In Vivo and In Vitro Models of Psoriasis

Jean, Jessica; Pouliot, Roxane

doi:10.5772/8582

Cited by 15 publications

(29 citation statements)

References 92 publications

(155 reference statements)

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“…Higher keratinocyte proliferation values were obtained in the presence of psoriatic fibroblasts (Saiag et al, 1985). Furthermore, this model led to the conclusion that normal fibroblasts are unable to suppress the hyperproliferative growth of psoriatic keratinocytes, and that hyperproliferation of normal epidermis can be induced both by uninvolved and involved psoriatic fibroblasts (Saiag et al, 1985, Jean & Pouliot, 2010.…”

Section: Organ Culturementioning

confidence: 90%

“…Thus, the amount of transplant rejection is reduced in the AGR129 model compared to the others. Boyman et al demonstrated that human uninvolved psoriatic skin grafted onto AGR129 mice spontaneously developed psoriatic plaques without the injection of any activated immune cells or any other exogenous factor, suggesting that uninvolved psoriatic skin is not exactly comparable to the normal human skin of healthy patients (Boyman et al, 2004, Gudjonsson et al, 2007, Jean & Pouliot, 2010. However, the absence of an inflammatory system could be a significant weakness of these models, since the importance of the immunology has been described by many research groups.…”

Section: Infiltration Of Inflammatory Cells In the Skinmentioning

confidence: 99%

“…These models allow the isolation of one cell type for step by step dissection of the implied mechanisms. Even if it was not possible to observe direct interaction between cell types, these models allowed the discovery of many interesting facts about psoriasis, and favoured a better understanding of the pathology (Jean & Pouliot, 2010).…”

Section: Monolayermentioning

confidence: 99%

“…There exist a broad variety of genetically modified in vivo models. An exhaustive list can be seen in table 2 (Jean & Pouliot, 2010 Jean et al, 2010 according to the copyright policy of the publisher.  2010 InTech.…”

Section: Genetically Modified Modelsmentioning

confidence: 99%

“…Animal models based on transgenic technology have been used extensively to study the pathogenesis of various skin diseases, including psoriasis (Raychaudhuri et al, 2001, Jean & Pouliot, 2010. Xenotransplantation approach consists of grafting a piece of in vivo psoriatic skin (or an in vitro psoriatic substitute) on a genetically modified mouse.…”

Section: Xenotransplantationmentioning

confidence: 99%

See 4 more Smart Citations

Psoriatic Skin Models: A Need for the Pharmaceutical Industry

Jean¹,

García-Pérez²,

Pouliot³

2012

Psoriasis

Self Cite

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Section: Organ Culturementioning

confidence: 90%

Section: Infiltration Of Inflammatory Cells In the Skinmentioning

confidence: 99%

Section: Monolayermentioning

confidence: 99%

Section: Genetically Modified Modelsmentioning

confidence: 99%

Section: Xenotransplantationmentioning

confidence: 99%

See 3 more Smart Citations

Psoriatic Skin Models: A Need for the Pharmaceutical Industry

Jean¹,

García-Pérez²,

Pouliot³

2012

Psoriasis

Self Cite

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Simulation of organ-on-a-chip systems

Filipović

Nikolić

Šušteršič

2020

Biomaterials for Organ and Tissue Regeneration

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A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions

Duque-Fernandez

Gauthier

Simard

et al. 2016

Biochemistry and Biophysics Reports

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BackgroundInadequate representation of the human tissue environment during a preclinical screen can result in inaccurate predictions of compound effects. Consequently, pharmaceutical investigators are searching for preclinical models that closely resemble original tissue for predicting clinical outcomes.MethodsThe current research aims to compare the impact of using serum-free medium instead of complete culture medium during the last step of psoriatic skin substitute reconstruction. Skin substitutes were produced according to the self-assembly approach.ResultsSerum-free conditions have no negative impact on the reconstruction of healthy or psoriatic skin substitutes presented in this study regarding their macroscopic or histological appearances. ATR-FTIR results showed no significant differences in the CH2 bands between psoriatic substitutes cultured with or without serum, thus suggesting that serum deprivation did not have a negative impact on the lipid organization of their stratum corneum. Serum deprivation could even lead to a better organization of healthy skin substitute lipids. Percutaneous analyses demonstrated that psoriatic substitutes cultured in serum-free conditions showed a higher permeability to hydrocortisone compared to controls, while no significant differences in benzoic acid and caffeine penetration profiles were observed.ConclusionsResults obtained with this 3D-psoriatic skin substitute demonstrate the potential and versatility of the model. It could offer good prediction of drug related toxicities at preclinical stages performed in order to avoid unexpected and costly findings in the clinic.General significanceTogether, these findings offer a new approach for one of the most important challenges of the 21st century, namely, prediction of drug toxicity.

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In Vivo and In Vitro Models of Psoriasis

Cited by 15 publications

References 92 publications

Psoriatic Skin Models: A Need for the Pharmaceutical Industry

Psoriatic Skin Models: A Need for the Pharmaceutical Industry

Simulation of organ-on-a-chip systems

A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions

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