Simulation of organ-on-a-chip systems

Filipović, Nenad; Nikolić, Milena; Šušteršič, Tijana

doi:10.1016/b978-0-08-102906-0.00028-3

Cited by 10 publications

(3 citation statements)

References 55 publications

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“…Figure S1 shows visualized modeling to simulate the pressure (A) and flow velocity (B) and distribution of shear stress (C) applied inside the sensor chip using COMSOL Multiphysics ® software version 5.4. The pressure distribution values of the simulation presented coincided with the shear stress simulation modeling of the chip [7,38]. The standard of the ROS sensor chip was also confirmed and implemented in a COMSOL environment.…”

Section: Biomimetic Microfluidic System Calibrationsupporting

confidence: 66%

Cellular Efficacy of Fattigated Nanoparticles and Real-Time ROS Occurrence Using Microfluidic Hepatocarcinoma Chip System: Effect of Anticancer Drug Solubility and Shear Stress

Kim,

Ngo

et al. 2023

Pharmaceuticals

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The objective of this study was to evaluate the effectiveness of organ-on-chip system investigating simultaneous cellular efficacy and real-time reactive oxygen species (ROS) occurrence of anticancer drug-loaded nanoparticles (NPs) using hepatocarcinoma cells (HepG2) chip system under static and hepatomimicking shear stress conditions (5 dyne/cm2). Then, the role of hepatomimetic shear stress exposed to HepG2 and drug solubility were compared. The highly soluble doxorubicin (DOX) and poorly soluble paclitaxel (PTX) were chosen. Fattigated NPs (AONs) were formed via self-assembly of amphiphilic albumin (HSA)-oleic acid conjugate (AOC). Then, drug-loaded AONs (DOX-AON or PTX-AON) were exposed to a serum-free HepG2 medium at 37 °C and 5% carbon dioxide for 24 h using a real-time ROS sensor chip-based microfluidic system. The cellular efficacy and simultaneous ROS occurrence of free drugs and drug-loaded AONs were compared. The cellular efficacy of drug-loaded AONs varied in a dose-dependent manner and were consistently correlated with real-time of ROS occurrence. Drug-loaded AONs increased the intracellular fluorescence intensity and decreased the cellular efficacy compared to free drugs under dynamic conditions. The half-maximal inhibitory concentration (IC50) values of free DOX (13.4 μg/mL) and PTX (54.44 μg/mL) under static conditions decreased to 11.79 and 38.43 μg/mL, respectively, under dynamic conditions. Furthermore, DOX- and PTX-AONs showed highly decreased IC50 values of 5.613 and 21.86 μg/mL, respectively, as compared to free drugs under dynamic conditions. It was evident that cellular efficacy and real-time ROS occurrence were well-correlated and highly dependent on the drug-loaded nanostructure, drug solubility and physiological shear stress.

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Section: Biomimetic Microfluidic System Calibrationsupporting

confidence: 66%

Cellular Efficacy of Fattigated Nanoparticles and Real-Time ROS Occurrence Using Microfluidic Hepatocarcinoma Chip System: Effect of Anticancer Drug Solubility and Shear Stress

Kim,

Ngo

et al. 2023

Pharmaceuticals

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“…OoC devices emerged to fill the gap between animal testing and two-dimensional in vitro models which are inadequate to mimic the physiology of the human organs [3,4]. Besides, OoCs have greatly contributed to the 3Rs principle, Refine, Reduce, and Replace [5]. By combining microfluidic technology with cell culture, these devices have been applied to study alternative therapies for numerous diseases, such as cancer [6].…”

Section: Introductionmentioning

confidence: 99%

Influence of boundary conditions on oxygen distribution in an organ-on-a-chip platform

Carvalho¹,

Rodrigues²,

Rodrigues³

et al. 2022

8th European Congress on Computational Methods in Applied Sciences and Engineering

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Organ-on-a-chip (OoC) platforms have revolutionized the drug development process by offering an effective alternative to animal models. These advanced microfluidic platforms mimic the organ functions at a microscale, and they can be produced at a large scale and at a lower price. Despite the variety of OoC models developed up to now, the combination of numerical simulations with experimental procedures has been of paramount importance in the development of more realistic and effective OoC devices. In addition, a better understanding of the physical phenomena happening in OoC can be obtained. In the present work, fluid flow numerical simulations were carried out in an OoC aiming to evaluate the influence of imposing different inlet velocities on the oxygen distribution along the device. This is of great importance to understand if the oxygen that reaches the cells is adequate for their culture. The results showed that for the geometry tested, with four organ models in parallel, by increasing the inlet velocity, the dissolved oxygen where cells are cultured also increases. This proves the importance of using numerical simulations for improving the performance of the experimental tests and optimizing the flow conditions.

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“…Over the years, the demand for more reliable and effective preclinical models has grown as we try to reduce and, where possible, replace the utilization of animal models for assessing drug efficacy and safety [1]. Although animal models provide a physiological structure similar to that of human cells and organs, they fail to adequately forecast the behavior of and represent human metabolism.…”

Section: Introductionmentioning

confidence: 99%

Computational Simulations in Advanced Microfluidic Devices: A Review

et al. 2021

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Numerical simulations have revolutionized research in several engineering areas by contributing to the understanding and improvement of several processes, being biomedical engineering one of them. Due to their potential, computational tools have gained visibility and have been increasingly used by several research groups as a supporting tool for the development of preclinical platforms as they allow studying, in a more detailed and faster way, phenomena that are difficult to study experimentally due to the complexity of biological processes present in these models—namely, heat transfer, shear stresses, diffusion processes, velocity fields, etc. There are several contributions already in the literature, and significant advances have been made in this field of research. This review provides the most recent progress in numerical studies on advanced microfluidic devices, such as organ-on-a-chip (OoC) devices, and how these studies can be helpful in enhancing our insight into the physical processes involved and in developing more effective OoC platforms. In general, it has been noticed that in some cases, the numerical studies performed have limitations that need to be improved, and in the majority of the studies, it is extremely difficult to replicate the data due to the lack of detail around the simulations carried out.

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Simulation of organ-on-a-chip systems

Cited by 10 publications

References 55 publications

Cellular Efficacy of Fattigated Nanoparticles and Real-Time ROS Occurrence Using Microfluidic Hepatocarcinoma Chip System: Effect of Anticancer Drug Solubility and Shear Stress

Cellular Efficacy of Fattigated Nanoparticles and Real-Time ROS Occurrence Using Microfluidic Hepatocarcinoma Chip System: Effect of Anticancer Drug Solubility and Shear Stress

Influence of boundary conditions on oxygen distribution in an organ-on-a-chip platform

Computational Simulations in Advanced Microfluidic Devices: A Review

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