<i>In vivo</i> non‐invasive monitoring of collagen remodelling by two‐photon microscopy after micro‐ablative fractional laser resurfacing

Cicchi, Riccardo; Kapsokalyvas, Dimitrios; Troiano, Michela; Campolmi, Piero; Morini, Cristiano; Massi, Daniela; Cannarozzo, Giovanni; Lotti, Torello; Pavone, Francesco S.

doi:10.1002/jbio.201300124

Cited by 40 publications

(44 citation statements)

References 61 publications

(64 reference statements)

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“…The development of high resolution methods of microscopy provided new abilities to study the changes in the spatial organization of the extracellular matrix during the course of inflammation and cancer invasion [10][11][12][13]. The method of nonlinear microscopy in second harmonic generation (SHG) mode allows imaging of collagen fibers in their normal condition and observation of the changes that occur during the process of inflammation and the development of neoplasia [10,14].…”

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confidence: 99%

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Complementary Study of Collagen State in Bladder Diseases Using Cross-Polarization Optical Coherence Tomography, Nonlinear and Atomic Force Microscopy

Kiseleva¹,

Gubarkova²,

Dudenkova³

et al. 2017

Sovrem Tehnol Med

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The aim of the study is to demonstrate the potential of cross-polarization optical coherence tomography (CP OCT) as a minimally invasive real-time technique for detection of bladder cancer against a background of severe inflammation.Materials and Methods. For the verification of CP OCT diagnostic data related to the condition of collagen comparison with highresolution microscopy inspection was performed and correlation of the results was calculated. The CP OCT study was performed on samples of tissue with muscle-invasive urothelial carcinoma T 2-3 N 0-х M 0 (G2, 3) in 18 patients ranging in age from 50 to 64 obtained in course of cystectomy with urine diversion into the intestinal vessel. In total, 60 regions of interest were selected and divided into four groups in accordance with the results of the histological analysis: areas with mild inflammation (group 1, control), 12 areas; areas with severe inflammation (group 2), 18 areas; areas with poorly differentiated urothelial carcinoma with invasion into the muscular layer (group 3), 24 areas; areas with cancer recurrence at the post-operative scar (group 4), 6 areas. Tissue changes at micro-structural level registered by CP OCT were investigated in detail with high-resolution microscopy (nonlinear microscopy and atomic force microscopy). Quantitative processing of all the obtained images enabled their direct comparison.

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confidence: 99%

“…The method of nonlinear microscopy in second harmonic generation (SHG) mode allows imaging of collagen fibers in their normal condition and observation of the changes that occur during the process of inflammation and the development of neoplasia [10,14]. However, this method of microscopy has serious limitations for in vivo endoscopic use [12,15].…”

mentioning

confidence: 99%

Complementary Study of Collagen State in Bladder Diseases Using Cross-Polarization Optical Coherence Tomography, Nonlinear and Atomic Force Microscopy

Kiseleva¹,

Gubarkova²,

Dudenkova³

et al. 2017

Sovrem Tehnol Med

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“…(16) Alongside confocal microscopy, two-photon microscopy is now also being used to detect skin cancer, and some authors have successfully used this method to monitor collagen remodeling in vivo after micro-ablative fractional laser resurfacing. (17) …”

Section: Methodsmentioning

confidence: 99%

Are Incisional and Excisional Skin Tension Lines Biomechanically Different? Understanding the Interplay between Elastin and Collagen during Surgical Procedures

Paul¹

2017

IJBM

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Background: Since Langer's first foray into studying cutaneous lines, although people have studied skin lines across the body, there has not been a study that elucidates changes to skin structure of elastin and collagen at different load levels. This study set out to look at whether incisional lines and excisional lines have different biodynamics and have to be considered differently.Materials and Methods: For this study, we used a two-photon microscopic camera using optimal wavelengths to detect collagen and elastin. Measurements were taken in 5 patients at the center of the excisional wound (high-load) and at the ends of the wound (tapered end of the ellipse) where effectively the wound is an incisional wound.Results: Wounds were observed after they were surgically closed. When incisional wounds were observed, where there was minimal tension (<1.5N force) we found that, in each case, elastin stretched and collagen buckled, revealing mostly elastin. Where larger defects were created after excisions (as in the figure where a skin cancer had been removed, where forces to close wounds were typically greater than 2N), we noted that the image revealed mostly collagen, suggesting that the reverse had occurred (i.e. collagen stretched and elastin buckled). Conclusion:This difference between tension loads on skin and the interplay between collagen and elastin has never before been elucidated for incisional and excisional wounds, and in the author's view has great research interest for a cutaneous surgeon seeking to identify the best skin lines to utilize to minimize scarring.

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“…Two image pattern analysis methods, respectively based on FFT and GLCM, were used to characterize collagen organization in the normal arterial wall and within atherosclerotic plaques [32,33]. Collagen was classified and scored on the basis of its anisotropy, mean fibre size, and mean fibre spacing.…”

Section: Collagen Organization Scored By Image Pattern Analysis Methodsmentioning

confidence: 99%

Non-linear imaging and characterization of atherosclerotic arterial tissue using combined two photon fluorescence, second-harmonic generation and CARS microscopy

et al. 2014

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Atherosclerosis is among the most widespread cardiovascular diseases and one of the leading cause of death in the Western World. Characterization of arterial tissue in atherosclerotic condition is extremely interesting from the diagnostic point of view. Routinely used diagnostic methods, such as histopathological examination, are limited to morphological analysis of the examined tissues, whereas an exhaustive characterization requires a morpho-functional approach. Multimodal non-linear microscopy has the potential to bridge this gap by providing morpho-functional information on the examined tissues in a label-free way. Here we employed multiple non-linear microscopy techniques, including CARS, TPF, and SHG to provide intrinsic optical contrast from various tissue components in both arterial wall and atherosclerotic plaques. CARS and TPF microscopy were used to respectively image lipid depositions within plaques and elastin in the arterial wall. Cholesterol deposition in the lumen and collagen in the arterial wall were selectively imaged by SHG microscopy and distinguished by forward-backward SHG ratio. Image pattern analysis allowed characterizing collagen organization in different tissue regions. Different values of fiber mean size, distribution and anisotropy are calculated for lumen and media prospectively allowing for automated classification of atherosclerotic lesions. The presented method represents a promising diagnostic tool for evaluating atherosclerotic tissue and has the potential to find a stable place in clinical setting as well as to be applied in vivo in the near future.

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In vivo non‐invasive monitoring of collagen remodelling by two‐photon microscopy after micro‐ablative fractional laser resurfacing

Cited by 40 publications

References 61 publications

Complementary Study of Collagen State in Bladder Diseases Using Cross-Polarization Optical Coherence Tomography, Nonlinear and Atomic Force Microscopy

Complementary Study of Collagen State in Bladder Diseases Using Cross-Polarization Optical Coherence Tomography, Nonlinear and Atomic Force Microscopy

Are Incisional and Excisional Skin Tension Lines Biomechanically Different? Understanding the Interplay between Elastin and Collagen during Surgical Procedures

Non-linear imaging and characterization of atherosclerotic arterial tissue using combined two photon fluorescence, second-harmonic generation and CARS microscopy

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