In vivo Assessment of Antidiabetic Potential and Mapping of Pharmacological Properties of Ethanolic Extract of Leaves of Coccinia grandis on Alloxan-induced Diabetic Rats

Islam, Zahirul; Tahsin, Md. Rafat; Faisal, Ahmad Ullah; Tithi, Tanzia Islam; Nova, Tasnova Tasnim; Nila, Tahmina Sharif; Gorapi, Md. Zobayer Hossain; Nadvi, Faisal Ahmed; Mridula, Tasneem Nayla; Choudhury, Abu Asad; Chowdhury, Jakir Ahmed; Kabir, Shaila; Amran, Md. Shah

doi:10.9734/ajarr/2019/v7i230169

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…According to the findings of our study, it was observed that reduction in food intake had no effect on the blood glucose levels of rats. The damaging effects of alloxan-induced cellular atrophy and an initial weight loss in rats treated with alloxan [ 51 ]. However, when the rats (both nondiabetic and diabetic) were treated with test extract ( G. procumbens ), their body weights accelerated just like the rats belonging to other groups.…”

Section: Discussionmentioning

confidence: 99%

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

Tahsin

Tithi

Mim

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Diabetes mellitus is one of the most notable health dilemmas. Analyzing plants for new antidiabetic remedies has become an impressive territory for life science researchers. Gynura procumbens has long been used to treat diabetes. Thus, we strived to ascertain the hypoglycemic potentiality of extract of leaves of G. procumbens by in vivo and in silico approaches. Methods. Fresh leaves of G. procumbens were collected and shade-dried to prepare ethanolic extracts to evaluate pharmacological parameters. Diabetes was induced in rats via injecting alloxan through the intraperitoneal route at a dose of 150 mg/kg body weight. Humalyzer 3000 was used to perform a biochemical assay of collected samples from rats. Anti-hyperglycemic activity study along with overdose toxicity test was performed. The pharmacological activity of this plant was also evaluated through a molecular docking study. This in silico study investigated the binding affinity of natural ligands from G. procumbens against glycoside hydrolase enzymes. Results. We detected a peak plasma concentration of G. procumbens at 3 hours 45 minutes that is roughly similar to the peak plasma concentration of metformin. Again, in OGTT and anti-hyperglycemic tests, it has been ascertained that both plant extract and metformin can exert significant P < 0.05 and highly significant P < 0.01 hypoglycemic activity in a dose-dependent manner. Metformin exhibited better therapeutic efficacy than that of plant extract, but it possessed null statistical significance. Also, our safety profile expressed that, similar to metformin, the plant extract can restore the disturbed pathological state in a dose-oriented approach with a wide safety margin. In silico study also validated the potentialities of natural constituents of G. procumbens. Conclusion. This study suggested that G. procumbens can be considered as potential antidiabetic plant. Robust and meticulous investigation regarding plant chemistry and pharmacology in the future may bring about a new dimension that will aid in discovering antidiabetic drugs from this plant in the diabetes management system.

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Section: Discussionmentioning

confidence: 99%

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

Tahsin

Tithi

Mim

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…The ethanol Coccinia grandis leaves reported a non-significant hypoglycemic effect comparable to the standard drug metformin at 750 mg/kg dose [ 318 ]. Another report by Islam et al 2014 exhibited a substantial reduction in fasting blood glucose levels from C. grandis and Centella asiatica at a dose of 3 mL/kg in both normal and therapeutic models of alloxan-induced diabetic rats [ 319 ].…”

Section: Phytochemical and Experimental Studies Of Antidiabetic Plants In Bangladeshmentioning

confidence: 99%

Ethnomedicinal Value of Antidiabetic Plants in Bangladesh: A Comprehensive Review

Rahman

Uddin

Reza

et al. 2021

Plants

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The use of conventional drugs to treat metabolic disorders and the pathological consequences of diabetes further increases the complications because of the side effects, and is sometimes burdensome due to relatively higher costs and occasionally painful route of administration of these drugs. Therefore, shifting to herbal medicine may be more effective, economical, have fewer side effects and might have minimal toxicity. The present review amasses a list of ethnomedicinal plants of 143 species belonging to 61 families, from distinctive domestic survey literature, reported to have been used to treat diabetes by the ethnic and local people of Bangladesh. Leaves of the medicinal plants were found leading in terms of their use, followed by fruits, whole plants, roots, seeds, bark, stems, flowers, and rhizomes. This review provides starting information leading to the search for and use of indigenous botanical resources to discover bioactive compounds for novel hypoglycemic drug development.

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“…In the 1 st two weeks, the rats were treated with normal food and water without inducing diabetes. Afterward, alloxan was injected into all group 150 mg/kg [19] via intraperitoneal route in all rats those belonged to group 2,3 and 4. Blood glucose levels of these rats were examined to confirm whether they have started suffering from diabetes or not.…”

Section: Groupmentioning

confidence: 99%

An in vivo Evaluation of Antidiabetic and Depressant Action of Bark of Swietenia mahagoni on Alloxan-Induced Diabetic Rat Model

Cruze,

Shanta,

Mostaq

et al. 2020

AJRRE

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Diabetes is a type of medical disorder originated from abnormal metabolic condition and derivatives of natural products are used as a tool to fight against this pernicious disease. Plant is a diversified origin of various chemical compounds of medicinal significance which can be applied to mitigate diabetic disorder. Leafs of Swietenia mahagoni are used for this reason conventionally as a tradition for long period of time. Our purposes were to find out the anti-diabetic effect of Swietenia mahagoni by investigating its extract obtained from its leaves and also establishing its safety profile so that it can be applied to alleviate diabetic disorder. Induction of diabetes in rats were done by applying intraperitoneal injection containing alloxen. Dose of alloxen given were 150 Original Research Articlemg/kg bodyweight. Rats were fed the extract of Swietenia mahagoni leaves by maintaining the dose at 750 mg/kg. Blood glucose level and safety profile were measured by evaluating SGOT,SGPT and creatinine level on both non-diabetic and diabetes induced rats prior and after the extract administration. It was noticed that the anti-diabetic effect was similar to that of metformin (p˃ 0.05) administrated at the dose of 500mg/kg. Investigation of SGPT,SGOT and creatinine level were done to evaluate safety profile. It was observed that the induced diabetic conditions were improved for both metformin and leaf extract. And there were no significant change observed in the rats with nomal physiological condition. Again the extract showed significant depressant effect in tail suspension test and sucrose preference test. It may be therefore surmised that the leaf extract of Swietenia mahagoni could become a potential therapeutic alternative against diabetes mellitus and depressant.

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In vivo Assessment of Antidiabetic Potential and Mapping of Pharmacological Properties of Ethanolic Extract of Leaves of Coccinia grandis on Alloxan-induced Diabetic Rats

Cited by 4 publications

References 7 publications

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

In Vivo and In Silico Assessment of Diabetes Ameliorating Potentiality and Safety Profile of Gynura procumbens Leaves

Ethnomedicinal Value of Antidiabetic Plants in Bangladesh: A Comprehensive Review

An in vivo Evaluation of Antidiabetic and Depressant Action of Bark of Swietenia mahagoni on Alloxan-Induced Diabetic Rat Model

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