In Vivo Evaluation of Antirrhinum majus’ Wound-Healing Activity

Abstract

Mediterranean-native perennial plant Antirrhinum majus was scrutinized in this study for its antioxidant activity and its total phenolic content in order to test for the plant’s wound-healing capability. The traditional uses of this plant to treat gum scurvy, various tumors, ulcers, and hemorrhoids were the main idea behind this study. Leaves and flowers of the A. majus were extracted by maceration. Pilot qualitative phytochemical tests were made to check the presence of various secondary metabolites. Quantitatively, the flowers’ macerate indicated superlative results regarding antioxidant activity and total phenolic content. However, the in vivo wound-healing capability study was made using 30 Wistar strain albino rats. This innovative part of the study revealed that the healing power of the flowers’ extract ointment (5% w/w) was superior compared to the leaves’ extract (5% w/w) and the positive-control ointments (MEBO) (1.5% w/w) (p ≤ 0.001). This activity was assessed by visual examination, wound-length measurement, and estimation of hydroxyproline content. Antirrhinum majus is a promising plant to be considered for wound healing. However, further testing (including histological examination and high-performance liquid chromatography (HPLC) analysis) is necessary to understand more about its mechanisms of action.

Membrana de cáscara de huevo para la curación de heridas superficiales en ratones

“…En este estudio, se evaluó el uso de las membranas de huevo aplicadas de dos formas, directamente o en polvo, para promover la cicatrización de heridas superficiales por incisión longitudinal en ratón 28 . Se recurrió a este modelo porque este tipo de herida es más corriente en la vida cotidiana que las heridas por escisión 2 , 28 .…”

Evaluation of wound healing potential of a sea cucumber (Actinopyga mauritiana) extract in mice (Mus musculus)

“…The proliferation occurred from the 7th to the 14th post-injury and the healing process will continue until the skin structure returns to normal. Secondary metabolites such as steroids facilitated the anti-inflammatory effect [34], glycosides accelerated wound contraction [35], and flavonoids enhanced the number of fibroblasts [36]. Fibroblasts proliferation is stimulated by interleukin-Ib (IL-Ib), fibroblast growth factor (FGF), and platelet-derived growth factor (PDGF) [37].…”

Figure 1 from: Mahmod AI, Talib WH (2021) Anticancer activity of Mandragora autumnalis: an in vitro and in vivo study. Pharmacia 68(4): 827-835. https://doi.org/10.3897/pharmacia.68.e71695

“…Various types of active chemical constituents such as tannins, terpenoids, alkaloids, phenols, flavonoids, and steroids were detected using qualitative chemical tests. Identification of the extracted components was achieved according to the standard methods described by Saqallah et al (Saqallah et al 2018).…”