In-situ polymerization of PMMA inside decellularized dermis using UV photopolymerization

Abstract

Polymerization is a technique used to functionalize soft tissues and to produce interface tissues. Here, we developed a method for functionalizing soft tissue with diverse polymers, using in-situ polymerization of monomers that were absorbed into the tissue. Specifically, methyl methacrylate (MMA) and a photoinitiator (Irgacure® 184) were applied to the decellularized dermis, which was polymerized in-situ using irradiating ultraviolet light. MMA polymerization in-situ was possible because the monomers filled the cavities of the decellularized dermis. It took approximately 40 min to complete polymerization, but we found that the rate and depth of polymerization could be adjusted by using mixtures of Irgacure® 184 and Irgacure® 819. The addition of Irgacure® 819 increased polymerization, indicating that polymerization of MMA had occurred inside the dermis. Additionally, we showed that a complex of dermis and poly(methyl methacrylate) (PMMA) increase mechanical strength, which indicates that the PMMA is anchored to collagen fibers. This makes the complex very stable, giving it the functionality of both soft and hard tissue.

Hierarchically Porous Microspheres by Thiol-ene Photopolymerization of High Internal Phase Emulsions-in-Water Colloidal Systems

“…The experiments were carried out using a titanium-based photoinitiator (Irgacure I784) as its absorption peak is in the visible-light spectrum (high absorption of light is between 400 and 480 nm) [ 34 , 35 ]. A custom-made photoreactor was built using blue LED lights with wavelengths between 455 and 460 nm.…”

Overview of the Development, Applications, and Future Perspectives of Decellularized Tissues and Organs

“…Other researchers have attempted to develop novel devices for decellularized tissue using artificial materials. − The development of a device that links native tissues and artificial materials with decellularized tissues, without compatibility issues, is potentially useful. Artificial materials have different mechanical properties from those of native tissues.…”