In vivo  anticancer activity of maesopsin 4- O - β -glucoside isolated from leaves of  Artocarpus tonkinensis  A. Chev. Ex Gagnep

Thủy, Trịnh Thị; Thiện, Đào Đức; Hung, Tran Quang; Tâm, Nguyễn Thành; Anh, Nguyễn Thị Hoàng; Nga, Nguyễn Thị; Cúc, Nguyễn Thị; Phượng, Lê Thị; Sung, Trần Văn; Delfino, Domenico Vittorio; Thảo, Đỗ Thị

doi:10.1016/j.apjtm.2016.03.012

Cited by 11 publications

(9 citation statements)

References 19 publications

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“…Medicinal properties of leaf and root extracts from A. tonkinensis have long been known and they are recorded in the textbooks of traditional Vietnamese and Chinese medicine. In particular, A. tonkinensis dried leaf decoction and its major active compounds auronol glycosides maesopsin 4-O-β-D-glucoside (TAT-2) and alphitonin-4-O-β-D-glucoside (TAT-6) were studied for their immunosuppressive activity in autoimmune diseases (Thuy et al, 2004;Thuy et al, 2017) and for antiproliferative effect in anti-cancer trials (Thuy et al, 2016). Vietnamese folk medicine practice to assume A. tonkinensis decoction against rheumatoid arthritis (RA) symptoms and several studies on experimental models of collagen-induced arthritis (CIA) (Ngoc et al, 2005;Dang et al, 2009;Adorisio et al, 2019) support the awareness of A. tonkinensis efficacy to contrast chronic inflammation, and destruction of bone and joints characterizing RA pathological picture.…”

Section: Introductionmentioning

confidence: 99%

Artocarpus tonkinensis Extract Inhibits LPS-Triggered Inflammation Markers and Suppresses RANKL-Induced Osteoclastogenesis in RAW264.7

et al. 2021

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Artocarpus tonkinensis (At) leaf decoction, a traditional remedy prepared in North Vietnam by the Hmong ethnic group, is a tea extract rich in bioactive compounds that may have therapeutic effects in arthritis and backache. Indeed, it has been demonstrated that At is able to inhibit Th17 lymphocytes development and to protect mice in an experimental model of collagen-induced arthritis. By resorting to macrophage in vitro models of inflammation and osteoclastogenesis, we showed that At extract significantly reduced nitric oxide synthase 2 (NOS2) activity and IL-6 production by RAW 264.7 murine cells. Moreover, At demonstrated an anti-osteoclastogenic effect, as revealed by complete inhibition of TRAP-positive osteoclast formation and decreased expression of key osteoclast-related genes. This At activity likely relies on the inhibition of RANK downstream signaling pathway, as the activation of non-receptor tyrosine kinase Src is reduced upon RANKL-At exposure. Protective effect of At against bone loss was also enlightened in vivo by collagen-induced arthritis (CIA) experiment demonstrating that, although paw edema was only weakly opposed by drinking At decoction, bone and cartilage were well preserved in CIA+At mice and joint tissue expressed decreased levels of osteoclast marker genes respect to CIA control group. Maesopsin 4-O-β-D-glucoside (i.e., TAT-2, one of the main decoction bioactive components) was capable to contrast NOS2 activity, IL-6 expression and osteoclast formation, too, albeit to a lesser extent when compared to At decoction. Overall, this study enlightens another At cell target, macrophages, beside Th17 lymphocytes, and suggests that the anti-arthritic beneficial effects of At decoction largely derives from its ability to counteract not only inflammation, but also osteoclastogenesis.

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Section: Introductionmentioning

confidence: 99%

Artocarpus tonkinensis Extract Inhibits LPS-Triggered Inflammation Markers and Suppresses RANKL-Induced Osteoclastogenesis in RAW264.7

et al. 2021

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“…Suppression of T cell proliferation and cytokine production by A. tonkinensis flavonoids may reduce disease severity after experimentally induced arthritis (Dang et al, 2009). In anti-cancer trials, maesopsin 4- O -β- D -glucoside (TAT-2) showed antiproliferative effects on acute myeloid leukemia cells and modulated expression of 19 cancer-related genes encoding proteins such as heme oxygenase-1, sulfiredoxin 1 homolog, and breast carcinoma amplified sequence 3 (Pozzesi et al, 2011), and exhibited in vivo anti-cancer effects (Thuy et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Artocarpus tonkinensis Protects Mice Against Collagen-Induced Arthritis and Decreases Th17 Cell Function

et al. 2019

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Artocarpus tonkinensis (Moraceae) is a tree that grows in north Vietnam whose leaf decoction is used as a traditional remedy by the Hmong ethnic group to treat arthritis and backache. Our study evaluated the decoction's efficacy and mechanism of action in DBA/1J mice with collagen-induced arthritis (CIA). Mice treated with the decoction (At) either from the first collagen immunization or after CIA development experienced significantly less joint edema and inflammatory infiltration, whereas CIA-induced cartilage damage could only be prevented by early At treatment. Autoimmune gene expression profiles showed that Th17 cell-associated chemokine CCL20 and cytokines IL-6, IL-17, and IL-22 were strongly downregulated by At. Reduced expression of IL-2, IL-17, IL-22, and FasL in lymph node cells from At-treated mice was further confirmed by real-time PCR. The decoction also inhibited polarization of Th17 cells from CD4 + splenic T cells according to levels of IL-17 and RORC, a Th17 cell-specific transcription factor. Chromatographic analysis identified At's major component as maesopsin-β-D-glucoside, which could inhibit in vitro differentiation of Th17 cells. The decoction significantly alleviated the signs and symptoms of CIA and inhibited the development and function of Th17 cells, highlighting its potent anti-inflammatory activity.

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“…This result was similar to some other studies. In vivo trials of the active ingredient TAT2 extracted from A. tonkinensis leaves have been shown to significantly inhibit tumor growth at 100 and 200 mg/kg body weight (Thuy TT et al, 2016). The AGN ethanol extract was tested against the growth of mouse Lewis Lung Carcinoma (LLC) allograftin syngenic mice or human PC-3 and DU145 prostate cancer xenograft in immunodeficient mice.…”

Section: Discussionmentioning

confidence: 99%

Prodigiosin purification from Serratia marcescens M10 and its antitumor activities

Luong¹,

Thanh²,

Tuyến³

et al. 2021

Vietnam J. Biotechnol.

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Prodigiosin (Pg) is a bright red pigment, which is produced by gram negative and gram positive bacteria including Serratia marcescens, Hahella chejuensis, Vibrio psychroerythrus, Streptomyces coelicolor and many other marine bacteria such as Pseudomonas sp., Vibrio sp. Pg induces apoptosis in different kinds of cancer cells with low toxicity on normal cells. In this study, we purified Pg from solid fermentation of S. marcescens M10 strain and assessed its anticancer activity in tumorized mice. The results showed that Pg was purified by running through a silicagel 60 column with a suitable solvent system of n-hexane:toluene at the rate of 1:1 (v/v) combined with toluene:ethyl acetate at the rate of 9:1 (v/v). TLC plate was used to test the presence of active substances with separation solvent n-hexane:ethyl acetate ratio of 1:1 (v/v). The purity of fractions tested by high performance liquid chromatography method showed as 98%. Purified fractions also showed promising anti-tumor activity in Lewis lung carcinoma induced tumors in BALB/c mice. The tumor volumes in Pg treated groups decreased by 34.18% after 28 days of administration.

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In vivo anticancer activity of maesopsin 4- O - β -glucoside isolated from leaves of Artocarpus tonkinensis A. Chev. Ex Gagnep

Abstract: These results indicate that TAT2 possesses clear antitumor activity. Due to its bioavailability and low toxicity, and the fact that it could be isolated in a large scale from A. tonkinensis leaves, the compound shows promise as a potential anticancer drug.

Cited by 11 publications

References 19 publications

Artocarpus tonkinensis Extract Inhibits LPS-Triggered Inflammation Markers and Suppresses RANKL-Induced Osteoclastogenesis in RAW264.7

Artocarpus tonkinensis Extract Inhibits LPS-Triggered Inflammation Markers and Suppresses RANKL-Induced Osteoclastogenesis in RAW264.7

Artocarpus tonkinensis Protects Mice Against Collagen-Induced Arthritis and Decreases Th17 Cell Function

Prodigiosin purification from Serratia marcescens M10 and its antitumor activities

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