Prodigiosin purification from Serratia marcescens M10 and its antitumor activities

Luong, Vu Trong; Thanh, Nguyen Sy Le; Tuyến, Đỗ Thị; Cuc, Do Thi; Thảo, Đỗ Thị

doi:10.15625/1811-4989/15722

Cited by 4 publications

(4 citation statements)

References 10 publications

(11 reference statements)

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“…Prodigiosin was produced by Serratia marcescens M10 (QBN VTCC 910027) and purified basically as described previously [ 19 ] using a dual-step silica-gel column chromatography protocol (first-step solvent of toluene/ethyl acetate = 9:1 ( v / v ) and second-step solvent of toluene/ethyl acetate = 7:3 ( v / v )). The stock solution of prodigiosin (0.1 mg/mL dimethyl sulfoxide (DMSO)) was stored at −20 °C under light-shielded conditions.…”

Section: Methodsmentioning

confidence: 99%

Induction of Endoplasmic Reticulum Stress by Prodigiosin in Yeast Saccharomyces cerevisiae

Nguyen,

et al. 2024

CIMB

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Prodigiosin, a red pigment produced by numerous bacterial species, exerts various antibiotic effects on prokaryotic and eukaryotic organisms. For instance, human carcinoma cell lines appear to suffer from endoplasmic reticulum (ER) stress in the presence of prodigiosin. Here, we demonstrated that prodigiosin also triggers the unfolded-protein response (UPR), which is a cytoprotective response against ER stress, in yeast Saccharomyces cerevisiae. An S. cerevisiae mutant carrying a UPR-deficient mutation was hypersensitive to prodigiosin. Our observations cumulatively indicate that protein folding in the ER is impaired by prodigiosin, illustrating a new mode of action.

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Section: Methodsmentioning

confidence: 99%

Induction of Endoplasmic Reticulum Stress by Prodigiosin in Yeast Saccharomyces cerevisiae

Nguyen,

et al. 2024

CIMB

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“…and identification of prodigiosin from S. marcescens S. marcescens QBN M5 strain will be cultured on NA medium to biosynthesize Pg. The determination of an effective solvent system for Pg extraction from S. marcescens culture was previously established (Vu et al, 2021). In our present investigation, we employed a solvent mixture of ethyl acetate ** and acetone (1:1) for extracting prodigiosin from S. marcescens.…”

Section: Purificationmentioning

confidence: 99%

Mutation of rsmC gene from Serratia marcescens QBN using CRISPR-Cas9 to enhance prodigiosin synthesis

Thanh,

Thi Thao,

Tuyen

et al. 2023

Vietnam J. Biotechnol.

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Prodigiosin is a bright red pigment, which is produced by various gram-negative and gram positive bacteria include S. marcescens, Hahella chejuensis, Vibrio psychroerythrus, Streptomyces coelicolor and many marine bacteria such as Pseudomonas sp, Vibrio sp. Prodigiosin has garnered considerable interest among the scientific community due to its immunosuppressive and anticancer properties against various drug-resistant cancer cell lines. In this study, we utilized the biosynthetic pathway of prodigiosin (Pg), using CRISPR/Cas9 gene editing techniques to eliminate the gene inhibiting Pg synthesis, known as rsmC, aiming to enhance the ability to synthesize Pg from S. marcescens strains. The rsmC gene of S. marcescens QBN was randomly mutated using the CRISPR-Cas9 system with the pCasPA vector (containing Cas9) and pCRISPR (carrying the spacer segment and sgRNA synthesis). A 20-nucleotide spacer segment was selected on rsmCpreceding the TGG triplet at the 3' end (PAM, the recognition site of Cas9) and inserted into pCRISPR between two BsaI sites. Following the binding of sgRNA to Cas9, mutations occurred in the nucleotides of the rsmC gene, resulting in the cleavage of the double-stranded DNA at the targeted site. A sequence homologous to the rsmC gene, lacking 100 nucleotides, was introduced into the cell and will involve the cleaved DNA's repair mechanism. The mutated rsmC strain of S. marcescens QBN synthesized Pg at 216 - 231 mg/L, higher than the original strain (180 mg/L). Furthermore, HPLC results and 1H spectral analysis showed that the synthesized red pigment from the mutated strain S. marcescens QBN was prodigiosin. These findings provide a basis for further improving the productivity of Pg synthesis in the S. marcescens QBN strain and serve as a basis for editing genes in other microorganisms using the CRISPR-Cas9 system.

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“…PG could be easily purified by running through a silica gel 60 columns with a suitable solvent system of n-hexane: toluene at the rate of 1:1 (v/v) combined with toluene: ethyl acetate at the rate of 9:1 (v/v) and gave 98% purity. A similar procedure was obtained in 92% purity [ 67 , 68 ].…”

Section: Prodigiosin Purificationmentioning

confidence: 99%

“…PG showed anti-tumor activity in vivo in Lewis lung carcinoma-induced tumors in BALB/c mice. The tumor volumes in PG treated groups decreased by 34.18% after 28 days of administration [ 67 ].…”

Section: Biological Activities Of Prodigiosinmentioning

confidence: 99%

Prodigiosin: a promising biomolecule with many potential biomedical applications

et al. 2022

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Pigments are among the most fascinating molecules found in nature and used by human civilizations since the prehistoric ages. Although most of the bio-dyes reported in the literature were discovered around the eighties, the necessity to explore novel compounds for new biological applications has made them resurface as potential alternatives. Prodigiosin (PG) is an alkaloid red bio-dye produced by diverse microorganisms and composed of a linear tripyrrole chemical structure. PG emerges as a really interesting tool since it shows a wide spectrum of biological activities, such as antibacterial, antifungal, algicidal, anti-Chagas, anti-amoebic, antimalarial, anticancer, antiparasitic, antiviral, and/or immunosuppressive. However, PG vehiculation into different delivery systems has been proposed since possesses low bioavailability because of its high hydrophobic character (XLogP3-AA = 4.5). In the present review, the general aspects of the PG correlated with synthesis, production process, and biological activities are reported. Besides, some of the most relevant PG delivery systems described in the literature, as well as novel unexplored applications to potentiate its biological activity in biomedical applications, are proposed.

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Prodigiosin purification from Serratia marcescens M10 and its antitumor activities

Cited by 4 publications

References 10 publications

Induction of Endoplasmic Reticulum Stress by Prodigiosin in Yeast Saccharomyces cerevisiae

Induction of Endoplasmic Reticulum Stress by Prodigiosin in Yeast Saccharomyces cerevisiae

Mutation of rsmC gene from Serratia marcescens QBN using CRISPR-Cas9 to enhance prodigiosin synthesis

Prodigiosin: a promising biomolecule with many potential biomedical applications

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