoma and glioblastoma [23]. At longer wavelengths where water and lipids have relatively strong absorption, photoacoustic elastography can potentially map the elastic properties of tissues by using water and lipids as the contrast [24–25]. …”

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confidence: 99%

Photoacoustic elastography

et al. 2016

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Elastography can noninvasively map the elasticity distribution in biological tissue, which can potentially be used to reveal disease conditions. In this paper, we have demonstrated photoacoustic elastography by using a linear-array photoacoustic computed tomography system. The feasibility of photoacoustic elastography was first demonstrated by imaging the strains of single-layer and bilayer gelatin phantoms with various stiffness values. The measured strains agreed well with the theoretical values, with an average error of less than 5.2%. Next, in vivo photoacoustic elastography was demonstrated on a mouse leg, where the fat and muscle distribution was mapped based on the elasticity contrast. We confirmed the photoacoustic elastography results by ultrasound elastography performed simultaneously.

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confidence: 99%

Photoacoustic elastography

et al. 2016

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“…Therefore, OR-PAM can label-freely image microvasculature at the capillary level based on the intrinsic optical absorption from hemoglobin; in addition, using spectroscopic photoacoustic measurement, it can spectrally identify a variety of endogenous and/or exogenous chromophores [7][8][9]. To date, OR-PAM has successfully demonstrated the capability of providing optical-absorption-based anatomic, functional, and molecular information, and found broad applications in vascular biology [10], neurology [11], and ophthalmology [12].…”

Section: Introductionmentioning

confidence: 99%

Reflection-mode in vivo photoacoustic microscopy with subwavelength lateral resolution

Song¹,

Zheng²,

Liu³

et al. 2014

Biomed. Opt. Express

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Abstract:We developed a reflection-mode subwavelength-resolution photoacoustic microscopy system capable of imaging optical absorption contrast in vivo. The simultaneous high-resolution and reflection-mode imaging capacity of the system was enabled by delicately configuring a miniature high-frequency ultrasonic transducer tightly under a water-immersion objective with numerical aperture of 1.0. At 532-nm laser illumination, the lateral resolution of the system was measured to be ~320 nm. With this system, subcellular structures of red blood cells and B16 melanoma cells were resolved ex vivo; microvessels, including individual capillaries, in a mouse ear were clearly imaged label-freely in vivo, using the intrinsic optical absorption from hemoglobin. The current study suggests that, the optical-absorption contrast, subwavelength resolution, and reflection-mode ability of the developed photoacoustic microscopy may empower a wide range of biomedical studies for visualizing cellular and/or subcellular structures.

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“…An electrophysiological testing method such as electromyographic (EMG) stimulation [11][12][13] and various imaging methods have been developed and are under investigation to provide better identification of CNs [14][15][16][17]. The imaging methods are transrectal ultrasound (TRUS) [18][19][20][21], dye-based fluorescence imaging [22][23][24][25][26][27][28][29][30], multiphoton microscopy (MPM) [31,32], coherent anti-Stokes Raman scattering (CARS) microscopy [33], and photoacoustic microscopy (PAM) [34,35]. However, these imaging methods have their own limitations as far as their use for the surgery.…”

Section: Introductionmentioning

confidence: 99%

Visualization of prostatic nerves by polarization-sensitive optical coherence tomography

Yoon

Jeon

Park

et al. 2016

Biomed. Opt. Express

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Preservation of prostatic nerves is critical to recovery of a man's sexual potency after radical prostatectomy. A real-time imaging method of prostatic nerves will be helpful for nerve-sparing radical prostatectomy (NSRP). Polarization-sensitive optical coherence tomography (PS-OCT), which provides both structural and birefringent information of tissue, was applied for detection of prostatic nerves in both rat and human prostate specimens, ex vivo. PS-OCT imaging of rat prostate specimens visualized highly scattering and birefringent fibrous structures superficially, and these birefringent structures were confirmed to be nerves by histology or multiphoton microscopy (MPM). PS-OCT could easily distinguish these birefringent structures from surrounding other tissue compartments such as prostatic glands and fats. PS-OCT imaging of human prostatectomy specimens visualized two different birefringent structures, appearing fibrous and sheet-like. The fibrous ones were confirmed to be nerves by histology, and the sheet-like ones were considered to be fascias surrounding the human prostate. PS-OCT imaging of human prostatectomy specimens along the perimeter showed spatial variation in the amount of birefringent fibrous structures which was consistent with anatomy. These results demonstrate the feasibility of PS-OCT for detection of prostatic nerves, and this study will provide a basis for intraoperative use of PS-OCT. References and links1. P. C. Walsh and P. J. Donker, "Impotence following radical prostatectomy: insight into etiology and prevention," J. Urol. 128(3), 492-497 (1982). 2. F. Montorsi, A. Salonia, N. Suardi, A. Gallina, G. Zanni, A. Briganti, F. Deho', R. Naspro, E. Farina, and P.Rigatti, "Improving the preservation of the urethral sphincter and neurovascular bundles during open radical retropubic prostatectomy," Eur. Urol. 48(6), 938-945 (2005 524-535 (2015).

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Label-free photoacoustic microscopy of peripheral nerves

Cited by 68 publications

References 22 publications

Photoacoustic elastography

Photoacoustic elastography

Reflection-mode in vivo photoacoustic microscopy with subwavelength lateral resolution

Visualization of prostatic nerves by polarization-sensitive optical coherence tomography

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