In vivo and in vitro metabolism of estrone and estradiol-17β and their 3-sulfates in pregnant female guinea-pigs: A plausible prehormone role of estrogen-sulfates in the maternal uterus

Adessi, G. L.; Nhuan, Tran Quang; Vingler, Philippe

doi:10.1016/0022-4731(82)90151-0

Cited by 32 publications

(19 citation statements)

References 25 publications

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“…It is known that milk is a complex matrix containing a lot of possible interferents that can act at both extraction and detection level; therefore, low recovery and/or strong matrix effect are the main problems arising when a simple SPE was tested to determine free and conjugated metabolites in milk [10,20]. For this reason, usually the authors propose the deproteinization of milk as the first step in sample preparation methods, and then a fraction of the sample is cleaned up and analyzed, while a second fraction is deconjugated, cleaned up by LLE or SPE [5], and then analyzed for total estrogens.…”

Section: Optimization Of Extraction and Cleanup Proceduresmentioning

confidence: 99%

“…Conjugated estrogens are usually determined as total free steroids after enzymatic deconjugation by β-glucuronidase and aryl sulfatase of the conjugated forms [19,20]. The analysis is then performed twice in order to calculate the estrogen freed from metabolites by subtracting the free estrogen concentrations (before enzymatic cleavage) from the total estrogens (after enzymatic cleavage).…”

Section: Introductionmentioning

confidence: 99%

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Ultra-high-performance liquid chromatography-tandem mass spectrometry for the analysis of free and conjugated natural estrogens in cow milk without deconjugation

et al. 2014

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A sensitive liquid chromatography/electrospray ionization-tandem mass spectrometry method for the determination of free and conjugated estrogens in cow milk is described. Milk samples were diluted with water and then extracted and cleaned up by solid-phase extraction using graphitized carbon black as adsorbent material, without any enzymatic cleavage, derivatization, and/or protein precipitation step. Two fractions were collected (free and conjugated estrogens) and analyzed separately. Mass spectrometry analysis was performed in negative ion mode using selected reaction monitoring acquisition mode. For all compounds, the coefficients of determination (R(2)) ranged between 0.9892 and 0.9997. Analytical recoveries were in the range of 86-109% for free estrogens and 85-118% for conjugates, with relative standard deviations below 10%, and the method detection limit ranged between 3 and 80 ng L(-1). Finally, the developed method was used to determine the presence of free and conjugated estrogens in six retail milk samples (five cow milk samples and one goat milk sample) and one goat milk sample provided by a local shepherd. Estrone was found to be the major free estrogen present in commercial milk samples, and estrone 3-sulfate showed the highest concentration among the target conjugated estrogens. Estriol was also observed in some analyzed milk samples, but the concentrations were always below the limit of quantification.

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Section: Optimization Of Extraction and Cleanup Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultra-high-performance liquid chromatography-tandem mass spectrometry for the analysis of free and conjugated natural estrogens in cow milk without deconjugation

et al. 2014

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“…Laure & Pasqualini (1983) have demonstrated that oestradiol-17ß increased the RNA polymerase in fetal uterus. Moutaouakkil, Prost, Dahan & Adessi (1984) have studied sulphatase activities in the uterus, and a metabolic pathway of oestrogens in pregnant female guinea-pigs has been proposed (Adessi, Tran Quang Nhuan & Prost, 1981;Adessi, Tran Quang Nhuan & Vingler, 1982).…”

Section: Introductionmentioning

confidence: 99%

“…The method has been described previously by Adessi et al (1982). The mass-spectrometer used was a RIBER R 10-10 (Ribermag, Rueil, France) coupled with a gas-chromatograph GIRDEL 30 (Girdel, Puteaux, France) equipped with a SE-30 0-2 mm open tubular fused-silica capillary column.…”

Section: Introductionmentioning

confidence: 99%

Culture of epithelial and stromal cells of guinea-pig endometrium and the effect of oestradiol-17 on the epithelial cells

Chaminadas¹,

Propper²,

Royez³

et al. 1986

Reproduction

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“…STS desulfates a number of 3-β-hydroxysteroid sulfates, converting inactive steroid hormone to the active form [1]. X-linked ichthyosis (XLI) is an inherited skin disorder caused by a deficiency of STS enzyme activity [2].…”

Section: Introductionmentioning

confidence: 99%

Molecular Analysis of X-Linked Ichthyosis in Japan

Sugawara¹,

Fujimoto

2001

Horm Res Paediatr

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Background: X-linked ichthyosis (XLI) is an inherited skin disorder caused by a deficiency of steroid sulfatase (STS). The gene and protein of STS were examined in 19 Japanese patients with XLI. Results: In Western blotting analysis, no cross-reacting peptide was detected in the patients’ placenta, although a single band (63 kD) corresponding to STS in a normal subject was observed. Southern blotting was performed using EcoRI digests of cellular DNA from 13 XLI patients and full-length human STS cDNA as a probe. Normal males had bands of 20, 15, 10, 9.0, 6.1, 4.2, 2.6, and 1.5 kb. Twelve of the 19 patients had only 20- and 1.5-kb bands. Only one patient had the same band pattern as that of normal males. The STS gene was analyzed by PCR in 6 of the 19 patients. PCR amplification products were sequenced to analyze the STS gene. Two cases with one-base change in the STS gene and variation in amino acids H444R and E560P were found. Mutant STS cDNA was transfected into COS-1 cells and the STS enzyme activity was assayed. The enzyme activities were less than the minimum detection value of the detection system. Conclusions: These results suggest that XLI is mainly caused by an extensive deletion of the STS gene and that the PCR method is useful for detection of STS point mutations.

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In vivo and in vitro metabolism of estrone and estradiol-17β and their 3-sulfates in pregnant female guinea-pigs: A plausible prehormone role of estrogen-sulfates in the maternal uterus

Cited by 32 publications

References 25 publications

Ultra-high-performance liquid chromatography-tandem mass spectrometry for the analysis of free and conjugated natural estrogens in cow milk without deconjugation

Ultra-high-performance liquid chromatography-tandem mass spectrometry for the analysis of free and conjugated natural estrogens in cow milk without deconjugation

Culture of epithelial and stromal cells of guinea-pig endometrium and the effect of oestradiol-17 on the epithelial cells

Molecular Analysis of X-Linked Ichthyosis in Japan

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