In vivo and in vitro insulin and fasting control of the transmembrane fatty acid transport proteins in Atlantic salmon (Salmo salar)

Abstract

(FABP3), and mitochondrial FA-CoA and -carnitine palmitoyl transferase carriers (CPT)1 and -2 in Atlantic salmon tissues and myocyte cell culture. Two weeks of fasting diminished FATP1, CD36, and LPL in adipose tissue, suggesting a reduction in FA uptake, while FABP3 increased in liver, probably enhancing the transport of FA to the mitochondria. Insulin injection decreased FATP1 and CD36 in white and red muscles, while both transporters were upregulated in the adipose tissue in agreement with the role of insulin-inhibiting muscle FA oxidation and stimulating adipose fat stores. Serum deprivation of 48 h in Atlantic salmon myotubes increased FATP1, FABP3, and CPT-2, while CPT-1 was diminished. In myotubes, insulin induced FATP1 expression but decreased CD36, FABP3, and LPL, suggesting that FATP1 could be more involved in the insulin-stimulated FA uptake. Insulin increased the FA uptake in myotubes mediated, at least in part, through the relocation of FATP1 protein to the plasma membrane. Overall, Atlantic salmon FA transporters are regulated by fasting and insulin on in vivo and in vitro models.

Effects of insulin treatment on the response to oleate and octanoate of food intake and fatty acid–sensing systems in rainbow trout

“…In liver, insulin treatment resulted in decreased concentration of FA, triglyceride, glucose, and glycogen, and increased mRNA abundance of FAT/CD36, FAS, PPARa, and SREBP1c. These changes are in agreement with those previously observed in vivo in the same [27][28][29]33,45] and other fish species [20,46] after insulin treatment. Moreover, other variables that had not been assessed in comparable studies in fish liver before, such as decreased mRNA abundance of UCP2a or SUR-like are indicative of decreased mitochondrial activity elicited by insulin treatment.…”

Comparison of Glucose and Lipid Metabolic Gene Expressions between Fat and Lean Lines of Rainbow Trout after a Glucose Load

“…Even though we cannot eliminate a putative difference linked to the generation of selection (5th vs. 3rd and 4th), these discrepancies could be due to the nutritional status of the fish, because this is the first time that we analyzed fasted fish (48 h prior to the peritoneal injection). Indeed, unfed fish use fatty acids as the preferential energy source [60] in contrast to fed fish; we can hypothesize that fatty acid potential could be strongly changed in this context in the fish lines.…”

Cloning and characterization of fatty acid transport proteins in Japanese seabassLateolabrax japonicus, and their gene expressions in response to dietary arachidonic acid

“…The regulation of fish FATPs also remains greatly unexplored. Only recently, few studies have been reported on the regulation of FATPs expression in Atlantic salmon and rainbow trout (S anchez-Gurmaches, Cruz-Garcia, Guti errez & Navarro, 2012;S anchez-Gurmaches et al, 2011;Todor cevi c et al, 2008;Torstensen, Nanton, Olsvik, Sundvold & Stubhaug, 2009). It has been suggested that insulin, fasting, and inflammation and infection mediators could regulate the gene expression of fish FATP1.…”