In Vivo Evaluation of 3-Dimensional Polycaprolactone Scaffolds for Cartilage Repair in Rabbits

Martínez-Díaz, Santos; Garcia-Giralt, Natàlia; Lebourg, Myriam; Gómez-Tejedor, José A.; Vilà, G.; Cáceres, Enric; Benito, Pere; Pradas, Manuel Monleón; Nogués, Xavier; Ribelles, J.L. Gómez; Monllau, Joan Carles

doi:10.1177/0363546509352448

Cited by 101 publications

(100 citation statements)

References 50 publications

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“…11b). The repair of articular cartilage we observed agrees with previous studies where hyaline cartilage was regenerated on the top of the scaffolds, using similar protocol but with different biomaterials (30), suggesting that the fast growth of this tissue layer had embed the scaffold towards the subchondral bone. In fact, in the early stages after implantation we observe a large number of chondrocytes in many lacunae of the surrounding native cartilage, that later on synthesize cartilaginous matrix, originating the overall growth of the articular cartilage.…”

Section: Articular Cartilage Repairsupporting

confidence: 89%

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Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

Sancho‐Tello

Forriol²,

Gastaldi³

et al. 2015

Int J Artif Organs

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The Authors state that this manuscript has not been published previously and is not currently being assessed for publication by any journal other than The InternationalJournal of Artificial Organs.Each Author has contributed substantially to the research, preparation and production of the paper and approves of its submission to the Journal. FINANCIAL SUPPORT CONFLICT OF INTERESTThe authors state they do not have proprietary interest. MEETING PRESENTATIONThis study has been partially presented at the following meeting: ABSTRACT Purpose: Tissue engineering techniques were used to study cartilage repair over 12-month period in a rabbit model. Methods:A full-depth chondral defect along with subchondral bone injury were originated in the knee joint, where a biostable porous scaffold was implanted, synthesized of poly(ethyl acrylate-co-hydroxyethyl acrylate) copolymer. Morphological evolution of cartilage repair was studied 1 and 2 weeks, and 1, 3 and 12 months after implantation by histological techniques. Three-month group was chosen to compare cartilage repair to an additional group where scaffolds were preseeded with allogeneic chondrocytes before implantation, and also to controls, who underwent the same surgery procedure, with no scaffold implantation.

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Section: Articular Cartilage Repairsupporting

confidence: 89%

“…Although rabbit knee articular cartilage presents some morphological and biomechanical differences with respect to human, it has biological aspects similar to human, and thus the model is considered as suitable for our study (30).…”

Section: Discussionmentioning

confidence: 99%

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

Sancho‐Tello

Forriol²,

Gastaldi³

et al. 2015

Int J Artif Organs

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“…Such structure favors scaffold permeability to nutrients and waste products of cell metabolism and can be used to retain active components 11,33,34 . However, apparent scaffold stiffness becomes smaller than in similar sponges lacking microporosity 13,35 .…”

Section: Morphology Morphology Variation and Mechanical Responsementioning

confidence: 85%

In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

et al. 2014

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In vitro mechanical fatigue behavior of poly-ɛ-caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel AbstractPolymeric scaffolds used in regenerative therapies are implanted in the damaged tissue and submitted to repeated loading cycles. In the case of articular cartilage engineering, an implanted scaffold is typically subjected to long-term dynamic compression. The evolution of the mechanical properties of the scaffold during bioresorption has been deeply studied in the past, but the possibility of failure due to mechanical fatigue has not been properly addressed. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. In this work fatigue studies of polycaprolactone scaffolds were carried by subjecting the scaffold to repeated compression cycles in conditions simulating the scaffold implanted in the articular cartilage. The behavior of the polycaprolactone sponge with the pores filled with a poly(vinyl alcohol) gel simulating the new formed tissue within the pores was compared with that of the material immersed in water. Results were analyzed with Morrow's criteria for failure and accurate fittings are obtained just up to 200 loading cycles. It is also shown that the presence of poly(vinyl alcohol) increases the elastic modulus of the scaffolds, the effect being more pronounced with increasing the number of freeze/thawing cycles. AbstractPolymeric scaffolds used in regenerative therapies are implanted in the damaged tissue and submitted to repeated loading cycles. In the case of articular cartilage engineering, an implanted scaffold is typically subjected to long term dynamic compression. The evolution of the mechanical properties of the scaffold during bioresorption has been deeply studied in the past, but the possibility of failure due to mechanical fatigue has not been properly addressed.Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. In this work fatigue studies of polycaprolactone scaffolds were carried by subjecting the scaffold to repeated compression cycles in conditions simulating the scaffold implanted in the articular cartilage. The behaviour of the polycaprolactone sponge with the pores filled with a poly(vinyl alcohol) gel simulating the new formed tissue within the pores was compared with that of the material immersed in water. Results were analyzed with Morrow's criteria for failure 2 and accurate fittings are obtained just up to 200 loading cycles. It is also shown that the presence of poly(vinyl alcohol) increases the elastic modulus of the scaffolds, the effect being more pronounced with increasing the number of freeze/thawing cycles.

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“…In previous work authors have conducted indentation studies of the articular cartilage of rabbits [30,32].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

An “in vitro” experimental model to predict the mechanical behavior of macroporous scaffolds implanted in articular cartilage

Vikingsson

Ferrer

Gómez-Tejedor

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

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A model is proposed to assess mechanical behaviour of tissue engineering scaffolds and predict their performance "in vivo" during tissue regeneration. To simulate the growth of tissue inside the pores of the scaffold, the scaffold is swollen with a Poly (Vinyl alcohol) solution and subjected to repeated freezing and thawing cycles. In this way the Poly (Vinyl alcohol) becomes a gel whose stiffness increases with the number of freezing and thawing cycles. Mechanical properties of the construct immersed in water are shown to be determined, in large extent, by the water mobility constraints imposed by the gel filling the pores. This is similar to the way that water mobility determines mechanical properties of highly hydrated tissues, such as articular cartilage. As a consequence, the apparent elastic modulus of the scaffold in compression tests is much higher than those of the empty scaffold or the gel. Thus this experimental model allows assessing fatigue behaviour of the scaffolds under long-term dynamic loading in a realistic way, without recourse to animal experimentation. L. Vikingsson, et al., J Mech Behav Biomed 32 (2014) 125-131 2

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In Vivo Evaluation of 3-Dimensional Polycaprolactone Scaffolds for Cartilage Repair in Rabbits

Cited by 101 publications

References 50 publications

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

Time Evolution of in Vivo Articular Cartilage Repair Induced by Bone Marrow Stimulation and Scaffold Implantation in Rabbits

In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

An “in vitro” experimental model to predict the mechanical behavior of macroporous scaffolds implanted in articular cartilage

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