In Vivo and In Vitro Effects of Tea Extracts on Enterotoxigenic <i>Escherichia coli</i>‐induced Intestinal Fluid Loss in Animal Models

Bruins, Maaike J.; Cermak, Rainer; Kiers, J.L.; Meulen, Jan van der; Amelsvoort, J.M.M. van; Klinken, B. J.-W. Van

doi:10.1097/01.mpg.0000239992.12646.df

Cited by 45 publications

(35 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, and Cland increased net secretion of K ? compared with controls [5]. In the present report by Lodemann et al, the responses of I sc after stimulation with the secretagogue PGE 2 or glucose were reduced in ETEC-treated jejunum, reinforcing the proposition that transport functions were impaired.…”

supporting

confidence: 80%

Early Events of Enterotoxigenic Escherichia coli Colonization on Gut Barrier Function: No Longer UndETECted?

Carrasco

Fernández‐Miyakawa

2017

Dig Dis Sci

View full text Add to dashboard Cite

supporting

confidence: 80%

Early Events of Enterotoxigenic Escherichia coli Colonization on Gut Barrier Function: No Longer UndETECted?

Carrasco

Fernández‐Miyakawa

2017

Dig Dis Sci

View full text Add to dashboard Cite

“…Most studies employing SISP, however, focused on analysis of physiological and immune responses of the host, including fluid loss, electrolyte secretion, and the expression of cytokines (23,26,27,30). Very few studies provided a detailed bacteriological analysis of the model; previous studies using the SISP model reported only cell counts of ETEC in the lumen (27,31,32). To determine whether glycans prevent pathogen adhesion, we focused on the quantification of mucosa-adherent ETEC.…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharides Synthesized by Lactobacillus reuteri Protect against Enterotoxigenic Escherichia coli in Piglets

Chen

Woodward

Zijlstra

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bEnterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in piglets; ETEC cells colonize the intestinal mucosa with adhesins and deliver toxins that cause fluid loss. This study determined the antiadhesive properties of bacterial exopolysaccharides (reuteran and levan) and related glycans (dextran and inulin) in a small intestinal segment perfusion (SISP) model. The SISP model used 10 jejunal segments from 5-week-old piglets. Five segments were infected with ETEC expressing K88 fimbriae (ETEC K88), while five segments were treated with saline. Every two segments (ETEC and non-ETEC infected) were infused with 65 ml of 10 g liter ؊1 of glycans or saline (control) for 8 h. High-resolution melting-curve (HRM) quantitative PCR (qPCR) indicated that E. coli is the dominant bacterium in infected segments, while other bacteria were predominant in noninfected segments. Infection by ETEC K88 was also verified by qPCR; gene copy numbers of K88 fimbriae and the heat-labile toxin (LT) in mucosal scrapings and outflow fluid of infected segments were significantly higher than those in noninfected segments. Genes coding for K88 fimbriae and LT were also detected in noninfected segments. LT amplicons from infected and noninfected segments were 99% identical over 481 bp, demonstrating the presence of autochthonous ETEC K88. All glycans reduced fluid loss caused by ETEC K88 infection. Reuteran tended (P ‫؍‬ 0.06) to decrease ETEC K88 levels in mucosal scraping sample, as judged by qPCR. Fluorescent in situ hybridization analysis demonstrated that reuteran significantly (P ‫؍‬ 0.012) decreased levels of adherent ETEC K88. Overall, reuteran may prevent piglet diarrhea by reducing adhesion of ETEC K88.

show abstract

“…Different researches have been reported that camellia sinensis has antimicrobial activities against various pathogenic bacteria [1][2][3][4][5][6][7][8][9]. Tea could be categorized into three main types, depending on the level of oxidation, as green tea, oolong tea and black tea [10].…”

Section: Introductionmentioning

confidence: 99%

New wound dressing polymeric nanofiber containing green tea extract prepared by electrospinning method

et al. 2015

View full text Add to dashboard Cite

The effect of green tea extract as a natural and ecofriendly antibacterial additive on the healing effect of chitosan/ polyethylene oxide wound dressing nanofiber was investigated. Three different polymeric nanofiber composite including chitosan, chitosan/polyethylene oxide and chitosan/polyethylene oxide/green tea extract, was produced by electrospinning method and was used as wound dressing. Affecting parameters on electrospinning method such as voltage, feed rate, nozzlecollector distance and content of green tea in nanofiber structure were studied and optimized. The morphology of electrospun nanofibers was studied with use of scanning electron microscope. The average diameters of prepared nanofibers was determined by Image J software. The optimum conditions for preparation of electrospun nanofibers were: Voltage; 20 kV, feed rate; 0.5 ml/h, nozzle-collector distance; 10 cm, and chitosan/polyethylene oxide weight ratio; 0.9. Stability, water absorption and degradation of prepared nanofibers were investigated by placing the prepared nanofibers for 24 hours in a solution of acetate buffer with a pH of 5.5 and a temperature of 37 o C. Moreover, the antibacterial activity of composite scaffolds were tested by Agar plate method by two type bacteria including Escherichia coli and Staphylococcus aureus. Furthermore, the healing ability of the prepared nanofibers was studied on the rat's wound. Chitosan/polyethylene oxide/ green tea showed the best healing effects in comparison with the other prepared wound dressings. These results confirmed that green tea extract helps to keep wound surface moist, reduces inflammation and increases the speed of recovery and healing.

show abstract

In Vivo and In Vitro Effects of Tea Extracts on Enterotoxigenic Escherichia coli‐induced Intestinal Fluid Loss in Animal Models

Abstract: Tea may inhibit net fluid and electrolyte losses involved in secretory diarrhoea from ETEC.

Cited by 45 publications

References 31 publications

Early Events of Enterotoxigenic Escherichia coli Colonization on Gut Barrier Function: No Longer UndETECted?

Early Events of Enterotoxigenic Escherichia coli Colonization on Gut Barrier Function: No Longer UndETECted?

Exopolysaccharides Synthesized by Lactobacillus reuteri Protect against Enterotoxigenic Escherichia coli in Piglets

New wound dressing polymeric nanofiber containing green tea extract prepared by electrospinning method

Contact Info

Product

Resources

About