Membrana de cáscara de huevo para la curación de heridas superficiales en ratones

Mendoza, Noelia; Chávez, Georgina; Araya, Omar

doi:10.7705/biomedica.6192

Cited by 4 publications

(2 citation statements)

References 20 publications

(30 reference statements)

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“…The ESM also contains fibronectin which in addition to the arginine-glycine aspartic acid tripeptide glycoprotein motif facilitates cell adhesion. Meanwhile the fibronectin also facilitates cell growth, migration and repair which play a key role in wound healing and cell incorporation to ensure the success of the scaffold (Scatena et al 2007, Mensah et al 2021, Mendoza et al 2022. Furthermore, the presence of osteopontin in the ESM facilitates tissue repair and remodelling while also regulating cytokine release and macrophage recruitment (Scatena et al 2007, Han et al 2023.…”

Section: Biological Propertiesmentioning

confidence: 99%

The chicken eggshell membrane: a versatile, sustainable, biological material for translational biomedical applications

et al. 2023

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Naturally derived materials are often preferred to than synthetic materials for biomedical applications due to their innate biological characteristics, relative availability, sustainability, and agreement with conscientious end-users. The chicken eggshell membrane (ESM) is an abundant resource with a defined structural profile, chemical composition, and validated morphological and mechanical characteristics. These unique properties have not only allowed the ESM to be exploited within the food industry, but has also led to it be considered for other novel translational applications such as tissue regeneration and replacement, filtration aids and barrier devices, and environmental health engagement. However, challenges still exist in order to enhance the native ESM: the need to improve its mechanical properties, the ability to combine/join fragments of ESM together, and the addition or incorporation of drugs/growth factors to advance its therapeutic capacity. This review article provides a succinct background to the native ESM, its extraction, isolation, and consequent physical, mechanical and biological characterisation including possible approaches to enhancement. Moreover, it also highlights current applications of the ESM in regenerative medicine and hints at future novel applications in which this novel biomaterial could be exploited to beneficial use.

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Section: Biological Propertiesmentioning

confidence: 99%

The chicken eggshell membrane: a versatile, sustainable, biological material for translational biomedical applications

et al. 2023

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“…Likewise, the high surface area of the ESM lets it serve as an adsorbent of drugs, nanoparticles, or microparticles to improve tissue regeneration via controlled drug release [ 7 , 9 ]. Based on these characteristics, the ESM have been exploited as a potential biomaterial for dermal wound-healing applications [ 7 , 9 , 11 , 12 , 13 , 14 , 15 ]. One important process for the use of ESM as a biomaterial is its separation from the eggshell (ES).…”

Section: Introductionmentioning

confidence: 99%

A Sustainable, Green-Processed, Ag-Nanoparticle-Incorporated Eggshell-Derived Biomaterial for Wound-Healing Applications

Mensah,

Trotta,

Briggs

et al. 2023

JFB

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The eggshell membrane (ESM) is a natural biomaterial with unique physical and mechanical properties that make it a promising candidate for wound-healing applications. However, the ESM’s inherent properties can be enhanced through incorporation of silver nanoparticles (AgNPs), which have been shown to have antimicrobial properties. In this study, commercially produced AgNPs and green-processed AgNPs were incorporated into ESM and evaluated for their physical, biological, and antimicrobial properties for potential dermal application. The ESM was extracted using various techniques, and then treated with either commercially produced AgNPs (Sigma-Aldrich, Poole, UK) or green-synthesized AgNPs (Metalchemy, London, UK) to produce AgNPs-ESM samples. The physical characteristics of the samples were evaluated using scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, and the biological properties were assessed through in vitro studies using human dermal fibroblasts (HDFs) and BJ cells. The SEM analysis of the AgNPs-ESM samples showed localization of AgNPs on the ESM surface, and that the ESM maintained its structural integrity following AgNP incorporation. The FTIR confirmed loading of AgNPs to ESM samples. The biological studies showed that the 5 μg/mL AgNPs-ESM samples were highly biocompatible with both HDFs and BJ cells, and had good viability and proliferation rates. Additionally, the AgNPs-ESM samples demonstrated pro-angiogenic properties in the CAM assay, indicating their potential for promoting new blood vessel growth. Assessment of the antimicrobial activity of the enhanced AgNPs/ESMs was validated using the International Standard ISO 16869:2008 methodology and exploited Cladosporium, which is one of the most commonly identified fungi in wounds, as the test microorganism (≥5 × 106 cells/mL). The AgNPs-ESM samples displayed promising antimicrobial efficacy as evidenced by the measured zone of inhibition. Notably, the green-synthesized AgNPs demonstrated greater zones of inhibition (~17 times larger) compared to commercially available AgNPs (Sigma-Aldrich). Although both types of AgNP exhibited long-term stability, the Metalchemy-modified samples demonstrated a slightly stronger inhibitory effect. Overall, the AgNPs-ESM samples developed in this study exhibited desirable physical, biological, and antimicrobial properties for potential dermal wound-dressing applications. The use of green-processed AgNPs in the fabrication of the AgNPs-ESM samples highlights the potential for sustainable and environmentally friendly wound-healing therapies. Further research is required to assess the long-term biocompatibility and effectiveness of these biomaterials in vivo.

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Eggshell membrane: Structure, purification, properties and multifunctional applications

Liang,

Cong,

Jiang

et al. 2024

Food Bioscience

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Membrana de cáscara de huevo para la curación de heridas superficiales en ratones

Cited by 4 publications

References 20 publications

The chicken eggshell membrane: a versatile, sustainable, biological material for translational biomedical applications

The chicken eggshell membrane: a versatile, sustainable, biological material for translational biomedical applications

A Sustainable, Green-Processed, Ag-Nanoparticle-Incorporated Eggshell-Derived Biomaterial for Wound-Healing Applications

Eggshell membrane: Structure, purification, properties and multifunctional applications

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