In vivo assessment of human skin aging by multiphoton laser scanning tomography

Koehler, Martin; König, Karsten; Elsner, Peter; Bückle, Rainer; Kaatz, Martin

doi:10.1364/ol.31.002879

Cited by 257 publications

(230 citation statements)

References 9 publications

Supporting

Mentioning

212

Contrasting

Unclassified

Order By: Relevance

“…This confirms the fact that the treatment as stronger effect on more aged subjects. In addition, the SAAID level of Group II is always lower than that of the first group, confirming that this parameter decreases with increasing age, as observed in other studies aimed at monitoring skin photo-ageing [54,[58][59][60]. In fact, in Group III SAAID index is increasing after treatment, exhibiting statistically significant variations (after a two sample statistical t-test at the 0.05 level).…”

Section: Saaid Scoring Methodssupporting

confidence: 84%

“…In conclusion, for the first time non-linear microscopy was used in vivo with the final goal of monitoring a laser-based treatment. On the basis of the results described here, and of several other successful dermatological applications experienced in vivo by means of non-linear microscopy in recent years [35][36][37][38][58][59][60], we believe that in the near future non-linear laser scanning microscopy will find a stable place in the clinical dermatological setting.…”

Section: Resultsmentioning

confidence: 76%

See 1 more Smart Citation

In vivo non‐invasive monitoring of collagen remodelling by two‐photon microscopy after micro‐ablative fractional laser resurfacing

Cicchi

Kapsokalyvas²,

Troiano

et al. 2013

Journal of Biophotonics

View full text Add to dashboard Cite

Journal of BIOPHOTONICSNon-linear optical microscopy is becoming popular as a non-invasive in vivo imaging modality in dermatology. In this study, combined TPF and SHG microscopy were used to monitor collagen remodelling in vivo after microablative fractional laser resurfacing. Papillary dermis of living subjects, covering a wide age range, was imaged immediately before and forty days after treatment. A qualitative visual examination of acquired images demonstrated an age-dependent remodelling effect on collagen. Additional quantitative analysis of new collagen production was performed by means of two image analysis methods. A higher increase in SHG to TPF ratio, corresponding to a stronger treatment effectiveness, was found in older subjects, whereas the effect was found to be negligible in young, and minimal in middle age subjects. Analysis of collagen images also showed a dependence of the treatment effectiveness with age but with controversial results. While the diagnostic potential of in vivo multiphoton microscopy has already been demonstrated for skin cancer and other skin diseases, here we first successfully explore its potential use for a non-invasive follow-up of a laser-based treatment.Three-dimensional projection of a stack of 20 SHG images acquired within dermis of a healthy subject. Volume shown: 400 Â 400 Â 100 mm 3 .

show abstract

Section: Saaid Scoring Methodssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 76%

In vivo non‐invasive monitoring of collagen remodelling by two‐photon microscopy after micro‐ablative fractional laser resurfacing

Cicchi

Kapsokalyvas²,

Troiano

et al. 2013

Journal of Biophotonics

View full text Add to dashboard Cite

show abstract

“…This intravital imaging approach also shows its use in the research of skin aging and cancer diagnostics. The ageing skin of young and old people show a significant difference with the semi-quantitative evaluation of dermal collagen and elastin fibre network assessed in vivo by the multiphoton excitation imaging (Koehler et al, 2006). Procedures of selective imaging as well as spectral fluorescence lifetime analysis by means of multiphoton laser tomography support diagnostic decisions and may improve the process of non-invasive early detection of melanoma (König et al, 2007;Dimitrow et al, 2009) (Fig.…”

Section: In Vivo Drug Screening and Administration In Skin And Kidneymentioning

confidence: 99%

Two‐photon microscopy of deep intravital tissues and its merits in clinical research

Wang

König

2010

Journal of Microscopy

174

127

View full text Add to dashboard Cite

SummaryMultiphoton excitation laser scanning microscopy, relying on the simultaneous absorption of two or more photons by a molecule, is one of the most exciting recent developments in biomedical imaging. Thanks to its superior imaging capability of deeper tissue penetration and efficient light detection, this system becomes more and more an inspiring tool for intravital bulk tissue imaging. Two-photon excitation microscopy including 2-photon fluorescence and second harmonic generated signal microscopy is the most common multiphoton microscopic application. In the present review we take diverse ocular tissues as intravital samples to demonstrate the advantages of this approach. Experiments with registration of intracellular 2-photon fluorescence and extracellular collagen second harmonic generated signal microscopy in native ocular tissues are focused. Data show that the in-tandem combination of 2-photon fluorescence and second harmonic generated signal microscopy as two-modality microscopy allows for in situ co-localization imaging of various microstructural components in the whole-mount deep intravital tissues. New applications and recent developments of this high technology in clinical studies such as 2-photon-controlled drug release, in vivo drug screening and administration in skin and kidney, as well as its uses in tumourous tissues such as melanoma and glioma, in diseased lung, brain and heart are additionally reviewed. Intrinsic emission two-modal 2-photon microscopy/tomography, acting as an efficient and sensitive non-injurious imaging approach featured by high contrast and subcellular spatial resolution, has been proved to be a promising tool for intravital deep tissue imaging and clinical studies. Given the level of its performance, we believe Correspondence to B.-G. Wang. Tel: +49 3641 938496; fax: +49 3641 938552; e-mail: Baogui.Wang@mti.uni-jena.de that the non-linear optical imaging technique has tremendous potentials to find more applications in biomedical fundamental and clinical research in the near future.

show abstract

“…The MPT resolution is better than the optical resolution of the microscopes used by pathologists (VIS, NA < 1) to analyze the HE-stained 7 mm thick vertical sections obtained from biopsies (gold standard). Currently, 15 MPT systems are operating in Europe, Asia, and Australia [13] for the early detection of melanoma [14,15] and other skin diseases [16], to track in vivo sunscreen nanoparticles [17] and pharmaceutical compounds as well as to evaluate skin aging [18][19][20][21][22], and the efficiency of anti-aging products [23]. Ultrasonic images cover larger areas and provide information from deeper tissue areas than MPT.…”

Section: Introductionmentioning

confidence: 99%

Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases

König

Speicher²,

Köhler

et al. 2010

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

Journal of BIOPHOTONICSThe first-ever application of high-frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state-of-the-art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two-dimensional color images of the skin surface with a magnification up to 70Â. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide-field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 mm (axial) and 32 mm (lateral). Multiphoton tomography provides 0.36 Â 0.36 Â 0.001 mm 3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 mm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments.Screenshot of human skin of the forearm. For a better visualization the A-scans at different depths are encoded in a 2D B-scan where the brightness represents the amplitudes.

show abstract

In vivo assessment of human skin aging by multiphoton laser scanning tomography

Cited by 257 publications

References 9 publications

In vivo non‐invasive monitoring of collagen remodelling by two‐photon microscopy after micro‐ablative fractional laser resurfacing

In vivo non‐invasive monitoring of collagen remodelling by two‐photon microscopy after micro‐ablative fractional laser resurfacing

Two‐photon microscopy of deep intravital tissues and its merits in clinical research

Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases

Contact Info

Product

Resources

About