<i>In vitro</i>mechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

Panadero, J. A.; Vikingsson, L.; Ribelles, J. L. Gomez; Lanceros-Méndez, S.; Sencadas, Vítor

doi:10.1002/jbm.b.33276

Cited by 14 publications

(14 citation statements)

References 35 publications

(57 reference statements)

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“…The elastic moduli after cell culture were also compared with data reported for PCL filled with a PVA gel, that hardens by freeze/thawing cycles and serves as mechanical model of growing tissue. 32,33 The elastic modulus after cell culture is similar to the highest values obtained using PCL1PVA with six cycles of freeze-thawing. No statistical difference in elastic modulus is observed between cell culture samples under free-swelling and cyclic loading conditions.…”

Section: Mechanical Behaviorsupporting

confidence: 69%

“…Both PCL+Fibrin groups show a slight increase of the elastic modulus after cell cultivation. The elastic moduli after cell culture were also compared with data reported for PCL filled with a PVA gel, that hardens by freeze/thawing cycles and serves as mechanical model of growing tissue . The elastic modulus after cell culture is similar to the highest values obtained using PCL+PVA with six cycles of freeze‐thawing.…”

Section: Resultsmentioning

confidence: 68%

“…It was previously observed that the mechanical hysteresis of PCL scaffolds and its fatigue behavior is affected by the presence of water inside of porous structure, the main contribution of material fatigue behavior being given by aqueous media that acts as plasticizer and promotes a uniform distribution of the applied stress along the sample and not only in the trabeculae of PCL. 8 Moreover, the incorporation of fibrin without cells or even PVA with different freeze/thawing cycles inside of polymer porous does not influence the fatigue material performance 32 as much as the cell culture in different conditions does. Figure 4(b) shows that the mechanical stability of PCL scaffolds after cell seeding was increased up to 600 cycles, which is higher than the ones observed for the PCL, PCL 1 fibrin and PCL filled with PVA.…”

Section: Morrow Energy Model: Plastic Strain Energy Density-life Modelmentioning

confidence: 99%

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Mechanical fatigue performance of PCL‐chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

et al. 2015

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In tissue engineering of cartilage, polymeric scaffolds are implanted in the damaged tissue and subjected to repeated compression loading cycles. The possibility of failure due to mechanical fatigue has not been properly addressed in these scaffolds. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. This is related to inherent discontinuities in the material due to the micropore structure of the macropore walls that act as stress concentration points. In this work, chondrogenic precursor cells have been seeded in poly-e-caprolactone (PCL) scaffolds with fibrin and some were submitted to free swelling culture and others to cyclic loading in a bioreactor. After cell culture, all the samples were analyzed for fatigue behavior under repeated loading-unloading cycles. Moreover, some components of the extracellular matrix (ECM) were identified. No differences were observed between samples undergoing free swelling or bioreactor loading conditions, neither respect to matrix components nor to mechanical performance to fatigue. The ECM did not achieve the desired preponderance of collagen type II over collagen type I which is considered the main characteristic of hyaline cartilage ECM. However, prediction in PCL with ECM constructs was possible up to 600 cycles, an enhanced performance when compared to previous works. PCL after cell culture presents an improved fatigue resistance, despite the fact that the measured elastic modulus at the first cycle was similar to PCL with poly(vinyl alcohol) samples. This finding suggests that fatigue analysis in tissue engineering constructs can provide additional information missed with traditional mechanical measurements. AbstractIn tissue engineering of cartilage, polymeric scaffolds are implanted in the damaged tissue and subjected to repeated compression loading cycles. The possibility of failure due to mechanical fatigue has not been properly addressed in these scaffolds. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. This is related to inherent discontinuities in the material due to the micropore structure of the macro-pore walls that act as stress concentration points. In this work, chondrogenic precursor cells have been seeded in Poly-ε-caprolactone (PCL) scaffolds with fibrin and some were submitted to free swelling culture and others to cyclic loading in a bioreactor. After cell culture, all the samples were analyzed for fatigue behavior under repeated loading-unloading cycles. Moreover, some components of the extracellular matrix (ECM) were identified. No differences were observed 2 between samples undergoing free swelling or bioreactor loading conditions, neither respect to matrix components nor to mechanical performance to fatigue. The ECM did not achieve the desired preponderance of collagen type II over collagen type I which is considered the main characteristic of hyaline cartilage ECM. However, prediction in PCL with ECM constructs was possible u...

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Section: Mechanical Behaviorsupporting

confidence: 69%

Section: Resultsmentioning

confidence: 68%

Section: Morrow Energy Model: Plastic Strain Energy Density-life Modelmentioning

confidence: 99%

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Mechanical fatigue performance of PCL‐chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

et al. 2015

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“…35,36,37,26 Briefly: a 10% w/v aqueous solution of PVA was prepared by stirring at 80°C for 2 hours and left to cool to room temperature. The viscous solution was then poured 6 into a 48 multi-well cell culture plate.…”

Section: Scaffold/hydrogel Constructsmentioning

confidence: 99%

Local deformation in a hydrogel induced by an external magnetic field

et al. 2016

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“…The porosity of composite scaffolds has been maintained even after CHT and CHT-HA hydrogel impregnation (60.5 ± 1.1 and 57.8 ± 2.1%, respectively) indicating highly porous hydrogel structure. Considering other studies [27] where pores of synthetic scaffold have been fully impregnated and closed by hydrogel, we have achieved high porosity of hydrogels suitable for unhindered cell migration through whole scaffold's volume and better biocompatible properties.…”

Section: Estimated Porosity and Swellingmentioning

confidence: 94%

Macroporous poly(lactic acid) construct supporting the osteoinductive porous chitosan-based hydrogel for bone tissue engineering

Rogina

Pribolšan

Hanžek

et al. 2016

Polymer

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In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

Cited by 14 publications

References 35 publications

Mechanical fatigue performance of PCL‐chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

Mechanical fatigue performance of PCL‐chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

Local deformation in a hydrogel induced by an external magnetic field

Macroporous poly(lactic acid) construct supporting the osteoinductive porous chitosan-based hydrogel for bone tissue engineering

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