In vivo biosynthesis and spatial distribution of Ag nanoparticles in maize ( Zea mays L.)

Abstract

Nanoparticles (NPs), especially biosynthesised in living plants by absorbing soluble salts and reducing metal ions, are extensively used in various fields. This work aimed at investigating the in vivo biosynthesis of silver NPs (Ag-NPs) in maize and the spatial distribution of the NPs and some important nutrient elements in the plant. The content of silver in plant was examined by inductively coupled plasma-atomic emission spectrometer showing that Ag can be absorbed by plant as soluble salts. The NPs in different parts of maize plant were detected and analysed by transmission electron microscopy, demonstrating the synthesis of NPs and their transport from the root to the shoots. Two-dimensional proton induced X-ray emission of silver, chlorine and several nutrient elements elucidated the possible relationship between synthesis of NPs and several nutrient elements in plant tissues. To their knowledge, this is the first report of possibility of synthesis of Ag-NPs in living plants maize ( L.). This study presents direct evidence for synthesis of NPs and distribution of related nutrient elements in maize, which has great significance for studying synthetic application of NPs in crop plants.

Biosynthesis of Ag nanoparticles and two‐dimensional element distribution in Arabidopsis

“…Besides, some other plants have the ability to synthesise metallic NPss [28]. In our research group, we first used living plant maize (Zea mays L.) to synthesise AgNPs [29]. Also, we used Euglena to synthesise AgNPs [30].…”

PSEUDOPEROXIDASE ACTIVITY OF SILVER NANOPARTICLES (AgNPs) AND CHANGES IN PEROXIDASE ACTIVITY IN MAIZE PLANTS EXPOSED TO AgNPs

“…More detailed information on the effect of nanoparticles on plants, including translocation and role in plant metabolism has been summarized by Faraz et al [5]. In several works, AgNPs were prepared with the use of maize extracts [6,7]. After the administration of AgNPs, anatomical changes in maize plants and accumulations of the nanoparticles in their roots were observed [8].…”