In vivo and in vitro degradation of poly(3-hydroxybutyrate) in pat

Saito, Terumi; Tomita, Kazuyoshi; Juni, Kazuhiko; Ooba, Kenkichi

doi:10.1016/0142-9612(91)90039-d

Cited by 80 publications

(37 citation statements)

References 11 publications

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“…Natural polymers were also explored, e. g., collagen27) and poly(3-hydroxybutyric acid) (PHB) or poly[(3-hydroxybutyric acid)-co-(3-hydroxyvaleric acid)] (PHBIHV) [28][29][30][31][32][33][34][35][36][37][38]. Summarizing the reslts of these investigations, one is led to the conclusion that the plurality of demands on the implants cannot be met by the materials known to date39).…”

Section: Tab 1 Nentlymentioning

confidence: 99%

Synthesis of degradable, biocompatible, and tough block‐copolyesterurethanes

Hirt¹,

Neuenschwander²,

Suter³

1996

Macro Chemistry & Physics

117

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We describe the synthesis of micro-phase-segregated block-copolyesterurethanes from telechelic hydroxyterminated poly [ [(R)-3-hydroxybutyric acid]-co-[(R)-3-hydroxyvaleric acid]] (PHB-diol) as "hard segments" (i. e., crystallizable chain sections) and hydroxyterminated poly(ecapro1actone)-diethylene glycol-poly(8-caprolactone) (PCL-diol) or telechelic .hydroxyterminated pol y [(adipic acid)-alt-( 1,4-butanediol; diethylene glycol; ethylene glycol)] (Diorez") as "soft segments", with 2,2,4-triethylhexamethylene diisocyanate (TMDI) or methyl (S)-2,6-diisocyanatohexanoate (LDI). High molecular weights were obtained with or without catalyst, the properties of the polymers depending only slightly on the presence or absence of the catalyst. The materials thus obtained were investigated also with respect to their mechanical properties and it was found that Young's modulus directly depends on the fraction of crystallizable PHB-diol in the block copolymer while the type of non-crystallizable segment or diisocyanate had only a minor influence: Generally, the tensile strength increases and the elongation at break decreases with increasing content of PHB-diol. Around body temperature, these polymers exhibit only mild changes in their mechanical behavior. The chain length of the non-crystallizable segment indirectly influences the morphology and the mechanical properties of the polymers through changes in the phase-segregation behavior.

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Section: Tab 1 Nentlymentioning

confidence: 99%

Synthesis of degradable, biocompatible, and tough block‐copolyesterurethanes

Hirt¹,

Neuenschwander²,

Suter³

1996

Macro Chemistry & Physics

117

View full text Add to dashboard Cite

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“…Recently, there has been increasing interest in constructing desirable extracellular microenvironments on the surface of biomaterials in order to obtain optimal cell and/or tissue responses. Studies with polyhydroxybutyrate (PHB) membranes and mesenchymal stem cells (MSCs), which have high differentiation capacity, showed that PHB membranes induced MSCs to osteogenic differentiation in vitro and in vivo (Wang et al, 2004;Wollenweber et al, 2006;Rentsch et al, 2010) PHB is the most thoroughly investigated member of the PHA family and has shown good biocompatibility with different cell types, including mouse fibroblast cell lines (Yang et al, 2002), chondrocytes (Saito et al, 1991), osteoblasts (Köse et al, 2003a(Köse et al, , 2003b, endothelial cells (Shishatskaya and Volova, 2004), and gastrointestinal cells in rats (Freier et al, 2002). Although PHB is inherently biodegradable and biocompatible, the use of PHB is significantly limited in biomedical applications due to several characteristics, such as brittleness, rigidity, and low mechanical properties (Engelberg and Kohn, 1991;Misra et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of biocompatibility of random or aligned electrospun polyhydroxybutyrate scaffolds combined with human mesenchymal stem cells

Köse¹,

Aerts‐Kaya²,

Denkbaş³

et al. 2016

Turk J Biol

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Polyhydroxybutyrate (PHB) is a polymer used to restore tissues or regenerate bones. However, its compatibility with human bone marrow-derived mesenchymal stem cells (MSCs) has not been examined. PHB membranes of random (r-PHB) or aligned (a-PHB) electrospun nanofibers that generate bone and spinal axon scaffolds were combined with human MSCs. The adhesion and proliferation of cells on these membranes were examined. The orientation of cells on PHB membranes was analyzed by confocal microscopy and scanning electron microscopy (SEM). The MSCs maintained their characteristic properties on the membranes, adhered to the membranes, and preserved their viability. The cell morphology was different when they were grown on differentially designed matrices. Cells expanded on the a-PHB membrane and showed fibroid-like morphology. Conversely, cells interacting with the r-PHB membrane were located homogeneously and demonstrated polygonal morphology. The adhesion and proliferation of human MSCs was higher on the a-PHB membrane than on the randomly oriented one. Fiber orientation influenced the phenotype and biological behavior of human MSCs. This property may be useful in the selection of specifically designed scaffolds for the desired tasks. The results of this preliminary study indicated that PHB membranes designed for bone or nerve tissue engineering are compatible with human MSCs.

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“…Several in vivo studies have shown that PHB may serve as a scaffold for tissue engineering due to its excellent biocompatibility as evidenced by lack of toxicity and compatibility with tissue and blood (Saito et al 1991;Clarotti et al 1992;Gogolewski et al 1993;Schmack et al 2000;Mai et al 2006;Mack et al 2008;Gredes et al 2009). PHB sheets did not caused any inflammation in the chorioallantoic membrane of the developing egg (Saito et al 1991). In mouse tissues no acute inflammation, abscess formation, or tissue necrosis was observed after subcutaneously implantation.…”

Section: Poly-3-hydroxybutyrate (Phb)mentioning

confidence: 99%