In Vivo Characterization of a Decellularized Dermis‐Polymer Complex for Use in Percutaneous Devices

Abstract

To develop a method for making percutaneous devices that have high biocompatibility and do not induce downgrowth of epidermal cells, we prepared a partial decellularized dermis (DD)/poly(methyl methacrylate) (PMMA) complex (PDPC) with a PMMA rod firmly stabilized inside. The porcine decellularized tissue was chosen because of its high biocompatibility and mechanical properties, and MMA was used because it would adhere firmly to a polymer such as a catheter. The MMA filled the cavities in the dermis and polymerized, anchoring to the collagenous fibrils inside the porcine DD. The PDPC was cemented to the PMMA rod tightly and it was integrated with the surrounding tissue within 12 weeks of implantation. Furthermore, no downgrowth of the epidermis, which may cause clinical problems, was observed. We consider that the tissue-polymer complex may be a suitable candidate for use in percutaneous devices.

Decellularized spleen matrix for reengineering functional hepatic-like tissue based on bone marrow mesenchymal stem cells

“…Moreover, the vascular networks preserved in the decellularized scaffold also provide channels for perfusion and implantation in vivo. Studies have demonstrated that decellularized matrix based tissue regeneration is feasible for many hollow and parenchyma organs, such as bladder, 8 skin, 9 blood vessels, 10 heart, 11 lung, 12 kidney, 13 as well as liver. 14 Nonetheless, potential clinical application of DLM-based HLT may be plagued by the selection of scaffold source, considering that human derived DLM is also insufficient, and the application of xenogeneic DLM is hindered by potential risks of zoonosis and immunological rejection.…”

In-situ polymerization of PMMA inside decellularized dermis using UV photopolymerization

“…Since we used a small amount of photoinitiator and we eliminated the monomer inside the complex, cell toxicity was unlikely to be a problem. Furthermore, in our previous work we reported no sign of inflammatory response [4,9]. Therefore, we believe that this complex is safe to use as a base material for tissue interface.…”

“…The decellularized dermis was prepared under high-pressure using the methods described in our previous studies [4,9,[20][21]. In short, in order to dismantle dermis cells, the specimens were sealed inside polyethylene bags containing PBS, and hydrostatically pressurized using a cold isotactic pressurization machine (Dr.…”

“…These methods could have practical applications, for example, in preparation of percutaneous devices, where integration between the artificial catheter and soft tissue is required to prevent the downgrowth of the epidermis [8]. Previously, we showed that monomer absorption and in-situ polymerization can occur using benzyl peroxide (BPO) and N,N′-dimethyl-p-toluidine (DMPT) as initiators, and this method is often used for cementing bones, demonstrating that partial polymer-tissue in-situ polymerization is possible [4,9].…”

Artificial Organs 2014: A Year in Review

“…Kwangwoo Nam et al. of the Tokyo Medical and Dental University, Tokyo, Japan, reported on the development for making percutaneous devices that have high biocompatibility and do not induce downgrowth of epidermal cells. They prepared a porcine partial decellularized dermis (DD)/poly(methyl methacrylate) (PMMA) complex (PDPC) with a PMMA rod firmly stabilized inside.…”