In Vivo and in Vitro Studies of the Effect of Chitosan on the Bone Defect Repair Process

Зудина, И. В.; Vedyaeva, Anna; Ivanov, Pavel; Alzubaidi, A. F. A.; Булкина, Н. В.

doi:10.18500/1816-9775-2016-16-2-171-179

Cited by 4 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…При этом создаются оптимальные условия заживления раны во влажной среде и ее защита от вторичной контаминации за счет пролонгированного выделения в раневую среду частиц коллоидного серебра [3,9,10]. Описано, что хитозан стимулирует пролиферацию клеток и гистоархитектурную организацию тканей, стимулирует эпителиальные клетки и фибробласты, способствует синтезу фактора роста эндотелия сосудов (VEGF) и гранулоцитарномакрофагального колониестимулирующего фактора (GM-CSF), сохраняя уровень активности естественных факторов роста [6,12], а коллаген уменьшает активность протеолитических ферментов в ране и, таким образом, способствует формированию грануляционной ткани [2]. Данное обстоятельство нашло подтверждение и в нашем исследовании при описании гистопрепаратов кожи и анализе морфометрических данных.…”

unclassified

Влияние Хитозан-Коллагенового Комплекса С Наночастицами Серебра На Течение Раневого Процесса В Эксперименте

Бежин¹,

Липатов²,

Фрончек³

et al. 2019

Курск. науч.-практ. вестн. «Человек и его здоровье»

View full text Add to dashboard Cite

¹ Курский государственный медицинский университет (КГМУ) Россия, 305041, Курская область, г. Курск, ул. К. Маркса, д. 3 2 ООО «Эверс Груп Рус» Россия, 125493, г. Москва, ул. Флотская, 5 Цель: изучить в эксперименте локальное воздействие хитозан-коллагенового комплекса с наночастицами серебра на течение раневого процесса, а также его гепато-и нефротоксические эффекты.Материалы и методы. Для исследования были использованы образцы перевязочного средства на основе высокомолекулярных полимеров, которые представляли собой высокопористую губку из хитозан-коллагенового полиэлектролитного комплекса с включением коллоидных (ультрадисперсных) частиц металлического (нульвалентного) серебра. Эксперимент был выполнен на белых крысах-самцах породы «Вистар». Оценка результатов проводилась на основании планиметрического, бактериологического, гистологического, биохимического и гематологического методов. Результаты обработаны статистически.Результаты. Было выявлено, что к 15 суткам площадь ран уменьшилась на 96% от исходного размера, максимальная скорость заживления отмечалась на отрезке 1-5 сутки лечения, при гематологическом и биохимическом исследовании крови значимых изменений выявлено не было. При гистологическом исследовании микропрепаратов ран было отмечено, что к 15 суткам имел место разгар пролиферативной фазы воспаления, данные также подтверждены на основании морфометрического исследования и гидроксипролинового теста.Заключение. Таким образом, применение хитозан-коллагенового комплекса с наночастицами серебра положительно влияло на течение раневого процесса путем сокращения микробной обсемененности раны, стимулирования регенерации тканей, при этом не оказывая пагубного воздействия на печень и почки.Ключевые слова: лечение ран, хитозан-коллагеновый комплекс, наночастицы серебра, коллоидное серебро, раневое покрытие, раневой процесс. Бежин Александр

show abstract

unclassified

Влияние Хитозан-Коллагенового Комплекса С Наночастицами Серебра На Течение Раневого Процесса В Эксперименте

Бежин¹,

Липатов²,

Фрончек³

et al. 2019

Курск. науч.-практ. вестн. «Человек и его здоровье»

View full text Add to dashboard Cite

show abstract

Innovative biologically active wound healing and hemostatic agents based on chitosan and collagen: development stages, medical and technical characteristics

Фрончек¹,

Grigoryan

Блатун

2020

Rany i ranevye infekcii

View full text Add to dashboard Cite

Preservation of a consistently high number of domestic, industrial or military injuries, trophic ulcers, pressure sores, chronic wound defects in the morbidity structure makes the problem of effective and physiological healing of wounds extremely urgent. One of the most promising directions for solving this problem is the creation of wound healing and hemostatic agents of high efficiency (in comparison with traditional dressings from cellulose and non-woven textile materials) based on biologically active high-molecular natural and synthetic polymers with the immobilization of drugs with antibacterial, analgesic and necrolytic effects.Object: development of innovative biologically active wound healing and hemostatic agents based on chitosan and collagen.Materials and methods. As materials for determining medical and technical characteristics, new wound products were investigated: - means “Chitocol” performed by “Chitocol-S”, “Chitocol-CS”, “Chitocol-DA”, “Chitocol-CDA”, “Chitocol-SC”, “Chitocol-SCD”, sterile, “Chitocol-CSC”, “Chitocol-CSCD” with immobilized substances of colloidal silver, dioxidine and chymotrypsin; - means “Chitocol-Hemo” performed “Chitocol-Hemo C”, “Chitocol-Hemo CS”, “Chitocol-Hemo”, “Chitocol-Hemo S” with the immobilized substance colloidal silver. A preliminary preclinical evaluation of the effectiveness of the wound healing and antimicrobial effects of the prototype “Chitocol-CDA”, “Chitocol-CS” and “Chitocol-CSC” (porous plates 2×2×0.4 cm in size) in vivo was performed on white male Wistar rats weighing 180–200 g. The healing efficiency was evaluated on the basis of planimetric, hematological, histological research methods.Results. Quantitative indicators of medical and technical characteristics of the “Chitocol” and “Chitocol-Hemo” for use in the development of regulatory, technological and operational documentation on medical devices. An experimental study of wound dressings “Chitocol-CS”, “Chitocol-CDA” and “Chitocol-CSC” showed that their use leads to a reduction in the area of wounds by more than 90 % by 15 days of treatment, the maximum healing speed when applying the first two samples accounted for the first phase of the wound process (1–5 days), and in the group with purulent-necrotic process on 10–15 days of treatment. In the treatment of clean and infected wounds with the “Chitocol-CS” and “Chitocol-CDA” samples, complete healing and completion of the epithelization process began by 15 days, and in the group using “Chitocol-CSC” the proliferative phase of inflammation was in full swing.Conclusion. Quantitative indicators of medical and technical characteristics of innovative biologically active wound healing and hemostatic agents based on a (bio) polyelectrolyte complex of chitosan and collagen or chitosan with immobilized antimicrobial (dioxidine, colloidal silver), analgesic (anilokain) and proteolytic (chymotrypsin) positive results, as well preliminary preclinical study of wound healing agents with dioxidine and anilokain (“Chitocol-CDA”), colloidal silver (“Chitocol-CS”), colloidal silver and chymotrypsin (“Chitocol-CSC”) provide the basis for continuing work on toxicological, acceptance technical preclinical and clinical trials with the aim of experimental confirmation of the expected effectiveness and safety of the developed products.

show abstract

Morphological evaluation of the effectiveness of the use of chitosan-collagen complex with silver nano-particles and chymotrypsin in the purulent-necrotic process in soft tissues

Бежин

Липатов

Блатун

et al. 2020

Rany i ranevye infekcii

View full text Add to dashboard Cite

One of the urgent problems of modern surgery continues to be the increasing number of patients with purulent-inflammatory and purulentnecrotic processes of soft tissues – about 60.0 % of patients in the structure of specialized hospitals of a surgical profile. This is due to the adaptation of microorganisms – pathogens of wound infection to existing means for treating wounds. In addition, the number of patients with diabetes mellitus increases annually, and as a result, the number of people suffering from diabetic foot syndrome. Thus, the relevance of developing new modern tools for the local treatment of purulent-necrotic processes of the skin and soft tissues is not reduced.Materials and methods. The material for the study was a highly porous biodegradable sponge (chitosan-collagen) in the ratio of chitosan: collagen 2: 1 with the inclusion of colloidal particles of metallic silver and chymotrypsin. The experiment was performed on 50 Wistar rats that simulated a purulent necrotic wound. During the treatment, the state of the wounds was visually evaluated, the morphological features of the course of the wound process were evaluated after animals were withdrawn from the experiment and the wound material was taken on days 1, 5, 10, 15 and 21, the sections were stained with hematoxylin-eosin and/or according to Van Gieson.Results. By visual assessment, by the 10th day it was noted that necrotic tissues underwent lysis, the wound defect was covered with a scab, swelling of the surrounding tissues was not noted, and on the 21st day of treatment the wound defect was covered with epithelium and had an area of about 3 mm2 with the initial - in 250 mm2. When describing micropreparations, it was noted that on the 10th day the exudation phase passed its peak and the activity of proliferation processes increased from the center of the wound to its periphery and from the granulation surface in depth. On the 15th day, the proliferative phase of inflammation was at its height, and on the 21st day, the proliferation phase was completed and the processes of remodeling of dense fibrous connective tissue began.Conclusion. Based on a visual assessment of the state of wounds and a morphological study, the effectiveness of a chitosan-collagen complex with silver nanoparticles and chymotrypsin was shown to be applied locally to a purulent necrotic wound in an experiment.

show abstract

In Vivo and in Vitro Studies of the Effect of Chitosan on the Bone Defect Repair Process

Cited by 4 publications

References 14 publications

Влияние Хитозан-Коллагенового Комплекса С Наночастицами Серебра На Течение Раневого Процесса В Эксперименте

Влияние Хитозан-Коллагенового Комплекса С Наночастицами Серебра На Течение Раневого Процесса В Эксперименте

Innovative biologically active wound healing and hemostatic agents based on chitosan and collagen: development stages, medical and technical characteristics

Morphological evaluation of the effectiveness of the use of chitosan-collagen complex with silver nano-particles and chymotrypsin in the purulent-necrotic process in soft tissues

Contact Info

Product

Resources

About