In vivo DTI tractography of the rat brain: an atlas of the main tracts in Paxinos space with histological comparison

Zucca, Ileana; Aquino, Domenico; Pennacchio, Paolo; Nava, Simone; Marzio, Alessandro Di; Preti, Maria Giulia; Baselli, Guseppe; Spreafico, Roberto; Frassoni, Carolina

doi:10.1016/j.mri.2014.11.001

Cited by 27 publications

(19 citation statements)

References 51 publications

(72 reference statements)

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“…The visual appearance of T 2 B maps of the rat brain at 11.7T (Fig. 2) is in agreement with this finding, as slightly higher T 2 B values are observed in WM regions comprising fiber tracts that are known (Figini et al, 2015; Jugé et al, 2016) to run approximately parallel to the direction of the main magnetic field vector (rostral-caudal direction in Fig. 2).…”

Section: Discussionsupporting

confidence: 81%

High-resolution three-dimensional macromolecular proton fraction mapping for quantitative neuroanatomical imaging of the rodent brain in ultra-high magnetic fields

et al. 2017

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A well-known problem in ultra-high-field MRI is generation of high-resolution three-dimensional images for detailed characterization of white and gray matter anatomical structures. T1-weighted imaging traditionally used for this purpose suffers from the loss of contrast between white and gray matter with an increase of magnetic field strength. Macromolecular proton fraction (MPF) mapping is a new method potentially capable to mitigate this problem due to strong myelin-based contrast and independence of this parameter of field strength. MPF is a key parameter determining the magnetization transfer effect in tissues and defined within the two-pool model as a relative amount of macromolecular protons involved into magnetization exchange with water protons. The objectives of this study were to characterize the two-pool model parameters in brain tissues in ultra-high magnetic fields and introduce fast high-field 3D MPF mapping as both anatomical and quantitative neuroimaging modality for small animal applications. In vivo imaging data were obtained from four adult male rats using an 11.7T animal MRI scanner. Comprehensive comparison of brain tissue contrast was performed for standard R1 and T2 maps and reconstructed from Z-spectroscopic images two-pool model parameter maps including MPF, cross-relaxation rate constant, and T2 of pools. Additionally, high-resolution whole-brain 3D MPF maps were obtained with isotropic 170 μm voxel size using the single-point synthetic-reference method. MPF maps showed 3–6-fold increase in contrast between white and gray matter compared to other parameters. MPF measurements by the single-point synthetic reference method were in excellent agreement with the Z-spectroscopic method. MPF values in rat brain structures at 11.7T were similar to those at lower field strengths, thus confirming field independence of MPF. 3D MPF mapping provides a useful tool for neuroimaging in ultra-high magnetic fields enabling both quantitative tissue characterization based on the myelin content and high-resolution neuroanatomical visualization with high contrast between white and gray matter.

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Section: Discussionsupporting

confidence: 81%

High-resolution three-dimensional macromolecular proton fraction mapping for quantitative neuroanatomical imaging of the rodent brain in ultra-high magnetic fields

et al. 2017

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“…Second, DTI was performed postmortem. In our pilot study, we have tried the in vivo DTI echo‐planar imaging (EPI) technique which has been used in vivo at lower field strengths (Fig ini et al., 2015) in order to do consecutive scanning of each animal before euthanasia. It took 10 hr for in vivo image acquisition, which was advised by our MRI expert (prof. Ching‐Po Lin) to obtain acceptable image quality for analysis.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of ischemic preconditioning against neuronal apoptosis and dendritic injury in the hippocampus are age‐dependent

Lee

Yang

Lin

et al. 2020

Journal of Neurochemistry

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Ischemic preconditioning with non‐lethal ischemia can be protective against lethal forebrain ischemia. We hypothesized that aging may aggravate ischemic susceptibility and reduce brain plasticity against preconditioning. Magnetic resonance diffusion tensor imaging (DTI) is a sensitive tool to detect brain integrity and white matter architecture. This study used DTI and histopathology to investigate the effect of aging on ischemic preconditioning. In this study, adult and middle‐aged male Mongolian gerbils were subjected to non‐lethal 5‐min forebrain ischemia (ischemic preconditioning) or sham‐operation, followed by 3 days of reperfusion, and then lethal 15‐min forebrain ischemia. A 9.4‐Tesla MR imaging system was used to study DTI indices, namely fractional anisotropy (FA), mean diffusivity (MD), and intervoxel coherence (IC) in the hippocampal CA1 and dentate gyrus (DG) areas. In situ expressions of microtubule‐associated protein 2 (MAP2, dendritic marker protein) and apoptosis were also examined. The 5‐min ischemia did not cause dendritic and neuronal injury and any significant change in DTI indices and MAP2 in adult and middle‐aged gerbils. The 15‐min ischemia‐induced significant delayed neuronal apoptosis and early dendritic injury evidenced by DTI and MAP2 studies in both CA1 and DG areas with more severe injury in middle‐aged gerbils than adult gerbils. Ischemic preconditioning could improve neuronal apoptosis in CA1 area and dendritic integrity in both CA1 and DG areas with better improvement in adult gerbils than middle‐aged gerbils. This study thus suggests an age‐dependent protective effect of ischemic preconditioning against both neuronal apoptosis and dendritic injury in hippocampus after forebrain ischemia.

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“…According to the Paxinos-Watson rat brain stereotaxic coordinates, an incision was made into the skin above the animal’s skull, with a hole then being drilled into the skull. A thin catheter was slowly inserted into the right-cerebral ventricle and fixed with bone cement[20,21]. After surgery, each rat was fed separately and provided with 15000 units of penicillin to avoid infection.…”

Section: Methodsmentioning

confidence: 99%

Study of the effects of nesfatin-1 on gastric function in obese rats

Yang

Zhao

Kong

et al. 2017

WJG

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AIMTo investigate the effects of nesfatin-1 on gastric function in obese rats.METHODSThe obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes.RESULTSBody weight, body length, and Lee’s index of the rats significantly increased in the high-fat diet-induced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. This in vivo experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.CONCLUSIONThrough this research, we provide a theoretical basis for further studies on nesfatin-1, a potential anti-obesity drug.

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In vivo DTI tractography of the rat brain: an atlas of the main tracts in Paxinos space with histological comparison

Cited by 27 publications

References 51 publications

High-resolution three-dimensional macromolecular proton fraction mapping for quantitative neuroanatomical imaging of the rodent brain in ultra-high magnetic fields

High-resolution three-dimensional macromolecular proton fraction mapping for quantitative neuroanatomical imaging of the rodent brain in ultra-high magnetic fields

Protective effects of ischemic preconditioning against neuronal apoptosis and dendritic injury in the hippocampus are age‐dependent

Study of the effects of nesfatin-1 on gastric function in obese rats

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