In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry

Abstract

Aims: To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. Methods and Results: Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1 · 10 9 CFU. Lactobacillus johnsonii FI9785 and 24 h later were challenged in separate experiments with S. Enteritidis (S1400, nal r ) and E. coli O78:K80 (EC34195, nal r ). There were no significant effects against S. Enteritidis whereas colonization of the small intestine by E. coli O78:K80 was reduced significantly. Both S. Enteritidis and E. coli colonized the caeca and colon to levels equivalent to control birds and there was no reduction in shedding as assessed by a semi-quantitative cloacal swabbing technique. Specific pathogen-free chicks (20 day old) were dosed with a single oral inoculum of 1 · 10 9 CFU L. johnsonii FI9785 and 24 h later were challenged with C. perfringens. A single oral dose of L. johnsonii FI9785 was sufficient to suppress all aspects of colonization and persistence of C. perfringens. Conclusions: Lactobacillus johnsonii FI9785 may be given to poultry for use as a competitive exclusion agent to control C. perfringens. Significance and Impact of the Study: Lactobacillus johnsonii FI9785 may be a valuable tool to control the endemic disease of necrotic enteritis, thereby reducing economic losses associated with reduced use of antimicrobials in the poultry industry.

The effects of Yeasture® at different rearing stages on intestinal microflora and blood biochemical parameters in male and female broiler chickens

“…In similar case, Murry et al (2006) found lower Coliforms bacteria count in birds by feeding probiotics. Therefore, the beneficial microorganisms establish in the digestive tract and prevent the pathogens (Tellez et al, 2001;La Ragione et al, 2004). Also, in our experiment, Bifidobacterium count was higher at whole period (1 to 42 days).…”

Variations on the Efficacy of Probiotics in Poultry

“…La Ragione & Woodward (2003) verified that the administration of viable spores of Bacillus subtilis to birds free from specific pathogens challenged with C. perfringens reduced the number of pathogens in the spleen, duodenum, colon and cecum, reporting similar results with a probiotic based on Lactobacillus johnsonii (La Ragione et al, 2004). Haghighi et al (2006) shown that a commercial probiotic containing Lactobacillus acidophilus, Bifidobacterium bifidum, and Streptococcus faecalis stimulated the production of antitoxin α IgA from C. perfringens in the intestine of non-vaccinated chicks.…”

Effect of Supplement of Various Antibiotics Alternatives on Performance, Organ Weight, Cecal Microflora, and Blood Characteristics in Broilers

“…또한 Lactobacillus는 Campylobacter (Ghareeb et al, 2012;Neal-McKinney et al, 2012), Clostridium (La Ragione et al, 2004) 및 Salmonella (Chen et al, 2012;Ghareeb et al, 2012) 군집을 감소시켜 가금 식품 안전성을 높인다.…”