In vivo confocal Raman spectroscopy and molecular dynamics analysis of penetration of retinyl acetate into stratum corneum

Santos, Laurita dos; S, Claudio A. Téllez; Sousa, Mariane P J; Azóia, Nuno G.; Cavaco-Paulo, Artur; Martin, Aírton Abrahão; Fávero, P. P.

doi:10.1016/j.saa.2016.11.042

Cited by 23 publications

(14 citation statements)

References 44 publications

(57 reference statements)

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“…In addition, it is good for following up the penetration/permeation of APIs [114,115]. Nowadays, Raman microscopy is a powerful technique to properly understand skin structure and percutaneous drug delivery [111,[116][117][118].…”

Section: Confocal Raman Microscopic Methodsmentioning

confidence: 99%

Methods to Evaluate Skin Penetration In Vitro

et al. 2019

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Dermal and transdermal drug therapy is increasing in importance nowadays in drug development. To completely utilize the potential of this administration route, it is necessary to optimize the drug release and skin penetration measurements. This review covers the most well-known and up-to-date methods for evaluating the cutaneous penetration of drugs in vitro as a supporting tool for pharmaceutical research scientists in the early stage of drug development. The aim of this article is to present various experimental models used in dermal/transdermal research and summarize the novel knowledge about the main in vitro methods available to study skin penetration. These techniques are: Diffusion cell, skin-PAMPA, tape stripping, two-photon microscopy, confocal laser scanning microscopy, and confocal Raman microscopic method.

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Section: Confocal Raman Microscopic Methodsmentioning

confidence: 99%

Methods to Evaluate Skin Penetration In Vitro

et al. 2019

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“…Eklouh-Molinier [211] combined CRM and cutometric data into a Partial Least-Squares (PLS) model in an attempt to profile spectral markers associated with ageing, and used this to create a "biological age" chart that mapped skin age based on its molecular state rather than its chronological age. Besides SC thickness and water content, measurements of ceramides/fatty acids and NMF content are possible with CRM, and can be used to assess barrier function [215,216], as well as the penetration of sunscreens [217,218], cosmetic ingredients [219][220][221], and other substances [220,[222][223][224][225][226][227][228].…”

Section: Confocal Raman Microscopymentioning

confidence: 99%

Review of Modern Techniques for the Assessment of Skin Hydration

Qassem

Kyriacou

2019

Cosmetics

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Skin hydration is a complex process that influences the physical and mechanical properties of skin. Various technologies have emerged over the years to assess this parameter, with the current standard being electrical probe-based instruments. Nevertheless, their inability to provide detailed information has prompted the use of sophisticated spectroscopic and imaging methodologies, which are capable of in-depth skin analysis that includes structural and composition details. Modern imaging and spectroscopic techniques have transformed skin research in the dermatological and cosmetics disciplines, and are now commonly employed in conjunction with traditional methods for comprehensive assessment of both healthy and pathological skin. This article reviews current techniques employed in measuring skin hydration, and gives an account on their principle of operation and applications in skin-related research.

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“…Different methods have been described for obtaining depth profiles of topically applied actives using CRS [ 8 , 9 ]. In skin in-vivo studies, in-line measurements have been widely described for monitoring the distribution of actives or excipients on human skin [ 10 , 11 ]. However, the use of human skin faces a lot of difficulties concerning accessibility due to ethnic problems and poor convenience.…”

Section: Introductionmentioning

confidence: 99%

In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy

2021

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Ex-vivo and in-vivo skin analysis has been extensively evaluated by confocal Raman spectroscopy (CRS). The off-line measurement with a CRS-suited skin-mounted device after Franz-cell incubations is the most popular choice. However, real-time monitoring of in-line measurement has clear advantages for obtaining dynamic and more timely results. In our study, a custom-built setup suitable for in-line measurements was implemented, which ensures constant skin incubation and in-situ skin detections. We aim to compare the differences between using in-line and off-line devices for monitoring skin drug penetrations. A well-assessed formulation gel with procaine-HCl as the active ingredient was used as reference. The PEG-23 lauryl ether was added to the formulation as a penetration enhancer to evaluate the enhancement effects of procaine on skin. After incubation times of 14, 20, and 24 h, skin penetration profiles were assessed. Comparable results between off-line and in-line measurements were obtained. Remarkable improvements in penetrated procaine amount and depth were observed. Based on the significant differences of their enhanced penetration amounts, fairly similar estimations were achieved from both methods. A slight difference of 14 h incubation between these two setups can still be found, which may be due to the different detection conditions and affected skin properties. Overall, in-line measurements could provide a more time- and labor-saving alternative for off-line measurements in ex-vivo study.

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In vivo confocal Raman spectroscopy and molecular dynamics analysis of penetration of retinyl acetate into stratum corneum

Cited by 23 publications

References 44 publications

Methods to Evaluate Skin Penetration In Vitro

Methods to Evaluate Skin Penetration In Vitro

Review of Modern Techniques for the Assessment of Skin Hydration

In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy

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