In vivo effect of hyperbaric oxygen on wound angiogenesis and epithelialization

Sander, Anna; Henrich, Dirk; Muth, Claus M.; Marzi, Ingo; Barker, John H.; Frank, Johannes

doi:10.1111/j.1524-475x.2009.00455.x

Cited by 73 publications

(51 citation statements)

References 30 publications

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“…Indeed, animals exposed to HBO exhibited skin revascularization in the lesion site which could facilitate glucantime delivery. Previous reports have shown in wound mouse models 27,31 , transplanted rats 31 , and rat liver after partial hepatectomy 17 that neovascularization is increased in animals exposed to HBO. To analyze whether the therapies induce immune responses, INF-γ and IL-4 levels were measured.…”

Section: Discussionmentioning

confidence: 94%

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

Ayres

Fedele

Marcucci

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIn this study we investigated the efficacy of hyperbaric oxygen (HBO) therapy, alone or combined with the pentavalent antimonial glucantime on Leishmania amazonensis infection. In parallel, the effect of Brazilian red propolis gel (propain) alone or combined with glucantime on L. amazonensis infection was evaluated. The inhibition of the infection in macrophages treated with glucantime in combination with HBO exposition was greater than that of macrophages treated with glucantime alone or HBO alone. The susceptible mouse strain BALB/c infected in the shaved rump with L. amazonensis treated with glucantime and exposed to HBO showed: time points in the course of the disease in which lesions were smaller than those of mice treated with glucantime alone and revascularization of the skin in the lesion site; interferon-gamma (IFN-γ) levels were not elevated in lymph node cells from these animals. Propain alone was not efficient against lesions, although less exudative lesions were observed in animals treated with propain alone or combined with glucantime. These results reveal the potential value of HBO and red propolis in combination with glucantime for treating cutaneous leishmaniasis and encourage further studies on the effect of more aggressive HBO, propolis and glucantime therapies on different mouse models of leishmaniasis.

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Section: Discussionmentioning

confidence: 94%

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

Ayres

Fedele

Marcucci

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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“…In fact, HBOT is widely used for the treatment of diabetic ulcers (Duzgun et al 2008;Liu and Velazquez 2008;Ong 2008;Thackham et al 2008;Efrati et al 2009), an application that will only increase as the population ages. One way HBOT acts to enhance chronic wound healing is by stimulating angiogenesis (Ren et al 2008;Milovanova et al 2009;Sander et al 2009;Yuan et al 2009). The tissue responsible for angiogenesis, the vascular endothelium, has been the topic of several studies to determine the mechanisms of the beneficial effects of HBO.…”

Section: Introductionmentioning

confidence: 98%

Hyperbaric oxygen induces a cytoprotective and angiogenic response in human microvascular endothelial cells

Godman

Chheda

Hightower

et al. 2010

Cell Stress and Chaperones

121

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A genome-wide microarray analysis of gene expression was carried out on human microvascular endothelial cells (HMEC-1) exposed to hyperbaric oxygen treatment (HBOT) under conditions that approximated clinical settings. Highly up-regulated genes included immediate early transcription factors (FOS, FOSB, and JUNB) and metallothioneins. Six molecular chaperones were also up-regulated immediately following HBOT, and all of these have been implicated in protein damage control. Pathway analysis programs identified the Nrf-2-mediated oxidative stress response as one of the primary responders to HBOT. Several of the microarray changes in the Nrf2 pathway and a molecular chaperone were validated using quantitative PCR. For all of the genes tested (Nrf2, HMOX1, HSPA1A, M1A, ACTC1, and FOS), HBOT elicited large responses, whereas changes were minimal following treatment with 100% O(2) in the absence of elevated pressure. The increased expression of immediate early and cytoprotective genes corresponded with an HBOT-induced increase in cell proliferation and oxidative stress resistance. In addition, HBOT treatment enhanced endothelial tube formation on Matrigel plates, with particularly dramatic effects observed following two daily HBO treatments. Understanding how HBOT influences gene expression changes in endothelial cells may be beneficial for improving current HBOT-based wound-healing protocols. These data also point to other potential HBOT applications where stimulating protection and repair of the endothelium would be beneficial, such as patient preconditioning prior to major surgery.

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“…Interestingly, in this study, it was concluded that part of the positive effect of wound outcome was due to the release of metalloproteinases [26]. This is not inconsistent with many published reports because their data showed that MMP-9 was elevated significantly in the impaired healing group.…”

Section: Discussionmentioning

confidence: 52%

Hyperbaric Oxygen Effect on MMP-9 After a Vascular Insult

Cummins

Gentene

2010

J. of Cardiovasc. Trans. Res.

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Matrix metalloproteinease-9 (MMP-9) is involved in a host of processes. Many of its processes are physiologically beneficial as well as detrimental. The over-expression of this enzyme has been implicated as a contributory factor to some of the sequalae associated with cerebral ischemia, cell death, non-healing wounds, traumatic brain injury, aneurysms, and plaque instability in atherosclerosis. Several studies have examined the effect of hyperbaric oxygen (HBO) on MMP-9 expression. Because this proteinase is involved in both chronic and acute pathology, we wanted to investigate an acute expression model and see if, and how quickly, its expression would respond to HBO therapy. Our patient was scheduled to have elective surgery with an overnight stay followed by a series of HBO exposures. The patient served as her own control. An MMP-9 and urine pH was obtained prior to surgery to establish a baseline. On days 1, 3, and 4 post-op, samples were obtained before and after hyperbaric exposure. The patient was exposed to 100% O2 at 2.5 ATA for 60 min during each treatment for 5 days. The patient's MMP-9 values were dramatically elevated after surgery as compared to the baseline readings. The percentage increase from baseline was 400%. Our patient showed a significant reduction in MMP-9 expression after each hyperbaric exposure with the greatest decrease seen on post-op day 1 and subsequent exposures showing slightly less expression. Reduction in MMP-9 expression ranged from 46% on day 1 to 30% on post-op day 4. This case study suggests that if done relatively soon after a vascular or tissue insult, HBO can reduce MMP-9 expression. Chronic vascular pathologies, such as atherosclerotic plaque and aneurysms where over-expression of MMP-9 may result in acute coronary syndrome (ACS) or cerebral vascular accidents (CVAs), may be mitigated by a series of HBO treatments that reduce MMP-9 expression. Causality and/or contributory effects of MMP-9 expression in both pathologic and physiologic processes needs to be further elucidated. The understanding of how HBO therapy modulates these may provide an additional insight into mechanisms and future potential therapies for pathologic conditions such as those described above.

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In vivo effect of hyperbaric oxygen on wound angiogenesis and epithelialization

Cited by 73 publications

References 30 publications

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime

Hyperbaric oxygen induces a cytoprotective and angiogenic response in human microvascular endothelial cells

Hyperbaric Oxygen Effect on MMP-9 After a Vascular Insult

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