In vivo blood flow and wall shear stress measurements in the vitelline network

Poelma, Christian; Vennemann, Peter; Lindken, Ralph; Westerweel, J.

doi:10.1007/s00348-008-0476-6

Cited by 81 publications

(98 citation statements)

References 32 publications

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“…The avian embryo is an established model for in vivo studies that investigate the role of flow-induced mechanical loading on vascular remodeling due to the structural and functional similarities between avian and human hearts [13,14]. Most investigations of aortic arch structure and function have been limited to ultrasound and contrast imaging [13], which at the moment, provide insufficient morphology and detailed 3D flow information to quantify the biomechanical events that occur during aortic arch remodeling.…”

Section: Introductionmentioning

confidence: 99%

“…Most investigations of aortic arch structure and function have been limited to ultrasound and contrast imaging [13], which at the moment, provide insufficient morphology and detailed 3D flow information to quantify the biomechanical events that occur during aortic arch remodeling. Several experimental flow visualization techniques including India ink, dye injection [6,11,12,15] and micro particle imaging [14,16,17] have been used to qualitatively and quantitatively document the hemodynamics in chick normal cardiac development. However, these studies focus primarily on 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Wang, Y. et al: Embryonic aortic arch morphogenesis and CFD 4…”

Section: Introductionmentioning

confidence: 99%

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Aortic Arch Morphogenesis and Flow Modeling in the Chick Embryo

et al. 2009

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Morphogenesis of the "immature symmetric embryonic aortic arches" into the "mature and asymmetric aortic arches" involves a delicate sequence of cell and tissue migration, proliferation, and remodeling within an active biomechanical environment. Both patient-derived and experimental animal model data support a significant role for biomechanical forces during arch development. The objective of the present study is to quantify changes in geometry, blood flow, and shear stress patterns (WSS) during a period of normal arch morphogenesis. Composite three dimensional (3D) models of the chick embryo aortic arches were generated at the Hamburger-

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aortic Arch Morphogenesis and Flow Modeling in the Chick Embryo

et al. 2009

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“…Re ta is the cycle averaged Reynolds number: Re ta ¼ Q ta Dr=Am where D and A are the diameter and cross-sectional area of the pipe. Data were processed using a multi-pass PIV algorithm that uses image deformation and correlation averaging [43]. The analysis started with interrogation areas (IAs) of 32 Â 64 pixels and decreased to 4 Â 8 pixels with 50% overlap for the final iterations.…”

Section: Interleaved Ultrasound Imaging Velocimetry Data Acquisition mentioning

confidence: 99%

Ultrasound imaging velocimetry with interleaved images for improved pulsatile arterial flow measurements: a new correction method, experimental and in vivo validation

Fraser

Poelma

Zhou

et al. 2017

J. R. Soc. Interface.

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Blood velocity measurements are important in physiological science and clinical diagnosis. Doppler ultrasound is the most commonly used method but can only measure one velocity component. Ultrasound imaging velocimetry (UIV) is a promising technique capable of measuring two velocity components; however, there is a limit on the maximum velocity that can be measured with conventional hardware which results from the way images are acquired by sweeping the ultrasound beam across the field of view. Interleaved UIV is an extension of UIV in which two image frames are acquired concurrently, allowing the effective interframe separation time to be reduced and therefore increasing the maximum velocity that can be measured. The sweeping of the ultrasound beam across the image results in a systematic error which must be corrected: in this work, we derived and implemented a new velocity correction method which accounts for acceleration of the scatterers. We then, for the first time, assessed the performance of interleaved UIV for measuring pulsatile arterial velocities by measuring flows in phantoms and in vivo and comparing the results with spectral Doppler ultrasound and transit-time flow probe data. The velocity and flow rate in the phantom agreed within 5-10% of peak velocity, and 2-9% of peak flow, respectively, and in vivo the velocity difference was 9% of peak velocity. The maximum velocity measured was 1.8 m s 21 , the highest velocity reported with UIV. This will allow flows in diseased arteries to be investigated and so has the potential to increase diagnostic accuracy and enable new vascular research.

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“…We were therefore obliged to work with the data available. Most work on embryonic blood flow assumes that embryonic blood has the same properties as adult blood (Jones, 2011;Poelma et al, 2008). Thus, the use of data from embryonic blood, even from slightly older stages, represents an advance over previous estimates.…”

Section: Viscosity Estimates In Embryonic Vesselsmentioning

confidence: 99%

Simultaneous imaging of blood flow dynamics and vascular remodelling during development

2015

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Normal vascular development requires blood flow. Time-lapse imaging techniques have revolutionised our understanding of developmental biology, but measuring changes in blood flow dynamics has met with limited success. Ultrasound biomicroscopy and optical coherence tomography can concurrently image vascular structure and blood flow velocity, but these techniques lack the resolution to accurately calculate fluid forces such as shear stress. This is important because hemodynamic forces are biologically active and induce changes in the expression of genes important for vascular development. Regional variations in shear stress, rather than the overall level, control processes such as vessel enlargement and regression during vascular remodelling. We present a technique to concurrently visualise vascular remodelling and blood flow dynamics. We use an avian embryonic model and inject an endothelial-specific dye and fluorescent microspheres. The motion of the microspheres is captured with a high-speed camera and the velocity of the blood flow in and out of the region of interest is quantified by micro-particle image velocitymetry (µPIV). The vessel geometry and flow are used to numerically solve the flow physics with computational fluid dynamics (CFD). Using this technique, we can analyse changes in shear stress, pressure drops and blood flow velocities over a period of 10 to 16 h. We apply this to study the relationship between shear stress and chronic changes in vessel diameter during embryonic development, both in normal development and after TGFβ stimulation. This technique allows us to study the interaction of biomolecular and biomechanical signals during vascular remodelling using an in vivo developmental model.

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In vivo blood flow and wall shear stress measurements in the vitelline network

Cited by 81 publications

References 32 publications

Aortic Arch Morphogenesis and Flow Modeling in the Chick Embryo

Aortic Arch Morphogenesis and Flow Modeling in the Chick Embryo

Ultrasound imaging velocimetry with interleaved images for improved pulsatile arterial flow measurements: a new correction method, experimental and in vivo validation

Simultaneous imaging of blood flow dynamics and vascular remodelling during development

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